人抗动物抗体及其对免疫分析的干扰
作者：杨朝国\, http://www.100md.com
单位：四川省卫生管理干部学院，四川 成都 610041\, http://www.100md.com
关键词：人抗动物抗体；免疫分析；干扰\, http://www.100md.com
免疫学杂志010121 摘 要：人抗动物抗体可引起临床不良反应，干扰动物源性抗体制剂治疗或成像，干扰免疫分析而引起假性结果。临床上可通过应用免疫抑制剂治疗和人源性抗体、聚乙二醇处理的抗体或抗体Fab段制剂等方法防止人抗动物抗体应答形成；样品预处理或分析技术的改进可消除人抗动物抗体对免疫分析的干扰。目前已设计出了多种分析技术检测人抗动物抗体，并形成了检测试剂盒，甚至床边检验试剂。\, http://www.100md.com
分类号：R392.11 文献标识码：A\, http://www.100md.com
文章编号：1000-8861(2001)01-0069-03\, http://www.100md.com
Human anti-animal antibody and the interference in immunological assays\, http://www.100md.com
YANG Zhao-guo(Sichuan Continuing Education College of Medical Sciences，Chengdu 640041，China)\, http://www.100md.com
Abstract：Human anti-animal antibody response may result in some adverse reaction interfere with antibodies from animal therapy or imaging，and cause both false-positive and false-negative test results in immunoassays Strategies to prevent the development of human anti-animal antibody responses include immunosuppressant therapy and the use of humanized，polyethylene glycolytated antibodies，or Fab fragment of antibody agents.Sample pretreatments or assay redesign can eliminate immunoassay interference caused by human anti-animal antibodies.Currently，several assays have been designed to detect human anti-animal antibodies.There are the assays available in kit form，even a simple point-of care test.
Keywords：Human Anti-animal Antibody；Immunological Assay；Interference8l00)u0, 百拇医药
作者简介：杨朝国(1964-)，男，四川南充市人，副教授，大专，主要从事分子免疫学研究。8l00)u0, 百拇医药
参考文献：8l00)u0, 百拇医药
[1]Kohse KP，Wisser H. Antibodies as a source of analytical errors[J].J Clin Biochem，1990，28(1)：881-892.8l00)u0, 百拇医药
[2]Nores GA，Dennis RD，Helling F，et al. Human heterophile antibodies recognizing epitopes present on insect glycolipids[J].J Biochem，1991，110(1)：1-8.8l00)u0, 百拇医药
[3]Kuus-Reichel K，Grauer LS，Karavodin LM，et al. Will immunogenicity limit the use，efficacy and future development of therapeutic monoclonal antibodies?[J]Clin Diagn Lab Immunol，1994，1(2)：365-372.8l00)u0, 百拇医药
[4]Lind P，Lechner P，Arian-Schad K，et al. Anti-carcinoembryonic antigen immunoscintigraphy(technetium-99 m-monoclonal antibody BW431/26)and serum CEA levels in patients with suspected primary and recurrent colorectal carcinoma[J].J Nucl Med，1991，32(10)：1 319-1 325.8l00)u0, 百拇医药
[5]Frodin JE，Lefvert AK，Mellsted H. The clinical significance of HAMA in patients treated with mouse monoclonal antibodies[J].Cell Biophys，1992，21(1)：153-165.
