IFNγ和IL—6对人肝癌细胞系C1抑制物合成的调控
作者：陈晓韧 谢佩蓉 郑 萍9xm, http://www.100md.com
单位：第三军医大学免疫学教研室，重庆 4000389xm, http://www.100md.com
关键词：C1抑制物；肝癌细胞系；IFNγ；IL—69xm, http://www.100md.com
摘 要摘 要：目的为研究IFNY或IL—6对肝癌细胞系C1抑制物(C1INH)合成的调控作用。方法 用ELISA双夹心法检测了经其刺激后HepG2和SMMC7712细胞培养上清中C1lNH的含量。结果两细胞系自身能合成少量C1INH。IFNγ和IL-6可通过不同的途径上调两种细胞C1INH的合成，且IFNγ的作用强于IL—6。结论此结果提示有可能用IFNγ和IL—6治疗C1LNB缺乏病。9xm, http://www.100md.com
分类号：R392.11 文献标识码：A9xm, http://www.100md.com
文章编号：1000—8861(2000)02—0084—049xm, http://www.100md.com
作者简介：陈晓韧(1970-)，女，四川乐山市人，主治医师，硕士，主要从事分子免疫学研究，现在空军总医院检验科工作。9xm, http://www.100md.com
参考文献：9xm, http://www.100md.com
[1]JOHN E VOLANAKIS. Tkanscriptional regulation of complement genes [J] . Annu Rev Immunol, 1995,13:277～305.9xm, http://www.100md.com
[2]谢佩蓉，郑萍，姜曼，等，抗人C1-INH单克隆抗体的制备及其在分离人血清中C1-INH的作用[J].免疫学杂志,1991,7(1)22～25.9xm, http://www.100md.com
[3]陈晓韧,调佩蓉,郑萍,等.IFNγ对U937细胞C1抑制物合成调控作用的研究[J]. 免疫学杂志，1998，(4):250～252.9xm, http://www.100md.com
[4]PETER CH,JOSE VC,TILO A, Interleukin 6 and the acute phase response [J]. Biochem [J], 1990,265: 621～636.9xm, http://www.100md.com
[5]BAUER J,BAUER TM,KALB T,et al, Regulation of interleukin 6 receptor expression in human monocytes and monocyte-derived macrophages [J]. J Exp Med,1989,170:1537～1549.9xm, http://www.100md.com
[6]BRUCE LZ,MARTIN L. Regulation of the hepatic synthesis of C1 inhibitor by the hepatocyte stimula ting factors interleukin 6 and interferon γ[J]. J Bio Chem, 1990,265:12664～12670.9xm, http://www.100md.com
[7]ZOLTAN .NO,EDWARD CCW,JOHN PA,et al. Biology of disease,C1 inhibitor deficiency: molecular and immunologic basis of hereditary and acquired an-gioedema[J]. Lab Invest, 1991,65: 383～388.9xm, http://www.100md.com
[8]VIRGINIA HD,JOHN .JB, THOMAS RW,et al. An tibody to C1-inhibitor in a patient receiving C1-in-bibitor infusions for treatment of hereditary angioneu-rotic edema with systemic lupus erythomatosus reacts with a normal allotype of residue 458 of C1-inhibitor [J]. Lab Clin Med.1996,128:438～443.9xm, http://www.100md.com
[9]HEDA GD,MARDENTE S,WEINER L,et al, Inter feron-gamma increases in vitro and in vivo expression of C1 iahibitor[J]. Blood ,1990,75:2401～2407.9xm, http://www.100md.com
[10]JUDIT K,YITZHAK K,FRED SR,et al. Synthesis of C1 inhibitor in fibroblasts from patients with type Ⅰand type Ⅱ hereditary angioneurotic edema[J]. J Clin Invest. 1991.87:1614～1620.9xm, http://www.100md.com
收稿日期：1999-05-10(陈晓韧 谢佩蓉 郑 萍)
单位：第三军医大学免疫学教研室，重庆 4000389xm, http://www.100md.com
关键词：C1抑制物；肝癌细胞系；IFNγ；IL—69xm, http://www.100md.com
摘 要摘 要：目的为研究IFNY或IL—6对肝癌细胞系C1抑制物(C1INH)合成的调控作用。方法 用ELISA双夹心法检测了经其刺激后HepG2和SMMC7712细胞培养上清中C1lNH的含量。结果两细胞系自身能合成少量C1INH。IFNγ和IL-6可通过不同的途径上调两种细胞C1INH的合成，且IFNγ的作用强于IL—6。结论此结果提示有可能用IFNγ和IL—6治疗C1LNB缺乏病。9xm, http://www.100md.com
分类号：R392.11 文献标识码：A9xm, http://www.100md.com
文章编号：1000—8861(2000)02—0084—049xm, http://www.100md.com
作者简介：陈晓韧(1970-)，女，四川乐山市人，主治医师，硕士，主要从事分子免疫学研究，现在空军总医院检验科工作。9xm, http://www.100md.com
参考文献：9xm, http://www.100md.com
[1]JOHN E VOLANAKIS. Tkanscriptional regulation of complement genes [J] . Annu Rev Immunol, 1995,13:277～305.9xm, http://www.100md.com
[2]谢佩蓉，郑萍，姜曼，等，抗人C1-INH单克隆抗体的制备及其在分离人血清中C1-INH的作用[J].免疫学杂志,1991,7(1)22～25.9xm, http://www.100md.com
[3]陈晓韧,调佩蓉,郑萍,等.IFNγ对U937细胞C1抑制物合成调控作用的研究[J]. 免疫学杂志，1998，(4):250～252.9xm, http://www.100md.com
[4]PETER CH,JOSE VC,TILO A, Interleukin 6 and the acute phase response [J]. Biochem [J], 1990,265: 621～636.9xm, http://www.100md.com
[5]BAUER J,BAUER TM,KALB T,et al, Regulation of interleukin 6 receptor expression in human monocytes and monocyte-derived macrophages [J]. J Exp Med,1989,170:1537～1549.9xm, http://www.100md.com
[6]BRUCE LZ,MARTIN L. Regulation of the hepatic synthesis of C1 inhibitor by the hepatocyte stimula ting factors interleukin 6 and interferon γ[J]. J Bio Chem, 1990,265:12664～12670.9xm, http://www.100md.com
[7]ZOLTAN .NO,EDWARD CCW,JOHN PA,et al. Biology of disease,C1 inhibitor deficiency: molecular and immunologic basis of hereditary and acquired an-gioedema[J]. Lab Invest, 1991,65: 383～388.9xm, http://www.100md.com
[8]VIRGINIA HD,JOHN .JB, THOMAS RW,et al. An tibody to C1-inhibitor in a patient receiving C1-in-bibitor infusions for treatment of hereditary angioneu-rotic edema with systemic lupus erythomatosus reacts with a normal allotype of residue 458 of C1-inhibitor [J]. Lab Clin Med.1996,128:438～443.9xm, http://www.100md.com
[9]HEDA GD,MARDENTE S,WEINER L,et al, Inter feron-gamma increases in vitro and in vivo expression of C1 iahibitor[J]. Blood ,1990,75:2401～2407.9xm, http://www.100md.com
[10]JUDIT K,YITZHAK K,FRED SR,et al. Synthesis of C1 inhibitor in fibroblasts from patients with type Ⅰand type Ⅱ hereditary angioneurotic edema[J]. J Clin Invest. 1991.87:1614～1620.9xm, http://www.100md.com
收稿日期：1999-05-10(陈晓韧 谢佩蓉 郑 萍)