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中国现代医学杂志001001Obstructed Bile Duct as a Trigger
for Microbe's Translocation?
Hu Jixiong
(Department. Of General Surgery, the Second Affiliated Hospital,Hunan Medical University, Changsha, P.R.China)
Zhang Yangde
(National hepatobiliary & Enteric Surgery Research Center,Ministry of Health P.R.China)
Objective: To assess the potential mechanisms of bacterial translocation in a murine model of obstructive jaundice. Methods: Adult rats were randomized to be operated on for ligation or sham-ligation of the common bile duct. Bacterial translocation to the mesenteric lymph nodes (MLNs), liver, spleen, portal blood and systemic circulation and bacterial population levels in the ceca were quantitated after 7 and 14 days. The terminal ilea were histologically examined by light and transmission electron microscopy. Results: Bacterial translocation to the MNLs was seen in both 7 (10/17) and 14 (11/18) day ligated animals, but not found in their corresponding controls (both 0/8). No significant difference in the cecal bacterial population levels was found between the ligated groups and their corresponding control groups, also between the two subgroups that were set up within each ligated group according to the presence or absence of bacteria in the MLNs. In the ligated rats, light microscopy demonstrated subepithelial edema in association with infiltration of flammatory cells and, transmission electron microscopy showed that the enterocytes were injured with abnormal microvilli, swollen mitochondria, unclear endoplasmic reticulum and cytoplasm with bubble degeneration. However, the ilea from the controls appeared normal. Conclusions: Obstructive jaundice promotes bacterial translocation in rats. The gut mucosal damage rather than the intestinal bacterial overgrowth may play a crucial role in bacterial translocation. Under normal conditions, the host has several different defense mechanisms to pr event bacteria colonizing the gut from directly adhearing to the intestinal muco sa or translocating through the mucosa to the extraintestinal organs and tissues . These mechanisms include mechanical defenses, immunologic defenses, and the ab ility of the indigenous intestinal microflora to prevent colonization by exogeno us pathogens or overgrowth by certain indigenous pathogens (termed conolization resistance)[1]. When these host-defense mechanisms are sufficiently pert urbed, m icroorganisms normally confined within the gut lumen have the capability of migr ating to the mesenteric lymph nodes and other tissues, a process termed bacteria l translocation[2,3]. This phenomenon has been presumed to be a potentia l m echanism of sepsis and multiple organ failure in critically ill patients[4 ～6]. ......
您现在查看是摘要页，全文长 29085 字符。
单位：
关键词：
中国现代医学杂志001001Obstructed Bile Duct as a Trigger
for Microbe's Translocation?
Hu Jixiong
(Department. Of General Surgery, the Second Affiliated Hospital,Hunan Medical University, Changsha, P.R.China)
Zhang Yangde
(National hepatobiliary & Enteric Surgery Research Center,Ministry of Health P.R.China)
Objective: To assess the potential mechanisms of bacterial translocation in a murine model of obstructive jaundice. Methods: Adult rats were randomized to be operated on for ligation or sham-ligation of the common bile duct. Bacterial translocation to the mesenteric lymph nodes (MLNs), liver, spleen, portal blood and systemic circulation and bacterial population levels in the ceca were quantitated after 7 and 14 days. The terminal ilea were histologically examined by light and transmission electron microscopy. Results: Bacterial translocation to the MNLs was seen in both 7 (10/17) and 14 (11/18) day ligated animals, but not found in their corresponding controls (both 0/8). No significant difference in the cecal bacterial population levels was found between the ligated groups and their corresponding control groups, also between the two subgroups that were set up within each ligated group according to the presence or absence of bacteria in the MLNs. In the ligated rats, light microscopy demonstrated subepithelial edema in association with infiltration of flammatory cells and, transmission electron microscopy showed that the enterocytes were injured with abnormal microvilli, swollen mitochondria, unclear endoplasmic reticulum and cytoplasm with bubble degeneration. However, the ilea from the controls appeared normal. Conclusions: Obstructive jaundice promotes bacterial translocation in rats. The gut mucosal damage rather than the intestinal bacterial overgrowth may play a crucial role in bacterial translocation. Under normal conditions, the host has several different defense mechanisms to pr event bacteria colonizing the gut from directly adhearing to the intestinal muco sa or translocating through the mucosa to the extraintestinal organs and tissues . These mechanisms include mechanical defenses, immunologic defenses, and the ab ility of the indigenous intestinal microflora to prevent colonization by exogeno us pathogens or overgrowth by certain indigenous pathogens (termed conolization resistance)[1]. When these host-defense mechanisms are sufficiently pert urbed, m icroorganisms normally confined within the gut lumen have the capability of migr ating to the mesenteric lymph nodes and other tissues, a process termed bacteria l translocation[2,3]. This phenomenon has been presumed to be a potentia l m echanism of sepsis and multiple organ failure in critically ill patients[4 ～6]. ......
您现在查看是摘要页，全文长 29085 字符。