[6]Thompson RJ，Jachson AP，Langiois N. Circulating antibodies to mouse monoclonal immunoglobulins in normal subjects-incidence，species specificity，and effects on a two-site assay for creatine kinase-MB isoenzyme[J].Clin Chem，1986，32(3)：476-481.!{%)f1[, 百拇医药
[7]Sampson M，Ruddel M，Zweig M，et al. Falsely high concentration of serum lutropin measured with the Abbot IMX[J].Clin Chem，1994，40(11)：1 976-1 977.!{%)f1[, 百拇医药
[8]Moseley KR，Knapp RC，Haisma HJ. An assay for the detection of human anti-murine immunoglobulins in the presence of CA125 antigen[J].J Immunol Methods，1988，106(1)：1-6.!{%)f1[, 百拇医药
[9]Shama SK，Bagshawe KD，Melton RG，et al. Human immune response to monoclonal antibodyenzyme conjugates in ADEPT pilot clinical trial[J].Cell Biophys，1992，21(1)：109-120.!{%)f1[, 百拇医药
[10]Baum RP，Niesen A，Hertel A，et al. Activating anti-idiotypic human anti-mouse antibodes for immunotherapy of ovarian carcinoma[J].Cancer，1994，73(suppl)：1 121-1 125.!{%)f1[, 百拇医药
[11]Murray JL，Pietersz GA，Teh JG，et al. Phase Ⅰ trial of murine monoclonal antibody 14G2a administered by prolonged intravenous infusion in patients with neuroectodermal tumors[J].J Clin Oncol，1994，12(2)：184-193.
[12]Torres G，Bema L，Estorch M，et al. Pre-existing human anti-murine antibodies and the effect of immune complexes on the outcome of immunoscintigraphy[J].Clin Nucl Med，1993，18(3)：471-481.z0, 百拇医药
[13]Wieden PL，Wolf SB，Breitz HB，et al. Human anti-mouse antibody suppression with cyclosprin A[J].Cancer，1994，73(7)：1 093-1 097.z0, 百拇医药
[14]Delgado C，Pedley RB，Herraez A，et al. Enhanced tumor specificity of an anti-carcinoembryonic antigen Fab' fragment by poly(ethyleneglycol)(PEG)modification[J].Br J Cancer，1996，73(2)：175-182.z0, 百拇医药
[15]Tjandra JJ，Ramzdi L，Mckenzie IFC. Development of human anti-murine antibody(HAMA) response in patients[J]. Immunol Cell Biol，1990，68(3)：367-376.z0, 百拇医药
[16]Hama Survey Group.Interlaboratory survey of methods for measuring human anti-mouse antibodies[J].Clin Chem，1992，38(2)：172-173.z0, 百拇医药
[17]Turpeinen U，Lethtovirta P，Stenman UH. CA125 determined by three methods in samples from patients with human anti-mouse antibodies(HAMA)[J].Clin Chem，1995，41(10)：1 667-1 669.z0, 百拇医药
[18]Kricka LJ，Schmerfeld-Pruss D，Senior M，et al. Interference by human anti-mouse antibody in two-site immunoassays[J].Clin Chem，1990，36(6)：892-894.
[19]Fitzmaurice TF，Brown C，Rifai N，et al. False increase of cardiac troponin Ⅰ with heterophillic antibodies[J].Clin Chem，1998，44(12)：2 212-2 214.(|5u, 百拇医药
[20]Reinsberg J，Schmolling J，Ackermann D. A simple and sensitive assay for determination of human anti-idiotypic anti-B72.3 antibodies，which is not affected by the presence of tumour-associated glycoprotein 72[J].Eur J Clin Chem Clin Biochem，1996，34(2)：237-244.(|5u, 百拇医药
