黄芪皂甙对人系膜细胞基质分泌和β1整合素表达的影响
作者：倪兆慧 张庆怡 钱家麒 王利民 王健华
单位：200001 上海第二医科大学仁济医院肾脏科 分子生物实验室
关键词：黄芪；β1整合素；胶原Ⅳ型；系膜细胞
中华肾脏病杂志000507摘 要：目的了解黄芪皂甙对人肾小球系膜细胞(HMC)的影响。方法应用不同浓度的黄芪皂甙或黄芪皂甙血清处理经人重组血小板衍化生长因子(PDGF-BB)刺激的培养的人系膜细胞，分别采用四甲基偶氮唑蓝(MTT)法和酶联免疫吸附(ELISA)方法检测系膜细胞的增殖和Ⅳ型胶原分泌情况，用逆转录-聚合酶链反应(RT-PCR)法检测β1整合素基因的表达。结果经黄芪皂甙或黄芪血清处理72h后，由PDGF-BB诱导的系膜细胞的增殖与未经处理的对照组相比呈显著抑制，并呈剂量依赖效应(P<0.05)；黄芪皂甙或黄芪血清作用24h后，经PDGF-BB诱导的系膜细胞分泌的Ⅳ型胶原蛋白浓度和β1整合素mRNA水平均较未经用药的对照组有显著下降，并与剂量呈一定相关(P<0.05和P<0.01)；β1整合素mRNA表达下调与人肾小球系膜细胞增殖抑制和Ⅳ型胶原蛋白浓度分泌下降之间存在显著性相关(P<0.05和P<0.01)。结论黄芪皂甙可抑制人肾小球系膜细胞增殖，抑制基质的过度生成和下调细胞表面β1整合素mRNA的表达。
, 百拇医药
Effect of Astragalin on matrix secretion and β1 Integrin mRNA expression in human mesangial cells
NI Zhaohui ,ZHANG Qingyi ,QIAN Jiaqi,et al.
(Renal Division,Department of Molecular Biology,Renji Hospital,Shanghai Second Medical University,Shanghai 200001,China)
Abstract：Objective To investigate the effect of Astragalin on human renal mesangial cells.Methods Cultured human mesangial cells were treated with Astragalin and Astragalin serum in different concenrations in the presence of PDGF-BB,the proliferation and type Ⅳ collagen secretion of mesangial cells were measured by MTF assay and ELISA,and expression of β1 integrin gene was estimated by RT-PCR method,respectively.Results After 72 hours of Astragalin or Astragalin serum treatment,the proliferation of mesangial cells induced by PDGF-BB was inhibited significantly in a dose-dependent manner compared with untreated control(P<0.05).After 24 hours of Astragalin or Astragalin serum treatment,the secretion of type Ⅳ collagen protein in the presence of PDGF-BB was significantly decreased and β1 integfin mRNA level decreased significantly compared with untreated control(P<0.05 and P<0.01).Down-regulation of β1 integrin mRNA expression was significantly correlated with inhibition of mesangial cell proliferation and decrease of the type Ⅳ collagen protein secretion(P<0.05 and P<0.01).Conclusion Astragalin can inhibit proliferation and matrix over-synthesis of HMC,and down-regulate expression of β1 integrin on surface of HMC.
, 百拇医药
Keywords：Astragalius; β1 integrin;Collagen type Ⅳ;Mesangial cell 基金项目：上海市卫生局基金资助项目(98A302)
参考文献：
[1]Rupprecht HD,Schocklmann HO,Sterzel RB.Cell-matrix interaction in the glomerular mesangium.Kidney Int,1996,49:1575-1582.
[2]Gauer S,Yao J,Schocklmann HO,et al.Adhesion molecules in the glomerular mesangium.Kidney Int,1997.51:1447-1453.
[3]徐郁杰，张庆怡，吴青伟.黄芪对糖尿病大鼠早期肾肥大和蛋白尿的影响.上海第二医科大学学报，1997，17：357-359.
, 百拇医药
[4]Gqmez GC,Gonzalez C,Egido J.Evidence for a specific IgA receptor in rat and human mesangial cells.J Immunol,1993,151:7172-7279.
[5]王宁生，雷燕，刘平，等.关于血清药理学的若干问题.中国中西医结合杂志，1999，19:263-266.
[6]Hansen MB,Nielsen SE,Berg K.Re-examination and further development of a precise and rapid dye method for measuring cell growth/cell kill.J Immunol Methods,1989,119:203-211.
[7]Kasho M,Sakai M,Sasahara T,et al.Serotonin enhances the production of type Ⅳ collagen by human mesangial cells.Kidney Int,1998,54:1083-1092.
, 百拇医药
[8]Rozzo C,Chiesa V,Caridi G,et al.Induction of apoptosis in human neuroblastoma cells by abrogation of integrin-mediated cell adhesion.Int J Cancer,1997,70: 688-698.
[9]徐郁杰，张庆怡，陆敏，等.黄芪对糖尿病大鼠肾皮质TGF-β表达的影响.中华内分泌代谢杂志，1998，14：312-314.
[10]Marx M,Daniel TO,Kashgarian M,et al.Spatial organization of the extracellular matrix modulates the exoerssion of PDGF subunits in mesangial cells.Kidney Int,1993,43:1027-1041.
