IL-1βmRNA在胎儿脑及U251细胞中的表达
作者:孙英 刘素清 卢志远 李凤山 鲍永利
单位:孙英 刘素清 卢志远 李凤山(110042 辽宁省沈阳市,中国人民解放军第四六三医院妇产科);鲍永利(白求恩医科大学免疫教研室)
关键词:IL-1βmRNA;斑点杂交法;Nonhern;blot;胎儿脑
中国煤炭工业医学杂志000964摘 要:目的探讨IL-1βmRNA在胎儿脑中的表达与某些疾病的关系。方法采用杂交法检测胎儿脑中IL-1βmRNA的水平,利用Nonhem杂交法检测人脑胶质瘤传代细胞U251中IL-1β的表达情况。结果显示了TPA可明显刺激U251中IL-1β的表达。结论孕2个月胎儿脑在未加任何刺激的自然情况下即有中IL-1β的表达。
EXPRESSION OF IL-1βMRNA IN FETAL BRAIN AND ASTROCYTOMA U251
Sun Ying ,Liu Suqing , Lu Zhiyuan
(The 463rd Hospital of PLA, Shen Yang 110042)
Abstract:Abstract Objective To study the relationship between the level of IL-1βmRNA in fetal brain and certain diseases. Methods The level of IL-1βmRNA in fetal brain was determined with dot blot and IL-1βexpression in cell U251 was determined by Northern blot. Results IL-1βmRNA from U251 cell stimulated by TPA showed a significant expression. Conclusion The IL-1βmRNA in fetal brain within 2 months of conception will show expressibn without any stimulation.
Keywords:IL-1βmRNA Dot blot Northern blot Fetal brain
参考文献:
[1]锡村明生.神经系のサィトカィン ネツトフ一ク.实验医学,1993,11(10):133-139.
[2]今西二郎.抗肿瘤性サィトカィン.日本临床,1992,50(8):45-49.
[3]枥泽史郎.IL-1の抗肿瘤作用,临床免疫,1989,21(4):479-485.
收稿日期:1999-11-08
修稿日期:2000-03-28, 百拇医药
单位:孙英 刘素清 卢志远 李凤山(110042 辽宁省沈阳市,中国人民解放军第四六三医院妇产科);鲍永利(白求恩医科大学免疫教研室)
关键词:IL-1βmRNA;斑点杂交法;Nonhern;blot;胎儿脑
中国煤炭工业医学杂志000964摘 要:目的探讨IL-1βmRNA在胎儿脑中的表达与某些疾病的关系。方法采用杂交法检测胎儿脑中IL-1βmRNA的水平,利用Nonhem杂交法检测人脑胶质瘤传代细胞U251中IL-1β的表达情况。结果显示了TPA可明显刺激U251中IL-1β的表达。结论孕2个月胎儿脑在未加任何刺激的自然情况下即有中IL-1β的表达。
EXPRESSION OF IL-1βMRNA IN FETAL BRAIN AND ASTROCYTOMA U251
Sun Ying ,Liu Suqing , Lu Zhiyuan
(The 463rd Hospital of PLA, Shen Yang 110042)
Abstract:Abstract Objective To study the relationship between the level of IL-1βmRNA in fetal brain and certain diseases. Methods The level of IL-1βmRNA in fetal brain was determined with dot blot and IL-1βexpression in cell U251 was determined by Northern blot. Results IL-1βmRNA from U251 cell stimulated by TPA showed a significant expression. Conclusion The IL-1βmRNA in fetal brain within 2 months of conception will show expressibn without any stimulation.
Keywords:IL-1βmRNA Dot blot Northern blot Fetal brain
参考文献:
[1]锡村明生.神经系のサィトカィン ネツトフ一ク.实验医学,1993,11(10):133-139.
[2]今西二郎.抗肿瘤性サィトカィン.日本临床,1992,50(8):45-49.
[3]枥泽史郎.IL-1の抗肿瘤作用,临床免疫,1989,21(4):479-485.
收稿日期:1999-11-08
修稿日期:2000-03-28, 百拇医药