不同人大肠癌细胞内质网合成的二硫键异构酶研究
作者：尚德淑 王芸庆 宋今丹
单位：中国医科大学卫生部细胞生物学重点实验室 沈阳 110001
关键词：二硫键异构酶；维四酸；肿瘤细胞侵袭性；肿瘤细胞分化
解剖科学进展000423 摘要 目的：探索PDI与癌细胞生物学特性之间的关系。方法：应用不同侵袭力的大肠癌细胞系CCL-229(高侵袭)和CX-1(低侵袭)，通过流式细胞术分析两者PDI含量的差别，以及经全反式维甲酸(RA)诱导后PDI的变化；间接免疫荧光法观察细胞中PDI的表达情况。结果：CX-1中PDI的含量高于CCL-229；随诱导天数的增加，同一细胞系中PDI含量增加。结论：PDI与癌细胞的侵袭力呈负相关关系；PDI与分化呈正相关关系。
Comparative Study of Protein Disulfide Isomerase Synthesized by ER in Different Cultural Human Colorectal Carcinoma Cell Lines
, http://www.100md.com
Shang Deshu, Wang Yunqing, Song Jindan
(Key Laboratory of Cell Biology, Ministry of Public Health of China, China Medical University, Shenyang 110001, China)
Abstract Objective: Find out the relationship between PDI and biological characteristics of cancer cells. Methods: In this paper, we compared the content of PDI in CCL-229, a high invasive cell line and CX-1, a low invasive cell line of colorectal carcinoma with the method of Flow cytometer (FCM). We also reported the changes in the content of PDI in these two cell lines induced by RA and by applying indirect immuno-florescent method, and observed the expression of PDI in the two cell lines. Results: The content of PDI in CX-1 is higher than that in CCL-229. The content of PDI increases in the two cell lines induced by RA. Conclusion: The relation between PDI and tumor cell invasiveness is negative-relative. The expression of PDI is passive relative to the differentiation of these two cell lines.
, 百拇医药
Key word protein disulfide isomerase; invasive ability; retinoic acid; tumor cell differentiation
国家自然基金重大项目(编号39040005)
参考文献
1，沈关心，周汝麟.现代免疫学实验技术，武汉，湖北科学技术出版社，1998：189
2，宋今丹.整装培养细胞的内质网膜系统研究.电子显微学报，1991，4：355
3，张天泽，徐光炜.肿瘤学，天津，天津科学技术出版社，1996：255
4，宋今丹主编.医学细胞生物学，北京，人民卫生出版社，1992：82
, 百拇医药
5，Wilson R, Lees JF, Bulleid NJ. Protein disulfide isomerase acts as a molecular chaperone during the assembly of procollagen. J Biol chem, 1998,273(6):9637
6.葛常辉，宋今丹.维甲酸诱导大肠癌细胞分化后内质网结构及标志酶G6PA细胞化学的改变.中华物理医学杂志，1995，17(4)：223
7，王秋雨，宋今丹.几种不同进化程度动物细胞蛋白二硫键异构酶活性及含量研究.中国动物科学研究，北京，中国林业出版社，1999，1036
8，Terasaki M, Song JD, Wong JR, et al. Localization of endoplasmic reticulum in living and glutaraldehyde-fixed cells with fluorescent dyes. Cell, 1984,38:101
9，Song JD, Lee C, Lin CS, et al. Electron microscopic studies of the endoplasmic reticulum in whole mount cultured cells fixed with potassium permangnate. J Struc Biol, 1991,107:106, 百拇医药
单位：中国医科大学卫生部细胞生物学重点实验室 沈阳 110001
关键词：二硫键异构酶；维四酸；肿瘤细胞侵袭性；肿瘤细胞分化
解剖科学进展000423 摘要 目的：探索PDI与癌细胞生物学特性之间的关系。方法：应用不同侵袭力的大肠癌细胞系CCL-229(高侵袭)和CX-1(低侵袭)，通过流式细胞术分析两者PDI含量的差别，以及经全反式维甲酸(RA)诱导后PDI的变化；间接免疫荧光法观察细胞中PDI的表达情况。结果：CX-1中PDI的含量高于CCL-229；随诱导天数的增加，同一细胞系中PDI含量增加。结论：PDI与癌细胞的侵袭力呈负相关关系；PDI与分化呈正相关关系。
Comparative Study of Protein Disulfide Isomerase Synthesized by ER in Different Cultural Human Colorectal Carcinoma Cell Lines
, http://www.100md.com
Shang Deshu, Wang Yunqing, Song Jindan
(Key Laboratory of Cell Biology, Ministry of Public Health of China, China Medical University, Shenyang 110001, China)
Abstract Objective: Find out the relationship between PDI and biological characteristics of cancer cells. Methods: In this paper, we compared the content of PDI in CCL-229, a high invasive cell line and CX-1, a low invasive cell line of colorectal carcinoma with the method of Flow cytometer (FCM). We also reported the changes in the content of PDI in these two cell lines induced by RA and by applying indirect immuno-florescent method, and observed the expression of PDI in the two cell lines. Results: The content of PDI in CX-1 is higher than that in CCL-229. The content of PDI increases in the two cell lines induced by RA. Conclusion: The relation between PDI and tumor cell invasiveness is negative-relative. The expression of PDI is passive relative to the differentiation of these two cell lines.
, 百拇医药
Key word protein disulfide isomerase; invasive ability; retinoic acid; tumor cell differentiation
国家自然基金重大项目(编号39040005)
参考文献
1，沈关心，周汝麟.现代免疫学实验技术，武汉，湖北科学技术出版社，1998：189
2，宋今丹.整装培养细胞的内质网膜系统研究.电子显微学报，1991，4：355
3，张天泽，徐光炜.肿瘤学，天津，天津科学技术出版社，1996：255
4，宋今丹主编.医学细胞生物学，北京，人民卫生出版社，1992：82
, 百拇医药
5，Wilson R, Lees JF, Bulleid NJ. Protein disulfide isomerase acts as a molecular chaperone during the assembly of procollagen. J Biol chem, 1998,273(6):9637
6.葛常辉，宋今丹.维甲酸诱导大肠癌细胞分化后内质网结构及标志酶G6PA细胞化学的改变.中华物理医学杂志，1995，17(4)：223
7，王秋雨，宋今丹.几种不同进化程度动物细胞蛋白二硫键异构酶活性及含量研究.中国动物科学研究，北京，中国林业出版社，1999，1036
8，Terasaki M, Song JD, Wong JR, et al. Localization of endoplasmic reticulum in living and glutaraldehyde-fixed cells with fluorescent dyes. Cell, 1984,38:101
9，Song JD, Lee C, Lin CS, et al. Electron microscopic studies of the endoplasmic reticulum in whole mount cultured cells fixed with potassium permangnate. J Struc Biol, 1991,107:106, 百拇医药