NGF和bFGF及其受体在金黄地鼠神经管的定位
作者：高英茂 管英俊 吴洪娟 张艳玲
单位：高英茂(山东医科大学组织学与胚胎学教研室 济南 250012)；管英俊 吴洪娟 张艳玲(潍坊医学院 潍坊 261042)
关键词：神经生长因子；成纤维细胞生长因子；受体；神经管；金黄发鼠
解剖科学进展000310摘 要：用免疫组织化学和免疫电镜方法研究了神经生长因子(NGF)和碱性成纤维细胞因子(bFGF)及其受体(TrkA、FGFR)在金黄地鼠神经管发育中的定位分布。结果显示：NGF和bFGF的表达时序不同，但与各自受体的表达时序基本一致，其定位相似。受精后第8d即出现bFGF阳性细胞，NGF于第9d出现，随着胚龄增加，免疫阳性着色逐渐增强，二者均定位于神经细胞胞质、神经管内界膜、外界膜，脊索和脊神经节细胞胞质中，bFGF还分布于神经细胞核。第8d可检测到少量散在的FGFR金标颗粒，TrkA于第9d出现，随着胚胎的发育，金标颗粒逐渐增多，主要定位于细胞膜、胞质的基质、内质网、核膜及胞核中。上述结果表明，NGF、bFGF及其受体在金黄地鼠胚神经管形成和分化中按着一定的时序出现于细胞的一定部位。 The Distribution of NGF, bFGF and their Receptors in Neural Tube of Hamster
, 百拇医药
Guan Yingjun ,Gao Yingmao ,Wu Hongjuan
(Department of Histology and Embryology, Weifang Medical College, Weifang 261042)
Abstract：The localization and distribution o[ NGF, bFGF and their receptors in the developing neural tube of hamster embryo from 8 to 12 days after fertilization were examined with immunohistochemistry and immunoelectron microscopy. The results show that NGF, bFGF and their receptors are expressed in different period of development. The bFGF immunoreactire cells and structure occur on the day 8. NGF immunoreactive cells appear on the day 9. Both NGF and bFGF immunoreactive granules are located in cytoplasm, inner limited membrane and basement memberane of the neural tube, notochord and spinal ganglion. In addition, the bFGF immunoreactivity is also observed in nucleus. The gold-labelling granules of FGF receptor appear on the day 8, but very little in number. NGFR(TrkA) appear on the day 9 and they are located in cell membrane, cytoplasm, endoplasmic reticulum, nuclear membrane and nucleoplasm. The density of gold labelling granules increase gradually as the neural tube developes.
, 百拇医药
Keywords：nerve growth factor; fibroblast growth factor; receptors; neural tube; hamster
参考文献：
[1]周延冲.多肽生长因子基础与临床.北京：中国科技出版社，1992，105～135，209～229
[2]管英俊，高英茂.成纤维细胞生长因子在神经发育中的生物学作用.解剖科学进展，1996，2(3)：229
[3]Mason LJ. The ins and outs of fibroblast growth factor. Cell, 1994, 78: 547
[4]Raffioni S and Bradshaw RA. The receptors for nerve growth factor and other neurotrophins. Annu Rev Biochem. 1993, 62: 825
, 百拇医药
[5]Feinstein SC, Matheson CR, Rade MJ, et al. Immunocytochemical localization of TrkA and TrkB in rat central nervous system. Soc Neurosci Abstr, 1993, 19: 249
[6]Johnsin DE and Willioms LT. Structural and fuctional diversity in the FGF receptor multigene family. Adv Cancer Res, 1993, 60 (1): 1
[7]Stachowiak EK, Mordechai E. Joy A, et al. FGF receptors is a nuclear protein-novel mechanism for bFGF action, Soc Neurosci Ann Meeting. San Diego CA. 1995,1783, 百拇医药
单位：高英茂(山东医科大学组织学与胚胎学教研室 济南 250012)；管英俊 吴洪娟 张艳玲(潍坊医学院 潍坊 261042)
关键词：神经生长因子；成纤维细胞生长因子；受体；神经管；金黄发鼠
解剖科学进展000310摘 要：用免疫组织化学和免疫电镜方法研究了神经生长因子(NGF)和碱性成纤维细胞因子(bFGF)及其受体(TrkA、FGFR)在金黄地鼠神经管发育中的定位分布。结果显示：NGF和bFGF的表达时序不同，但与各自受体的表达时序基本一致，其定位相似。受精后第8d即出现bFGF阳性细胞，NGF于第9d出现，随着胚龄增加，免疫阳性着色逐渐增强，二者均定位于神经细胞胞质、神经管内界膜、外界膜，脊索和脊神经节细胞胞质中，bFGF还分布于神经细胞核。第8d可检测到少量散在的FGFR金标颗粒，TrkA于第9d出现，随着胚胎的发育，金标颗粒逐渐增多，主要定位于细胞膜、胞质的基质、内质网、核膜及胞核中。上述结果表明，NGF、bFGF及其受体在金黄地鼠胚神经管形成和分化中按着一定的时序出现于细胞的一定部位。 The Distribution of NGF, bFGF and their Receptors in Neural Tube of Hamster
, 百拇医药
Guan Yingjun ,Gao Yingmao ,Wu Hongjuan
(Department of Histology and Embryology, Weifang Medical College, Weifang 261042)
Abstract：The localization and distribution o[ NGF, bFGF and their receptors in the developing neural tube of hamster embryo from 8 to 12 days after fertilization were examined with immunohistochemistry and immunoelectron microscopy. The results show that NGF, bFGF and their receptors are expressed in different period of development. The bFGF immunoreactire cells and structure occur on the day 8. NGF immunoreactive cells appear on the day 9. Both NGF and bFGF immunoreactive granules are located in cytoplasm, inner limited membrane and basement memberane of the neural tube, notochord and spinal ganglion. In addition, the bFGF immunoreactivity is also observed in nucleus. The gold-labelling granules of FGF receptor appear on the day 8, but very little in number. NGFR(TrkA) appear on the day 9 and they are located in cell membrane, cytoplasm, endoplasmic reticulum, nuclear membrane and nucleoplasm. The density of gold labelling granules increase gradually as the neural tube developes.
, 百拇医药
Keywords：nerve growth factor; fibroblast growth factor; receptors; neural tube; hamster
参考文献：
[1]周延冲.多肽生长因子基础与临床.北京：中国科技出版社，1992，105～135，209～229
[2]管英俊，高英茂.成纤维细胞生长因子在神经发育中的生物学作用.解剖科学进展，1996，2(3)：229
[3]Mason LJ. The ins and outs of fibroblast growth factor. Cell, 1994, 78: 547
[4]Raffioni S and Bradshaw RA. The receptors for nerve growth factor and other neurotrophins. Annu Rev Biochem. 1993, 62: 825
, 百拇医药
[5]Feinstein SC, Matheson CR, Rade MJ, et al. Immunocytochemical localization of TrkA and TrkB in rat central nervous system. Soc Neurosci Abstr, 1993, 19: 249
[6]Johnsin DE and Willioms LT. Structural and fuctional diversity in the FGF receptor multigene family. Adv Cancer Res, 1993, 60 (1): 1
[7]Stachowiak EK, Mordechai E. Joy A, et al. FGF receptors is a nuclear protein-novel mechanism for bFGF action, Soc Neurosci Ann Meeting. San Diego CA. 1995,1783, 百拇医药