NaOH消蚀法制备人松果体脑砂的扫描电镜观察和化学元素分析
作者：王保芝 樊宇兵 侯广棋 李向印 王丽 王建钦
单位：王保芝 樊宇兵 侯广棋 李向印(河北医科大学人体解剖学教研室，石家庄 050017)；王丽(河北医科大学电子显微镜实验中心)；王建钦(河北医学病理生理学教室)
关键词：脑砂；扫描电镜；元素分析；松果体；人
解剖科学进展000419 摘要 目的 为了探讨人松果体脑砂的结构特征和化学成分。方法 对经NaOH消蚀处理的人松果体内脑砂，进行了扫描电镜观察和化学元素分析。结果 松果体组织块经用NaOH消蚀处理后，其内部的脑砂能被清晰的暴露，并可制得单纯的脑砂样品。松果体脑砂大小不等，常呈桑椹状球形小体；既可存在于松果体的中央区，也可位于松果体的周边部。构成脑砂的化学元素，主要为钙(54.82%)和磷(25.83%)，还有少量银(16.01%)和铁(3.34%)等；钙/磷比值约为2.12/1。结论 扫描电镜观察和化学元素分析的结果提示，脑砂似为矿物质在松果体内的逐渐沉积，更象是一种复杂的时间-数量性矿化，而非简单的年轮样钙化。
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The SEM-observation and Chemical Elements Analysis of Brain Sands in Human Pineal Bodies Prepared with NaOH-Maceration
Wang Baozhi, Wang Li, Wang Jianqin, et al
(Department of Human Anatomy, Hebei Medical University, Shijiazhuang, 050017)
Abstract Objective To explore the structural feature and chemical elements of the brain sands in human pineal bodies. Methods The brain sands of human pineal bodies treated with NaOH-maceration were observed with scanning electron microscope (SEM), and analyzed for its chemical elements. Results The brain sands in pineal bodies treated with NaOH-maceration were clearly exposed, and the pure specimens of brain sands were made up. The pineal brain sands appearing large or small mulbeny-globules, exist within either central region or peripheral position of pineal body. The chemical elements of brain sands are more calcium (54.82%) and phosphorus (25.83%), and less silver (16.01%) and iron (3.34%), Calcium/phosphorus rate was approximately 2.12/1. Conclusion These results indicate that the brain sands is deposited gradually in pineal bodies by minerals, and is a complex time-quantitative mineralization rather than a simplex annual-ring calcification.
, http://www.100md.com
Key words brain sand; SEM; chemical elements analysis; pineal body; human
参考文献
1，宋天保，译.松果体.格氏解剖学[M].第38版，杨琳，高英茂主译.沈阳，辽宁省教育出版社，1999，1888
2，Ohtani O, Ushiki T, Taguchi T, et al. Collagen fibrillar network as skeletal frameworks. A demonstration by cell-maceration/scanning electron microscope method. Arch Histol Cytol, 1988,51(2):249
3，Kodaka T, Moris R, Debari K, et al. Scanning electron microscopy and electron probe microanalysis studies of human pineal concretions. J Electron Microscopy, 1994,43(5):307
, http://www.100md.com
4，Antic S, Rancic G, Avramovic V, et al. Topographical distribution and histochemical characteristics of human pineal acervuli. Acta Anat, 1995,152(4):5
5，王保芝，王建钦，石葛明，等.猴松果体毛细血管和分泌颗粒的超微结构特征及其意义.河北医科大学学报，1999，20(5)：260
6，Mckinley MJ, Oldfiekd BJ. Circumventricular organs. In human microscopic anatomy. Krstic Rv, (ed). Springer-Verlag Barlin heidelberg. Academic Press Inc, 1991,415, http://www.100md.com
单位：王保芝 樊宇兵 侯广棋 李向印(河北医科大学人体解剖学教研室，石家庄 050017)；王丽(河北医科大学电子显微镜实验中心)；王建钦(河北医学病理生理学教室)
关键词：脑砂；扫描电镜；元素分析；松果体；人
解剖科学进展000419 摘要 目的 为了探讨人松果体脑砂的结构特征和化学成分。方法 对经NaOH消蚀处理的人松果体内脑砂，进行了扫描电镜观察和化学元素分析。结果 松果体组织块经用NaOH消蚀处理后，其内部的脑砂能被清晰的暴露，并可制得单纯的脑砂样品。松果体脑砂大小不等，常呈桑椹状球形小体；既可存在于松果体的中央区，也可位于松果体的周边部。构成脑砂的化学元素，主要为钙(54.82%)和磷(25.83%)，还有少量银(16.01%)和铁(3.34%)等；钙/磷比值约为2.12/1。结论 扫描电镜观察和化学元素分析的结果提示，脑砂似为矿物质在松果体内的逐渐沉积，更象是一种复杂的时间-数量性矿化，而非简单的年轮样钙化。
, http://www.100md.com
The SEM-observation and Chemical Elements Analysis of Brain Sands in Human Pineal Bodies Prepared with NaOH-Maceration
Wang Baozhi, Wang Li, Wang Jianqin, et al
(Department of Human Anatomy, Hebei Medical University, Shijiazhuang, 050017)
Abstract Objective To explore the structural feature and chemical elements of the brain sands in human pineal bodies. Methods The brain sands of human pineal bodies treated with NaOH-maceration were observed with scanning electron microscope (SEM), and analyzed for its chemical elements. Results The brain sands in pineal bodies treated with NaOH-maceration were clearly exposed, and the pure specimens of brain sands were made up. The pineal brain sands appearing large or small mulbeny-globules, exist within either central region or peripheral position of pineal body. The chemical elements of brain sands are more calcium (54.82%) and phosphorus (25.83%), and less silver (16.01%) and iron (3.34%), Calcium/phosphorus rate was approximately 2.12/1. Conclusion These results indicate that the brain sands is deposited gradually in pineal bodies by minerals, and is a complex time-quantitative mineralization rather than a simplex annual-ring calcification.
, http://www.100md.com
Key words brain sand; SEM; chemical elements analysis; pineal body; human
参考文献
1，宋天保，译.松果体.格氏解剖学[M].第38版，杨琳，高英茂主译.沈阳，辽宁省教育出版社，1999，1888
2，Ohtani O, Ushiki T, Taguchi T, et al. Collagen fibrillar network as skeletal frameworks. A demonstration by cell-maceration/scanning electron microscope method. Arch Histol Cytol, 1988,51(2):249
3，Kodaka T, Moris R, Debari K, et al. Scanning electron microscopy and electron probe microanalysis studies of human pineal concretions. J Electron Microscopy, 1994,43(5):307
, http://www.100md.com
4，Antic S, Rancic G, Avramovic V, et al. Topographical distribution and histochemical characteristics of human pineal acervuli. Acta Anat, 1995,152(4):5
5，王保芝，王建钦，石葛明，等.猴松果体毛细血管和分泌颗粒的超微结构特征及其意义.河北医科大学学报，1999，20(5)：260
6，Mckinley MJ, Oldfiekd BJ. Circumventricular organs. In human microscopic anatomy. Krstic Rv, (ed). Springer-Verlag Barlin heidelberg. Academic Press Inc, 1991,415, http://www.100md.com