Experimentalobservationoflivingcellsonthesurfaceofintraocularlenses ,1Materialsandmethods,
作者：
单位：
关键词：
白求恩医科大学学报000212〔提 要〕 目的：对比观察后房型人工晶状体植入术后晶状体表面细胞染色前后的数量、形态。方法：20只兔行后房型人工晶状体植入术。分别于术后1，3，7，14和28 d分组取出人工晶状体放入1640细胞培养液于倒置显微镜下观察。结果：术后1～28 d人工晶状体表面有细胞粘附，染色后细胞形态失去立体外观，体积小，细胞数量较染色前明显减少。结论：人工晶状体表面细胞在染色过程中有部分脱落。脱落的细胞数量可以计数比较，但细胞类型无法确定。因此需寻找更精确可靠的仪器及方法，以免造成实验误差。
Experimental observation of living cells on the surface of intraocular lenses
Yu Hongquan,Zhang Yan,Yu Jun
(Department of Ophthalmology,The First Teaching Hospital of
Norman Bethune University of Medical Sciences,Changchun 130021)
〔Abstract〕 Objective:To observe the living cells on the surface of intraocular lenses and to compare the cellular numbers and morphology of prestaining to that of poststaining.Methods:Twenty adult pigment rabbit eyes were given posterior chamber intraocular lens implantation.Lenses were extracted and put into 1640 cell cultural liquid immediately on the 1 st,3 rd,7 th,14 th and 28 th day respectively after operatrion.The samples were observed under inverted phase contrast microscope subsequently.Results:We found cells deposited on the surface of lenses from 1 st to 28 th day after operation.These cells contracted and lost three-demensional appearance while its number was less than that of prestaining obviously.Conclusion:A part of cells remove from the surface of lenses during staining;however,we could count the number but could not affirm cellular types which would make error in cellular classification and counting.The fact suggests that we should discover new accurate and reliable methods to avoid mistakes during experiment. ...... |