收稿日期：1999-11-20, http://www.100md.com
单位：200001 上海第二医科大学仁济医院肾脏科 分子生物实验室
关键词：黄芪；β1整合素；胶原Ⅳ型；系膜细胞
中华肾脏病杂志000507摘 要：目的了解黄芪皂甙对人肾小球系膜细胞(HMC)的影响。方法应用不同浓度的黄芪皂甙或黄芪皂甙血清处理经人重组血小板衍化生长因子(PDGF-BB)刺激的培养的人系膜细胞，分别采用四甲基偶氮唑蓝(MTT)法和酶联免疫吸附(ELISA)方法检测系膜细胞的增殖和Ⅳ型胶原分泌情况，用逆转录-聚合酶链反应(RT-PCR)法检测β1整合素基因的表达。结果经黄芪皂甙或黄芪血清处理72h后，由PDGF-BB诱导的系膜细胞的增殖与未经处理的对照组相比呈显著抑制，并呈剂量依赖效应(P<0.05)；黄芪皂甙或黄芪血清作用24h后，经PDGF-BB诱导的系膜细胞分泌的Ⅳ型胶原蛋白浓度和β1整合素mRNA水平均较未经用药的对照组有显著下降，并与剂量呈一定相关(P<0.05和P<0.01)；β1整合素mRNA表达下调与人肾小球系膜细胞增殖抑制和Ⅳ型胶原蛋白浓度分泌下降之间存在显著性相关(P<0.05和P<0.01)。结论黄芪皂甙可抑制人肾小球系膜细胞增殖，抑制基质的过度生成和下调细胞表面β1整合素mRNA的表达。
, 百拇医药
Effect of Astragalin on matrix secretion and β1 Integrin mRNA expression in human mesangial cells
NI Zhaohui ,ZHANG Qingyi ,QIAN Jiaqi,et al.
(Renal Division,Department of Molecular Biology,Renji Hospital,Shanghai Second Medical University,Shanghai 200001,China)
Abstract：Objective To investigate the effect of Astragalin on human renal mesangial cells.Methods Cultured human mesangial cells were treated with Astragalin and Astragalin serum in different concenrations in the presence of PDGF-BB,the proliferation and type Ⅳ collagen secretion of mesangial cells were measured by MTF assay and ELISA,and expression of β1 integrin gene was estimated by RT-PCR method,respectively.Results After 72 hours of Astragalin or Astragalin serum treatment,the proliferation of mesangial cells induced by PDGF-BB was inhibited significantly in a dose-dependent manner compared with untreated control(P<0.05).After 24 hours of Astragalin or Astragalin serum treatment,the secretion of type Ⅳ collagen protein in the presence of PDGF-BB was significantly decreased and β1 integfin mRNA level decreased significantly compared with untreated control(P<0.05 and P<0.01).Down-regulation of β1 integrin mRNA expression was significantly correlated with inhibition of mesangial cell proliferation and decrease of the type Ⅳ collagen protein secretion(P<0.05 and P<0.01).Conclusion Astragalin can inhibit proliferation and matrix over-synthesis of HMC,and down-regulate expression of β1 integrin on surface of HMC.
, 百拇医药
Keywords：Astragalius; β1 integrin;Collagen type Ⅳ;Mesangial cell 基金项目：上海市卫生局基金资助项目(98A302)
参考文献：
[1]Rupprecht HD,Schocklmann HO,Sterzel RB.Cell-matrix interaction in the glomerular mesangium.Kidney Int,1996,49:1575-1582.
[2]Gauer S,Yao J,Schocklmann HO,et al.Adhesion molecules in the glomerular mesangium.Kidney Int,1997.51:1447-1453.
[3]徐郁杰，张庆怡，吴青伟.黄芪对糖尿病大鼠早期肾肥大和蛋白尿的影响.上海第二医科大学学报，1997，17：357-359.
, 百拇医药
[4]Gqmez GC,Gonzalez C,Egido J.Evidence for a specific IgA receptor in rat and human mesangial cells.J Immunol,1993,151:7172-7279.
[5]王宁生，雷燕，刘平，等.关于血清药理学的若干问题.中国中西医结合杂志，1999，19:263-266.
[6]Hansen MB,Nielsen SE,Berg K.Re-examination and further development of a precise and rapid dye method for measuring cell growth/cell kill.J Immunol Methods,1989,119:203-211.
[7]Kasho M,Sakai M,Sasahara T,et al.Serotonin enhances the production of type Ⅳ collagen by human mesangial cells.Kidney Int,1998,54:1083-1092.
, 百拇医药
[8]Rozzo C,Chiesa V,Caridi G,et al.Induction of apoptosis in human neuroblastoma cells by abrogation of integrin-mediated cell adhesion.Int J Cancer,1997,70: 688-698.
[9]徐郁杰，张庆怡，陆敏，等.黄芪对糖尿病大鼠肾皮质TGF-β表达的影响.中华内分泌代谢杂志，1998，14：312-314.
[10]Marx M,Daniel TO,Kashgarian M,et al.Spatial organization of the extracellular matrix modulates the exoerssion of PDGF subunits in mesangial cells.Kidney Int,1993,43:1027-1041.
收稿日期：1999-11-20, http://www.100md.com