放线菌酮诱导大鼠肠系膜淋巴结淋巴细胞凋亡
作者：霍霞 徐锡金 朴英杰 黄行许
单位：霍霞(汕头大学医学院中心实验室)；徐锡金(组胚教教研室 汕头 515031)；朴英杰 黄行许(第一军医大学中心实验室，广州 510515)
关键词：放线菌酮；淋巴细胞；细胞凋亡；原位末端标记；电镜
解剖科学进展000416 摘要 本实验利用放线菌酮建造细胞凋亡动物模型，用原位末端标记和电镜方法观察了大鼠肠系膜淋巴结淋巴细胞凋亡的形态学变化。结果显示放线菌酮处理4 h后，肠系膜淋巴结部分淋巴细胞处于凋亡状态，主要表现为细胞核凝聚, 染色质边集，可见线粒体、内质网增殖, 自噬体和凋亡小体形成。结果表明放线菌酮主要诱发肠系膜淋巴结生发中心的B淋巴细胞凋亡，线粒体、内质网的增殖是细胞凋亡的一种主动行为。
Cycloheximide-induced Apoptosis of Lymphocyte in Mesenteric Lymph Nodes
, 百拇医药
Huo Xia,Piao Yingjie, Huang Xinxu
(Shantou University Medical College, Shantou, 515031)
Xu Xijin
(Central Lab, Dept. of Hist-Embryology
Abstract Using the SD rats as an experimental model of apoptosis in lymphocytes of the mesenteric lymph nodes with cycloheximide, the structure of lymphocyte were observed with in situ end labeling and electron microscope technique. The results showed that many of the lymphocytes displayed the morphology of apoptosis after 4 hours treatment with cycloheximide with shrinkage of nuclear pyknosis, or chromatin condensation and margination, mitochondria and endoplastic reticula proliferation, autophagosomes and apoptotic bodies formation. Our present study demonstrated that cycloheximide mainly induced apoptosis of B lymphocyte in germinal center of mesenteric lymph nodes. The phenomenon of mitochondria and endoplastic reticula proliferation was an active process of apoptosis.
, 百拇医药
Key Words cycloheximide； lymphocyte； apoptosis； in situ end labeling； electron microscope
参考文献
1，Wirleitner B, Baier-Bitterlich G, Bock G. 7,8-Dihydroneopterin-induced apoptosis in Jurkat T lymphocytes: a comparison with anti-Fas-and hydrogen peroxide-mediated cell death. Biochem Pharmacol, 1998,56(9):1181
2，谭小华，张亚历，姜泊，等. 常规组织切片凋亡细胞原位末端标记方法. 细胞生物学杂志，1997，19(1)：48
, 百拇医药
3，Schwartz LM. The role of cell death genes during development. Bio Essays, 1991,13:389
4，Ledda-Columbano GM, Coni P, Faa G,et al. Rapid induction of apoptosis in rat liver by cycloheximide. Am J Pathol, 1992,140:545
5，Ijiri K, Potten S. Further studies on the response of intestinal crypt cells of different hierarchical status to eighteen different cytotoxic drugs. Br J Cancer, 1987,55:113
6，Cohen JJ, Duke RC. Apoptosis and programmed cell death in immunity. Annu Rev Immunol, 1992,10:267
7，朴英杰，董武，霍霞，等. γ射线照射大鼠脾脏淋巴细胞凋亡及其和巨噬细胞的关系. 电子显微学报，1996，15(2)：335
8，Piao YJ, Liu LP, Huo X, et al. Radiation-induced autophagic apoptosis of rat splenic lymphocytes. Acta Histochem Cytochem，1997，30(5-6)：629, 百拇医药
单位：霍霞(汕头大学医学院中心实验室)；徐锡金(组胚教教研室 汕头 515031)；朴英杰 黄行许(第一军医大学中心实验室，广州 510515)
关键词：放线菌酮；淋巴细胞；细胞凋亡；原位末端标记；电镜
解剖科学进展000416 摘要 本实验利用放线菌酮建造细胞凋亡动物模型，用原位末端标记和电镜方法观察了大鼠肠系膜淋巴结淋巴细胞凋亡的形态学变化。结果显示放线菌酮处理4 h后，肠系膜淋巴结部分淋巴细胞处于凋亡状态，主要表现为细胞核凝聚, 染色质边集，可见线粒体、内质网增殖, 自噬体和凋亡小体形成。结果表明放线菌酮主要诱发肠系膜淋巴结生发中心的B淋巴细胞凋亡，线粒体、内质网的增殖是细胞凋亡的一种主动行为。
Cycloheximide-induced Apoptosis of Lymphocyte in Mesenteric Lymph Nodes
, 百拇医药
Huo Xia,Piao Yingjie, Huang Xinxu
(Shantou University Medical College, Shantou, 515031)
Xu Xijin
(Central Lab, Dept. of Hist-Embryology
Abstract Using the SD rats as an experimental model of apoptosis in lymphocytes of the mesenteric lymph nodes with cycloheximide, the structure of lymphocyte were observed with in situ end labeling and electron microscope technique. The results showed that many of the lymphocytes displayed the morphology of apoptosis after 4 hours treatment with cycloheximide with shrinkage of nuclear pyknosis, or chromatin condensation and margination, mitochondria and endoplastic reticula proliferation, autophagosomes and apoptotic bodies formation. Our present study demonstrated that cycloheximide mainly induced apoptosis of B lymphocyte in germinal center of mesenteric lymph nodes. The phenomenon of mitochondria and endoplastic reticula proliferation was an active process of apoptosis.
, 百拇医药
Key Words cycloheximide； lymphocyte； apoptosis； in situ end labeling； electron microscope
参考文献
1，Wirleitner B, Baier-Bitterlich G, Bock G. 7,8-Dihydroneopterin-induced apoptosis in Jurkat T lymphocytes: a comparison with anti-Fas-and hydrogen peroxide-mediated cell death. Biochem Pharmacol, 1998,56(9):1181
2，谭小华，张亚历，姜泊，等. 常规组织切片凋亡细胞原位末端标记方法. 细胞生物学杂志，1997，19(1)：48
, 百拇医药
3，Schwartz LM. The role of cell death genes during development. Bio Essays, 1991,13:389
4，Ledda-Columbano GM, Coni P, Faa G,et al. Rapid induction of apoptosis in rat liver by cycloheximide. Am J Pathol, 1992,140:545
5，Ijiri K, Potten S. Further studies on the response of intestinal crypt cells of different hierarchical status to eighteen different cytotoxic drugs. Br J Cancer, 1987,55:113
6，Cohen JJ, Duke RC. Apoptosis and programmed cell death in immunity. Annu Rev Immunol, 1992,10:267
7，朴英杰，董武，霍霞，等. γ射线照射大鼠脾脏淋巴细胞凋亡及其和巨噬细胞的关系. 电子显微学报，1996，15(2)：335
8，Piao YJ, Liu LP, Huo X, et al. Radiation-induced autophagic apoptosis of rat splenic lymphocytes. Acta Histochem Cytochem，1997，30(5-6)：629, 百拇医药