自发性高血压鼠脑底动脉血管活性肠多肽能神经纤维的起源
作者：林雪群 石嘉庆 杨刚 邵立健
单位：江西医学院解剖学教研室，南昌 330006
关键词：脑动脉；高血压；血管活性肠多肽；神经
解剖科学进展000325摘 要：应用免疫组织化学ABC法和神经节切除术，观察了20只自发性高血压鼠(分手术Ⅰ、Ⅱ、Ⅲ组和对照组)脑底动脉血管活性肠多肽能神经纤维的起源。结果显示，对照组脑底血管的大脑前动脉、大脑中动脉、大脑后动脉和基底动脉壁上均可见棕褐争的免疫反应阳性纤维，纤维似细曲线状，呈网状走行。手术Ⅰ组作一侧蝶腭神经节切除术，双侧脑底动脉主要分支的阳性纤维明显减少；手术Ⅱ组作一侧耳神经节切除术，双侧大脑后动脉和基底动脉壁上的阳性纤维密度减少；手术Ⅲ组作一侧颈上神经节切除术，基底动脉的阳性纤维减少。结果表明：自发性高血压鼠一侧脑底动脉主要分支的血管活性肠多肽能神经纤维起源于双侧蝶腭神经节、耳神经节和颈上神经节。提示血管活性肠多肽能神经可能在高血压鼠的脑血管运动和高血压的发生和发展方面起着重要的作用。
, 百拇医药
Origin of the Vasoactive Intestinal Polypeptidergic Nerve on Cerebral Vessels in Spontaneously Hypertensive Rats
Lin Xuequn ,Shi Jiaqing
Abstract：Abstract Origin of the vasoactive intestinal polypeptidergic nerves on cerebral vessels in 20 spontaneously hypertensive rats (SHR) (divided into operating Ⅰ、Ⅱ、Ⅲ、groups and control group) were studied with ganglionectomy and ABC immunoperoxidase technique, employing vasoactive intestinal polypeptide (VIP) as primary antibody. The immunoreactive positive fibers with the brown lineal structures were observed on the anterior cerebral artery (ACA). middle cerebral artery (MCA)、posterior cerebral artery (PCA) and basilar artery (BA) in control groups. Density on bilateral ACA、MCA、PCA and BA obvious decreased. In operating Ⅱ group with unilateral otic ganglionectomy, the density of the positive fibers on bilateral PCA and BA decreased. In operating Ⅲ group with unilateral superior cervical ganglionectomy, the positive fiber’s density on BA decreased. It is suggested that vasoactive intestinal polypeptidergic nerve fibers on unilateral cerebral vessels of the SHR originate from bilateral sphenopalatine, otic, superior cervical ganglion, and the vasoactive intesinal polypeptidergic nerves play an important role in the maintenance and development of the hypertension in SHR.
, 百拇医药
Keywords：cerebral vessel; hypertension; vasoactive intestinal polypeptidergic; nerve
参考文献：
[1]林雪群，石嘉庆，程上穆，自发性高血压鼠脑血管肾上腺素能神经分布的形态学观察.神经解剖学杂志，1998, 14(4): 314
[2]林雪群，程上穆，石嘉庆，等.自发性高血压鼠脑底动脉肾上腺素能神经起源的观察.中国组织化学与细胞化学杂志，1999, 8(2): 181
[3]Dhital KK, Gerli R, Lincoln, et al. Increased density of perivascular nerves to the major cerebral vessels of the spontaneously hypertensive rat: differential changes in noradrenaline and neuropeptide Y during development. Brain Res, 1988, 444(1): 33
, 百拇医药
[4]Hsu SM, Raine L, Fanger, et al. Use of Avidin-Biodin-Peroxidase Comples(ABC) in immunoperoxidase techniques. J Histochem Cytochem, 1981, 29: 577
[5]Lee RM, Nagahama M, Mckenzie R, et al. Peptide-containing nerves around blood vessels of stroke-prone spontaneously hypertinsive rats. Hypertension, 1988, 11(2 Pt2): I 117
[6]Kawamura K, Takebaysahi S. Perivaseular innervation of the cerebral arteries in spontaneously hypertensive rsts-an immunohistochemieal study. Angiology, 1991, 42(2); 123
, http://www.100md.com
[7]Koketsu N, Moskowitz MA, Kontos HA, et al. Chronic parasympathetic sectioning decreases regional cerebral blood flow during hemorrhagic hypotension and increases infarct size after middle cerebral artery occlusion in spontaneously hypertensive rats. J Cereb Blood Flow Metab, 1992, 12(4): 613
[8]Lee TJ, Saito A. Altered cerebral vessel innervation in spontaneously hypertensive rats. Cire Res, 1984, 55(3): 392
[9]Zhong KS, Gu ZZ. Peptidergic regulation in the cerebral vessels. Sheng Li Ko Hsueh Chin Chan, 1993, 24(4): 330, 百拇医药
