复方丹参注射液对心肌缺氧的保护性观察
作者：王亚利 张式暖 刘克花 魏志新 姜红心
单位：王亚利(潍坊医学院附院 潍坊 261031)；张式暖 魏志新 姜红心(病理学教研室)；刘克花(潍坊医学院附属潍坊市人民医院)
关键词：心肌；丹参；缺氧
潍坊医学院000121摘 要：目的探讨复方丹参注射液对心肌的保护作用。方法建立小鼠缺氧后心肌损伤模型，设治疗组和对照组共25只小鼠，随机分为3组，实验2周后，取新鲜心肌标本，测琥珀酸脱氢酶(SDH)，三磷酸腺苷酶(ATP)，乳酸脱氢酶(LDH)；光镜、电镜观察心肌病理性变化。结果缺氧组SDH，ATP酶减少，LDH升高，心肌间质水肿，心肌细胞线粒体肿胀，肌丝排列紊乱。复方丹参组、心肌酶和心肌超微结构与对照组相比差异均无显著性。结论复方丹参对小鼠心肌缺氧损伤有保护作用。
分类号：R285.5；R965.1 文献标识码：A
Protective Effect of Danshen to the Myocardium with Anoxia
Wang Yali ,ZhangShinuan ,Liu Kehua
(Department of Pedatrics,Hospital of Weifang Medical College，Weifang，261031)
Abstract：Objective To investigate Danshen prenvent the myocardium.Method 25 mice were divided into three groups,the control,the hypoxia and Danshen-treated group.At two weeks after treament,sections of myocardium were made,succinic dehyrogenase(SDH),adenosine trphosphatase(ATP)and lactic dehydrogenase(LDH)were stined.Pathologic changes of myocardium were observed under light microscope and electric microscope.Result In hypoxia group,SDH and ATP decreased,while LDH increased.Mesenchyma of myocardium was edematous.Mitochondrions of myocardial cells enlarged，arrangement of filaments was irregular.There was not a significant difference between Danshen-treated and control group.Conclusion Anoxia causes enzymohistochemical and morphological changes of myocardium.Danshen may prevent the myocardium from damage with anoxia.
Keywords：Myocardium;Danshen;Anoxia
基金项目：山东省潍坊市科委课题(批准号96041)
参考文献：
[1]谢艳玲，张庆松.复方丹参注射液佐治毛细支气管炎[J].中华儿科杂志，1998，(36)8：493
[2]Gumter TE,Pwfeiffer DR.Mechanisms by which mitochondrial transport calcium[J].Am J Physiol,1990,258:755
[3]Minezaki KK,Suleiman MS,Chapman RA.Changer in mitochondrial function induced inisolated guined-pig venticular myocytes by calcium overload[J].Am J Physiol,1994,476:459
[4]张昌颖主编.生物化学[M].第2版，北京：人民卫生出版社，1985：134～136
[5]李才，高长斌，王凡.亚急性克山病心肌线粒体膜结构的形态计量分析[J].中华物理医学杂志，1996，18：105
收稿日期：1998-10-21, http://www.100md.com
单位：王亚利(潍坊医学院附院 潍坊 261031)；张式暖 魏志新 姜红心(病理学教研室)；刘克花(潍坊医学院附属潍坊市人民医院)
关键词：心肌；丹参；缺氧
潍坊医学院000121摘 要：目的探讨复方丹参注射液对心肌的保护作用。方法建立小鼠缺氧后心肌损伤模型，设治疗组和对照组共25只小鼠，随机分为3组，实验2周后，取新鲜心肌标本，测琥珀酸脱氢酶(SDH)，三磷酸腺苷酶(ATP)，乳酸脱氢酶(LDH)；光镜、电镜观察心肌病理性变化。结果缺氧组SDH，ATP酶减少，LDH升高，心肌间质水肿，心肌细胞线粒体肿胀，肌丝排列紊乱。复方丹参组、心肌酶和心肌超微结构与对照组相比差异均无显著性。结论复方丹参对小鼠心肌缺氧损伤有保护作用。
分类号：R285.5；R965.1 文献标识码：A
Protective Effect of Danshen to the Myocardium with Anoxia
Wang Yali ,ZhangShinuan ,Liu Kehua
(Department of Pedatrics,Hospital of Weifang Medical College，Weifang，261031)
Abstract：Objective To investigate Danshen prenvent the myocardium.Method 25 mice were divided into three groups,the control,the hypoxia and Danshen-treated group.At two weeks after treament,sections of myocardium were made,succinic dehyrogenase(SDH),adenosine trphosphatase(ATP)and lactic dehydrogenase(LDH)were stined.Pathologic changes of myocardium were observed under light microscope and electric microscope.Result In hypoxia group,SDH and ATP decreased,while LDH increased.Mesenchyma of myocardium was edematous.Mitochondrions of myocardial cells enlarged，arrangement of filaments was irregular.There was not a significant difference between Danshen-treated and control group.Conclusion Anoxia causes enzymohistochemical and morphological changes of myocardium.Danshen may prevent the myocardium from damage with anoxia.
Keywords：Myocardium;Danshen;Anoxia
基金项目：山东省潍坊市科委课题(批准号96041)
参考文献：
[1]谢艳玲，张庆松.复方丹参注射液佐治毛细支气管炎[J].中华儿科杂志，1998，(36)8：493
[2]Gumter TE,Pwfeiffer DR.Mechanisms by which mitochondrial transport calcium[J].Am J Physiol,1990,258:755
[3]Minezaki KK,Suleiman MS,Chapman RA.Changer in mitochondrial function induced inisolated guined-pig venticular myocytes by calcium overload[J].Am J Physiol,1994,476:459
[4]张昌颖主编.生物化学[M].第2版，北京：人民卫生出版社，1985：134～136
[5]李才，高长斌，王凡.亚急性克山病心肌线粒体膜结构的形态计量分析[J].中华物理医学杂志，1996，18：105
收稿日期：1998-10-21, http://www.100md.com