[21]Sosolik RC，Hitchcock CL，Beker WJ. Heterophillic antibodies produce spuriously elevated concentration of the MB isoenzyme of creatine kinase in a selected patient population[J].Am J Clin Pathol，1997，107(3)：506-510.(|5u, 百拇医药
[22]Hasholzner U，Stieber P，Meier W，et al. Value of HAMA-determination in clinical practice[J].Anticancer Res，1997，17(9)：3 055-3 058.(|5u, 百拇医药
[23]Madry N，Auerbach B，Schelp C. Measures to overcome HAMA interferences in immunoassays[J].Anticancer Res，1997，17(9)：2 883-2 886.(|5u, 百拇医药
收稿日期：2000年1月27日(|5u, 百拇医药
修稿日期：2000年7月10日(|5u, 百拇医药
出版日期：2001年1月15日(杨朝国)
单位：四川省卫生管理干部学院，四川 成都 610041\, http://www.100md.com
关键词：人抗动物抗体；免疫分析；干扰\, http://www.100md.com
免疫学杂志010121 摘 要：人抗动物抗体可引起临床不良反应，干扰动物源性抗体制剂治疗或成像，干扰免疫分析而引起假性结果。临床上可通过应用免疫抑制剂治疗和人源性抗体、聚乙二醇处理的抗体或抗体Fab段制剂等方法防止人抗动物抗体应答形成；样品预处理或分析技术的改进可消除人抗动物抗体对免疫分析的干扰。目前已设计出了多种分析技术检测人抗动物抗体，并形成了检测试剂盒，甚至床边检验试剂。\, http://www.100md.com
分类号：R392.11 文献标识码：A\, http://www.100md.com
文章编号：1000-8861(2001)01-0069-03\, http://www.100md.com
Human anti-animal antibody and the interference in immunological assays\, http://www.100md.com
YANG Zhao-guo(Sichuan Continuing Education College of Medical Sciences，Chengdu 640041，China)\, http://www.100md.com
Abstract：Human anti-animal antibody response may result in some adverse reaction interfere with antibodies from animal therapy or imaging，and cause both false-positive and false-negative test results in immunoassays Strategies to prevent the development of human anti-animal antibody responses include immunosuppressant therapy and the use of humanized，polyethylene glycolytated antibodies，or Fab fragment of antibody agents.Sample pretreatments or assay redesign can eliminate immunoassay interference caused by human anti-animal antibodies.Currently，several assays have been designed to detect human anti-animal antibodies.There are the assays available in kit form，even a simple point-of care test.
Keywords：Human Anti-animal Antibody；Immunological Assay；Interference8l00)u0, 百拇医药
作者简介：杨朝国(1964-)，男，四川南充市人，副教授，大专，主要从事分子免疫学研究。8l00)u0, 百拇医药
参考文献：8l00)u0, 百拇医药
[1]Kohse KP，Wisser H. Antibodies as a source of analytical errors[J].J Clin Biochem，1990，28(1)：881-892.8l00)u0, 百拇医药
[2]Nores GA，Dennis RD，Helling F，et al. Human heterophile antibodies recognizing epitopes present on insect glycolipids[J].J Biochem，1991，110(1)：1-8.8l00)u0, 百拇医药
[3]Kuus-Reichel K，Grauer LS，Karavodin LM，et al. Will immunogenicity limit the use，efficacy and future development of therapeutic monoclonal antibodies?[J]Clin Diagn Lab Immunol，1994，1(2)：365-372.8l00)u0, 百拇医药
[4]Lind P，Lechner P，Arian-Schad K，et al. Anti-carcinoembryonic antigen immunoscintigraphy(technetium-99 m-monoclonal antibody BW431/26)and serum CEA levels in patients with suspected primary and recurrent colorectal carcinoma[J].J Nucl Med，1991，32(10)：1 319-1 325.8l00)u0, 百拇医药
[5]Frodin JE，Lefvert AK，Mellsted H. The clinical significance of HAMA in patients treated with mouse monoclonal antibodies[J].Cell Biophys，1992，21(1)：153-165.
