【摘要】 目的 探索雌激素刺激乳腺癌病变过程中染色体缩合调控子2(RCC2)的作用及机制。方法 收集乳腺标本，用蛋白免疫印迹法检测RCC2蛋白表达水平；分别用雌激素(雌二醇)、RCC2 小干扰RNA(siRNA)，雌激素联合RCC2 siRNA处理MCF-7细胞，检测细胞增殖与凋亡。结果 与乳腺纤维瘤组织相比，RCC2在雌激素受体阳性(ER+)乳腺癌组织中的表达水平升高(P=0.001)，在ER-乳腺癌组织中无升高(P=0.404)。抑制RCC2后ER+乳腺癌细胞系MCF-7增殖无变化(F处理=0.003，P=0.957)，但凋亡比例比Allstars siRNA组高(t=2.679，P=0.037)；雌二醇处理MCF-7后增殖能力增强(F=110.323，P

    【关键词】 染色体缩合调控子2；雌激素；乳腺癌；细胞凋亡；细胞增殖

    【Abstract】 Objective To explore the role of chromosomal condensation regulator 2 (RCC2) during the process of estrogen promoting the progression of breast cancer. Methods Human breast tissues were collected. Western blot was utilized to quantitatively detect the expression level of RCC2 protein. MCF-7 cells were treated with estrogen or RCC2 siRNA and combined use of estrogen and RCC2 siRNA. The cell apoptosis and cell proliferation were detected. Results Compared with the breast fibroma tissues ......