CCK与海人酸诱发癫痫敏感性形成的关系
作者：吴钺 张万琴
单位：大连医科大学生理教研室，大连 116027
关键词：海人酸；额叶皮层；海马，癫痫敏感性；CCK
解剖科学进展000317摘 要：用海人酸(Kainic acid，KA)10mg/kg给Sprague-Dawley(SD)大鼠颈部皮下注射，诱发急性癫痫发作，在急性癫痫发作后一周，用阈下剂量(5mg/kg)的KA检测癫痫敏感性。同时分别用原位杂交和免疫组化技术检测并发现癫痫敏感性形成大鼠额叶皮层胆囊收缩素原(PCCK)mRNA明显增加；海马结构中，靠近海马齿状回颗粒细胞(DGCs)基底部锥体样胆囊收缩素(Cholecystoklnin，CCK)免疫反应阳性神经元染色明显增强，但海马门尖部CCK免疫反应阳性神经元明显减少。而癫痫敏感性未形成大鼠未见上述变化，提示上述变化与癫痫敏感性形成有关。
The Relationship Between CCK and Seizure Susceptibility
, 百拇医药
Wu Yue,Zhang Wanqin
(Department of Physiology, Dalian Medical University, Dalian, 116027)
Abstract：Kainic acid (KA) 10mg/kg was subcutaneously injected to Sprague-Dawley rats and induced acute convulsions. A week later, subconvulsive dose of KA (5mg/kg) was used to detect the seizure susceptibility. At the same time, by immunocytochemistry and hybridization, PCCK mRNA was found increased significantly in frontal cortex of susceptible rats. The CCK-IR was enhanced in pyramidal cells near the DGCs of hippocampus and was decreased obviously in the deep region of hilus. These changes were not observed in nonsusceptible rats, which may be relevant to development of seizure susceptibility.
, 百拇医药
Keywords：kainic acid; frontal cortex; hippocampus; seizure susceptibility; CCK
参考文献：
[1]Dodd J, Kelly JS. The actions of cholecystokinin and related peptides on pyramidal neurons of the mammalian hippocampus. Brain Res, 1981, 205: 337
[2]Harro J, Vasar E, Bradwejn J. CCK in animal and human research on anxiety. Tips, 1993, 14: 244
[3]Meyer DK, Widmann R, Sperk G. Increased brain levels of cholecystokinin octapeptide after kainic acid-induced seizures in the rat. Neuroscience Letters, 1986, 69: 208
, 百拇医药
[4]Buckmaster PS, Dudek FE. Neuron loss, granule cell axon reorganization, and functional changes in the dentate gyrna of epileptic kainate-treated rats. J Comp Neurol, 1997, 385: 385
[5]Racine RJ. Modification of seizure activity by electrical stimulation, Ⅱ. Motor seizure. Electroencephalogr Clin Neurophysiol, 1972, 32: 281
[6]Wank SA, Pisegna JR, Weerth AD. Brain and gastrointestinal cholecystokinin receptor family: structure and functional expression. Proc Natl Acad Sci USA, 1992, 89: 8691
, 百拇医药
[7]Shinohara S, Katsuura G, Eigyo M, et al. Inhibitory effect of CCK-8 and ceruletide on glutamate-induced rises in intracellular free calcium concentrations in rat neuron cultures. Brain Res, 1992, 588: 223
[8]Schwarzer C, Williamson JM, Lothman EW, et al. Somatostatin, Neuropeptide Y, Neurokinin B and cholecystokinin immunoreactivity in two chronic models of temporal lobe epilepsy. Neuroscience, 1995, 69: 831
[9]Schwarzer C, Sperk G, Samanin RR, et al. Neuropeptidesimmunoreactivity and their mRNA expression inkindling: functional implications for limbic epileptogenesis. Brain Res Reviews, 1996, 22: 27, 百拇医药
单位：大连医科大学生理教研室，大连 116027
关键词：海人酸；额叶皮层；海马，癫痫敏感性；CCK
解剖科学进展000317摘 要：用海人酸(Kainic acid，KA)10mg/kg给Sprague-Dawley(SD)大鼠颈部皮下注射，诱发急性癫痫发作，在急性癫痫发作后一周，用阈下剂量(5mg/kg)的KA检测癫痫敏感性。同时分别用原位杂交和免疫组化技术检测并发现癫痫敏感性形成大鼠额叶皮层胆囊收缩素原(PCCK)mRNA明显增加；海马结构中，靠近海马齿状回颗粒细胞(DGCs)基底部锥体样胆囊收缩素(Cholecystoklnin，CCK)免疫反应阳性神经元染色明显增强，但海马门尖部CCK免疫反应阳性神经元明显减少。而癫痫敏感性未形成大鼠未见上述变化，提示上述变化与癫痫敏感性形成有关。
The Relationship Between CCK and Seizure Susceptibility
, 百拇医药
Wu Yue,Zhang Wanqin
(Department of Physiology, Dalian Medical University, Dalian, 116027)
Abstract：Kainic acid (KA) 10mg/kg was subcutaneously injected to Sprague-Dawley rats and induced acute convulsions. A week later, subconvulsive dose of KA (5mg/kg) was used to detect the seizure susceptibility. At the same time, by immunocytochemistry and hybridization, PCCK mRNA was found increased significantly in frontal cortex of susceptible rats. The CCK-IR was enhanced in pyramidal cells near the DGCs of hippocampus and was decreased obviously in the deep region of hilus. These changes were not observed in nonsusceptible rats, which may be relevant to development of seizure susceptibility.
, 百拇医药
Keywords：kainic acid; frontal cortex; hippocampus; seizure susceptibility; CCK
参考文献：
[1]Dodd J, Kelly JS. The actions of cholecystokinin and related peptides on pyramidal neurons of the mammalian hippocampus. Brain Res, 1981, 205: 337
[2]Harro J, Vasar E, Bradwejn J. CCK in animal and human research on anxiety. Tips, 1993, 14: 244
[3]Meyer DK, Widmann R, Sperk G. Increased brain levels of cholecystokinin octapeptide after kainic acid-induced seizures in the rat. Neuroscience Letters, 1986, 69: 208
, 百拇医药
[4]Buckmaster PS, Dudek FE. Neuron loss, granule cell axon reorganization, and functional changes in the dentate gyrna of epileptic kainate-treated rats. J Comp Neurol, 1997, 385: 385
[5]Racine RJ. Modification of seizure activity by electrical stimulation, Ⅱ. Motor seizure. Electroencephalogr Clin Neurophysiol, 1972, 32: 281
[6]Wank SA, Pisegna JR, Weerth AD. Brain and gastrointestinal cholecystokinin receptor family: structure and functional expression. Proc Natl Acad Sci USA, 1992, 89: 8691
, 百拇医药
[7]Shinohara S, Katsuura G, Eigyo M, et al. Inhibitory effect of CCK-8 and ceruletide on glutamate-induced rises in intracellular free calcium concentrations in rat neuron cultures. Brain Res, 1992, 588: 223
[8]Schwarzer C, Williamson JM, Lothman EW, et al. Somatostatin, Neuropeptide Y, Neurokinin B and cholecystokinin immunoreactivity in two chronic models of temporal lobe epilepsy. Neuroscience, 1995, 69: 831
[9]Schwarzer C, Sperk G, Samanin RR, et al. Neuropeptidesimmunoreactivity and their mRNA expression inkindling: functional implications for limbic epileptogenesis. Brain Res Reviews, 1996, 22: 27, 百拇医药