外科手术患者血中细菌DNA的检出率
作者：文利平 蒋朱明 唐伟松 梁存和 王树惠 张云
单位：北京协和医院外科
关键词：
中国临床营养杂志000116摘 要：目的建立偶氮显色鲎试验并测定健康人和临床病人外周静脉血内毒素。 方法利用偶氮显色法原理建立检测微量内毒素技术，测定50例健康成人及51例不同创伤病人外周静脉血的内毒素并对测定方法进行探讨。 结果1.此法检测内毒素可达微量(10-2Eu/ml)，自身变异系数为2.29%(n=5)。2.健康成人外周静脉血内毒素正常值为<0.0330Eu/ml，与国际水准一致。3.病人外周静脉血内毒素水平与创伤程度有关，其血浓度随着创伤程度增加而增加，两者之间呈正相关，R2=0.667。 结论本法灵敏度高，重复性好并可精确定量。
The percentage of bacterial DNA in blood of postoperative surgical patients
WEN Liping ,JIANG Zhuming ,TANG Weisong
(Dept. of Surgery d PUMC Hospital, Beijing 100730)
Abstract：Objective AIM to set up a high sencitive, good reproducible method to detect trace endotoxin and endotoxin in human peripheral venous blood. Methods The method of quantitative determination of endotoxin was established by using Limulus Lysate teat with chromogenie substrate, and the endotoxin levee of peripheral venous blood was determined from 50 healthy subjects and 51 patients. Results 1. Improved Limulus Lysate test with ehromogenic substrate could betect trace endotoxin (10～2Eu/ml) and the CV was 2.29% (n=5). 2. Endotoxin levels of healthy Chinese in peripheral venous blood were below 0. 033Eu/ml. 3. Endotoxin levels were higher in patients sith some major operations, Which were correlated with the severity of stress (R2=0. 665). Conclusions This method is an effective measure ment for the Gram-negative bactexiemio and studies of endotoxemia., 百拇医药
单位：北京协和医院外科
关键词：
中国临床营养杂志000116摘 要：目的建立偶氮显色鲎试验并测定健康人和临床病人外周静脉血内毒素。 方法利用偶氮显色法原理建立检测微量内毒素技术，测定50例健康成人及51例不同创伤病人外周静脉血的内毒素并对测定方法进行探讨。 结果1.此法检测内毒素可达微量(10-2Eu/ml)，自身变异系数为2.29%(n=5)。2.健康成人外周静脉血内毒素正常值为<0.0330Eu/ml，与国际水准一致。3.病人外周静脉血内毒素水平与创伤程度有关，其血浓度随着创伤程度增加而增加，两者之间呈正相关，R2=0.667。 结论本法灵敏度高，重复性好并可精确定量。
The percentage of bacterial DNA in blood of postoperative surgical patients
WEN Liping ,JIANG Zhuming ,TANG Weisong
(Dept. of Surgery d PUMC Hospital, Beijing 100730)
Abstract：Objective AIM to set up a high sencitive, good reproducible method to detect trace endotoxin and endotoxin in human peripheral venous blood. Methods The method of quantitative determination of endotoxin was established by using Limulus Lysate teat with chromogenie substrate, and the endotoxin levee of peripheral venous blood was determined from 50 healthy subjects and 51 patients. Results 1. Improved Limulus Lysate test with ehromogenic substrate could betect trace endotoxin (10～2Eu/ml) and the CV was 2.29% (n=5). 2. Endotoxin levels of healthy Chinese in peripheral venous blood were below 0. 033Eu/ml. 3. Endotoxin levels were higher in patients sith some major operations, Which were correlated with the severity of stress (R2=0. 665). Conclusions This method is an effective measure ment for the Gram-negative bactexiemio and studies of endotoxemia., 百拇医药