单位：江西医学院解剖学教研室，南昌 330006
关键词：脑动脉；高血压；血管活性肠多肽；神经
解剖科学进展000325摘 要：应用免疫组织化学ABC法和神经节切除术，观察了20只自发性高血压鼠(分手术Ⅰ、Ⅱ、Ⅲ组和对照组)脑底动脉血管活性肠多肽能神经纤维的起源。结果显示，对照组脑底血管的大脑前动脉、大脑中动脉、大脑后动脉和基底动脉壁上均可见棕褐争的免疫反应阳性纤维，纤维似细曲线状，呈网状走行。手术Ⅰ组作一侧蝶腭神经节切除术，双侧脑底动脉主要分支的阳性纤维明显减少；手术Ⅱ组作一侧耳神经节切除术，双侧大脑后动脉和基底动脉壁上的阳性纤维密度减少；手术Ⅲ组作一侧颈上神经节切除术，基底动脉的阳性纤维减少。结果表明：自发性高血压鼠一侧脑底动脉主要分支的血管活性肠多肽能神经纤维起源于双侧蝶腭神经节、耳神经节和颈上神经节。提示血管活性肠多肽能神经可能在高血压鼠的脑血管运动和高血压的发生和发展方面起着重要的作用。
, 百拇医药
Origin of the Vasoactive Intestinal Polypeptidergic Nerve on Cerebral Vessels in Spontaneously Hypertensive Rats
Lin Xuequn ,Shi Jiaqing
Abstract：Abstract Origin of the vasoactive intestinal polypeptidergic nerves on cerebral vessels in 20 spontaneously hypertensive rats (SHR) (divided into operating Ⅰ、Ⅱ、Ⅲ、groups and control group) were studied with ganglionectomy and ABC immunoperoxidase technique, employing vasoactive intestinal polypeptide (VIP) as primary antibody. The immunoreactive positive fibers with the brown lineal structures were observed on the anterior cerebral artery (ACA). middle cerebral artery (MCA)、posterior cerebral artery (PCA) and basilar artery (BA) in control groups. Density on bilateral ACA、MCA、PCA and BA obvious decreased. In operating Ⅱ group with unilateral otic ganglionectomy, the density of the positive fibers on bilateral PCA and BA decreased. In operating Ⅲ group with unilateral superior cervical ganglionectomy, the positive fiber’s density on BA decreased. It is suggested that vasoactive intestinal polypeptidergic nerve fibers on unilateral cerebral vessels of the SHR originate from bilateral sphenopalatine, otic, superior cervical ganglion, and the vasoactive intesinal polypeptidergic nerves play an important role in the maintenance and development of the hypertension in SHR.
, 百拇医药
Keywords：cerebral vessel; hypertension; vasoactive intestinal polypeptidergic; nerve
参考文献：
[1]林雪群，石嘉庆，程上穆，自发性高血压鼠脑血管肾上腺素能神经分布的形态学观察.神经解剖学杂志，1998, 14(4): 314
[2]林雪群，程上穆，石嘉庆，等.自发性高血压鼠脑底动脉肾上腺素能神经起源的观察.中国组织化学与细胞化学杂志，1999, 8(2): 181
[3]Dhital KK, Gerli R, Lincoln, et al. Increased density of perivascular nerves to the major cerebral vessels of the spontaneously hypertensive rat: differential changes in noradrenaline and neuropeptide Y during development. Brain Res, 1988, 444(1): 33
, 百拇医药
[4]Hsu SM, Raine L, Fanger, et al. Use of Avidin-Biodin-Peroxidase Comples(ABC) in immunoperoxidase techniques. J Histochem Cytochem, 1981, 29: 577
[5]Lee RM, Nagahama M, Mckenzie R, et al. Peptide-containing nerves around blood vessels of stroke-prone spontaneously hypertinsive rats. Hypertension, 1988, 11(2 Pt2): I 117
[6]Kawamura K, Takebaysahi S. Perivaseular innervation of the cerebral arteries in spontaneously hypertensive rsts-an immunohistochemieal study. Angiology, 1991, 42(2); 123
, http://www.100md.com
[7]Koketsu N, Moskowitz MA, Kontos HA, et al. Chronic parasympathetic sectioning decreases regional cerebral blood flow during hemorrhagic hypotension and increases infarct size after middle cerebral artery occlusion in spontaneously hypertensive rats. J Cereb Blood Flow Metab, 1992, 12(4): 613
[8]Lee TJ, Saito A. Altered cerebral vessel innervation in spontaneously hypertensive rats. Cire Res, 1984, 55(3): 392
[9]Zhong KS, Gu ZZ. Peptidergic regulation in the cerebral vessels. Sheng Li Ko Hsueh Chin Chan, 1993, 24(4): 330, 百拇医药