扩张时限对皮肤微循环的影响
作者：王友彬 邱玉金 唐胜建 牟少春 张海荣
单位：
关键词：皮肤软组织扩张；微循环；微血管
潍坊医学院000115摘 要：目的实验观察不同扩张时间皮肤微血管系及其血流动力学的变化规律。方法以兔为实验动物，用激光多普勒微循环显微成像、酶组织化学染色并计算机图像分析等形态学研究手段，观测正常及不同扩张时间皮肤血液流速、流量等血流动力学指标及管径、密度等微血管形态学指标。结果对照组、扩张组在微血管直径、血流速度、血流量及微血管密度方面存在显著差异(P<0.01)，直径、流量测量值从第1～3周分别从11.16±1.01um，(11.15±0.85)×105um3增至13.91±1.4um，(15.86±0.93)×105um3，微血管密度在第1～4周从0.022±0.02um2/um2增至0.061±0.003um2/um2，随扩张时间延长上述观测值在出现一过性下降后又呈现同样变化。流速在扩张后增大并维持平均1012.45um/s的较高水平。结论扩张皮肤约3～4周完成一个微血管增生构筑演变周期，达到一次直径、流量及密度最大值。
, 百拇医药
分类号：R331.3+5 文献标识码：A
Skin Microcirculation Change Fellowing Different Expanding Periods
WangYoubin ,Qiu Yujin ,Tang Shengjian
(Plastic and Reconstruction Hospital of Weifang Medical College，Weifang，261042)
Abstract：Objective Experimental study on the changing pattern of the microvasculature and their hemodynamics in expanded skin at different expanding periods.Method Adult rabbits were experimented on.The microvessel diameter,the blood velocity and the blood volume were recorded under the laser doppler microcirculatory imaging system and the microwssel density was measured by enzyme histochemical dying and medical imagine analysis.Result,Compared with the contrast group,the microvessel diameter,the blood velocity,the whole blood volume per second and the microvessel density arised obviously in every expanded group(P<0.01).The recorded values of the microvessel diameter and the blood volume rose seperately from 11.16±1.01 um and(11.15±0.85)×105um3to 13.91±1.4um and(15.86±0.93)×105um3 in the first three-weeks,expanding period.The microvessel density arised from 0.022±0.002um2/m2(the first week)to 0.061±0.003um2/m2(the fourth week).This changing pattern repeated in later expanding period.The blood velocity kept in high level from the first expanding period on.The mean volue was 1012.45±62.01 um/s.Conclusion One angiogenesis phase in skin is about 4 weeks.In the end of this phase,the vessel diameter,the vessel density and the whole blood volume per second reach their highest level of this phase.
, 百拇医药
Keywords：Tissue expansion;Microcirculation;Microvessel
参考文献：
[1]Pang CY,Forrest CR,Neligan PC,et al.Augementation of blood flow in delayed random skin flap in the pig：Effect of legenth of delay periiod and angiogenesis[J].Plast Reconstr Surg,1986,78:68
[2]Sasaki GH,Pang CY.Pathophysiology of skin flap raised on expanded pig skin[J].Plast Reconstr Surg,1984,74:59
, http://www.100md.com
[3]Leighton WD,Russell RC,Marcus DE,et al.Experimental pretransfer expansion of free flap donor site:I:Flap viability and expansion charactoristics[J].Plast Reconstr Surg,1988,82:69
[4]Wardell K,Braverman IM,Silverman DG,et al.Spatial heterogeneity in normal skin perfusion recorded with laser Doppler imaging and flowmetry[J].Microcir Res,1994,48:26
[5]方福德，周吕，丁濂主编.现代医学实验技巧全书[M].北京医科大学、中国协和医科大学联合出版社,1995:137
, http://www.100md.com
[6]Chayen J,Bitensky L,Buter RG.Practical Histochemistry[M].London:Wiley,1973:108
[7]Wickman M,Heden P,Jurell G.Circulatory and metabolic changes in expanded pig skin flaps[J].Plast Reconstr Surg,1991,88:650
[8]Wakui S,Furusato M,Ohshige H,et al.Endothelial pericyte interdigitations in rat subcutaneous disc implanted angiogeneousis[J].Microcirculation,1993,46:19
[9]Jakobsson AE.Angiogenesis induced by mast cell secretion in rat peritoneal connective tissue is a process of three phase[J].Microcir Res,1994,47:252
[10]Iba T,Sumpio BE.Morphological response of human endothelial cells subjected to cyclic strain in vitro[J].Microcir Res,1991,42:245