[6]Thompson RJ，Jachson AP，Langiois N. Circulating antibodies to mouse monoclonal immunoglobulins in normal subjects-incidence，species specificity，and effects on a two-site assay for creatine kinase-MB isoenzyme[J].Clin Chem，1986，32(3)：476-481.!{%)f1[, 百拇医药
[7]Sampson M，Ruddel M，Zweig M，et al. Falsely high concentration of serum lutropin measured with the Abbot IMX[J].Clin Chem，1994，40(11)：1 976-1 977.!{%)f1[, 百拇医药
[8]Moseley KR，Knapp RC，Haisma HJ. An assay for the detection of human anti-murine immunoglobulins in the presence of CA125 antigen[J].J Immunol Methods，1988，106(1)：1-6.!{%)f1[, 百拇医药
[9]Shama SK，Bagshawe KD，Melton RG，et al. Human immune response to monoclonal antibodyenzyme conjugates in ADEPT pilot clinical trial[J].Cell Biophys，1992，21(1)：109-120.!{%)f1[, 百拇医药
[10]Baum RP，Niesen A，Hertel A，et al. Activating anti-idiotypic human anti-mouse antibodes for immunotherapy of ovarian carcinoma[J].Cancer，1994，73(suppl)：1 121-1 125.!{%)f1[, 百拇医药
[11]Murray JL，Pietersz GA，Teh JG，et al. Phase Ⅰ trial of murine monoclonal antibody 14G2a administered by prolonged intravenous infusion in patients with neuroectodermal tumors[J].J Clin Oncol，1994，12(2)：184-193.
[12]Torres G，Bema L，Estorch M，et al. Pre-existing human anti-murine antibodies and the effect of immune complexes on the outcome of immunoscintigraphy[J].Clin Nucl Med，1993，18(3)：471-481.z0, 百拇医药
[13]Wieden PL，Wolf SB，Breitz HB，et al. Human anti-mouse antibody suppression with cyclosprin A[J].Cancer，1994，73(7)：1 093-1 097.z0, 百拇医药
[14]Delgado C，Pedley RB，Herraez A，et al. Enhanced tumor specificity of an anti-carcinoembryonic antigen Fab' fragment by poly(ethyleneglycol)(PEG)modification[J].Br J Cancer，1996，73(2)：175-182.z0, 百拇医药
[15]Tjandra JJ，Ramzdi L，Mckenzie IFC. Development of human anti-murine antibody(HAMA) response in patients[J]. Immunol Cell Biol，1990，68(3)：367-376.z0, 百拇医药
[16]Hama Survey Group.Interlaboratory survey of methods for measuring human anti-mouse antibodies[J].Clin Chem，1992，38(2)：172-173.z0, 百拇医药
[17]Turpeinen U，Lethtovirta P，Stenman UH. CA125 determined by three methods in samples from patients with human anti-mouse antibodies(HAMA)[J].Clin Chem，1995，41(10)：1 667-1 669.z0, 百拇医药
[18]Kricka LJ，Schmerfeld-Pruss D，Senior M，et al. Interference by human anti-mouse antibody in two-site immunoassays[J].Clin Chem，1990，36(6)：892-894.
[19]Fitzmaurice TF，Brown C，Rifai N，et al. False increase of cardiac troponin Ⅰ with heterophillic antibodies[J].Clin Chem，1998，44(12)：2 212-2 214.(|5u, 百拇医药
[20]Reinsberg J，Schmolling J，Ackermann D. A simple and sensitive assay for determination of human anti-idiotypic anti-B72.3 antibodies，which is not affected by the presence of tumour-associated glycoprotein 72[J].Eur J Clin Chem Clin Biochem，1996，34(2)：237-244.(|5u, 百拇医药
[21]Sosolik RC，Hitchcock CL，Beker WJ. Heterophillic antibodies produce spuriously elevated concentration of the MB isoenzyme of creatine kinase in a selected patient population[J].Am J Clin Pathol，1997，107(3)：506-510.(|5u, 百拇医药
[22]Hasholzner U，Stieber P，Meier W，et al. Value of HAMA-determination in clinical practice[J].Anticancer Res，1997，17(9)：3 055-3 058.(|5u, 百拇医药
[23]Madry N，Auerbach B，Schelp C. Measures to overcome HAMA interferences in immunoassays[J].Anticancer Res，1997，17(9)：2 883-2 886.(|5u, 百拇医药
收稿日期：2000年1月27日(|5u, 百拇医药
修稿日期：2000年7月10日(|5u, 百拇医药
出版日期：2001年1月15日(杨朝国)