收稿日期：1999-09-19, http://www.100md.com
单位：
关键词：皮肤软组织扩张；微循环；微血管
潍坊医学院000115摘 要：目的实验观察不同扩张时间皮肤微血管系及其血流动力学的变化规律。方法以兔为实验动物，用激光多普勒微循环显微成像、酶组织化学染色并计算机图像分析等形态学研究手段，观测正常及不同扩张时间皮肤血液流速、流量等血流动力学指标及管径、密度等微血管形态学指标。结果对照组、扩张组在微血管直径、血流速度、血流量及微血管密度方面存在显著差异(P<0.01)，直径、流量测量值从第1～3周分别从11.16±1.01um，(11.15±0.85)×105um3增至13.91±1.4um，(15.86±0.93)×105um3，微血管密度在第1～4周从0.022±0.02um2/um2增至0.061±0.003um2/um2，随扩张时间延长上述观测值在出现一过性下降后又呈现同样变化。流速在扩张后增大并维持平均1012.45um/s的较高水平。结论扩张皮肤约3～4周完成一个微血管增生构筑演变周期，达到一次直径、流量及密度最大值。
, 百拇医药
分类号：R331.3+5 文献标识码：A
Skin Microcirculation Change Fellowing Different Expanding Periods
WangYoubin ,Qiu Yujin ,Tang Shengjian
(Plastic and Reconstruction Hospital of Weifang Medical College，Weifang，261042)
Abstract：Objective Experimental study on the changing pattern of the microvasculature and their hemodynamics in expanded skin at different expanding periods.Method Adult rabbits were experimented on.The microvessel diameter,the blood velocity and the blood volume were recorded under the laser doppler microcirculatory imaging system and the microwssel density was measured by enzyme histochemical dying and medical imagine analysis.Result,Compared with the contrast group,the microvessel diameter,the blood velocity,the whole blood volume per second and the microvessel density arised obviously in every expanded group(P<0.01).The recorded values of the microvessel diameter and the blood volume rose seperately from 11.16±1.01 um and(11.15±0.85)×105um3to 13.91±1.4um and(15.86±0.93)×105um3 in the first three-weeks,expanding period.The microvessel density arised from 0.022±0.002um2/m2(the first week)to 0.061±0.003um2/m2(the fourth week).This changing pattern repeated in later expanding period.The blood velocity kept in high level from the first expanding period on.The mean volue was 1012.45±62.01 um/s.Conclusion One angiogenesis phase in skin is about 4 weeks.In the end of this phase,the vessel diameter,the vessel density and the whole blood volume per second reach their highest level of this phase.
, 百拇医药
Keywords：Tissue expansion;Microcirculation;Microvessel
参考文献：
[1]Pang CY,Forrest CR,Neligan PC,et al.Augementation of blood flow in delayed random skin flap in the pig：Effect of legenth of delay periiod and angiogenesis[J].Plast Reconstr Surg,1986,78:68
[2]Sasaki GH,Pang CY.Pathophysiology of skin flap raised on expanded pig skin[J].Plast Reconstr Surg,1984,74:59
, http://www.100md.com
[3]Leighton WD,Russell RC,Marcus DE,et al.Experimental pretransfer expansion of free flap donor site:I:Flap viability and expansion charactoristics[J].Plast Reconstr Surg,1988,82:69
[4]Wardell K,Braverman IM,Silverman DG,et al.Spatial heterogeneity in normal skin perfusion recorded with laser Doppler imaging and flowmetry[J].Microcir Res,1994,48:26
[5]方福德，周吕，丁濂主编.现代医学实验技巧全书[M].北京医科大学、中国协和医科大学联合出版社,1995:137
, http://www.100md.com
[6]Chayen J,Bitensky L,Buter RG.Practical Histochemistry[M].London:Wiley,1973:108
[7]Wickman M,Heden P,Jurell G.Circulatory and metabolic changes in expanded pig skin flaps[J].Plast Reconstr Surg,1991,88:650
[8]Wakui S,Furusato M,Ohshige H,et al.Endothelial pericyte interdigitations in rat subcutaneous disc implanted angiogeneousis[J].Microcirculation,1993,46:19
[9]Jakobsson AE.Angiogenesis induced by mast cell secretion in rat peritoneal connective tissue is a process of three phase[J].Microcir Res,1994,47:252
[10]Iba T,Sumpio BE.Morphological response of human endothelial cells subjected to cyclic strain in vitro[J].Microcir Res,1991,42:245
收稿日期：1999-09-19, http://www.100md.com