探讨建立大鼠半乳糖性白内障模型的方法(1)
【摘要】目的探讨建立大鼠半乳糖性白内障模型的方法。 方法48只大鼠，随机分为8组，每组6只，双眼均为观察眼，采用腹腔注射法建立大鼠半乳糖性白内障。每日对每只大鼠进行称量，A组：50%D半乳糖10 g/(kg·d)；B组：50%D半乳糖15 g/(kg·d)；C组：50% D半乳糖10 g/kg，每日2次；D组：50% D半乳糖15 g/kg，每日2次；E、F、G、H的注射剂量等同对应A、B、C、D组，A、B、C、D组在第10日开始加喝10% D半乳糖水。每3 d用裂隙灯观察晶状体的浑浊程度一次。结果观察至第30日时，除最低剂量的E组(3333%)外，各实验组的造模成功率均为100%，A～H各组的死亡率(%)分别为0、3333、6667、3333、0、1667、0、1667。总死亡率为2083%，造模成功率为7083%。结论腹腔注射半乳糖可以成功建立白内障动物模型，且造模的成功率较高，死亡率较低，是较稳定的半乳糖白内障动物模型。
【关键词】半乳糖；白内障；大鼠
Investigation of the method of establishment of rat models with galactose cataractZhong Lei， Wang Ting， Wang Tao， Li Weihua， Ling Shiqi Department of Ophthalmology， the Third Affiliated Hospital of Sun Yatsen University，Guangzhou 510630， China
Corresponding author，Ling Shiqi， Email：lingshiqi123@163com
【Abstract】ObjectiveTo explore the method of establishment of rat models with galactose cataract MethodsFortyeight rats were randomly divided into 8 groups(n= 6) Bilateral eyes were regarded as the observation eyes The rat models with galactose cataract were established by intraperitoneal injection Each rat was weighed daily In group A， 50% Dgalactose 10 g/kg was administered once daily In group B， 50% Dgalactose 15 g/kg was given once daily In group C， 50% Dgalactose 10 g/kg was administered twice daily In group D， 50% Dgalactose 15 g/kg was injected twice a day The injection doses in groups E， F， G and H corresponded to those in groups A， B， C and D， respectively In groups A， B， C and D， 10% Dgalactose water was supplemented on the 10th day The opacity of lenses was observed by the slitlamp biomicroscope every 3 d ResultsOn the 30th day， the success rate of each group was 100%， except for the group E (3333%) in which the lowest dose was administered The mortality rates in groups AH were 000%，3333%，6667%，3333%，000%，1667%，000%，1667%， respectively The total mortality rate was 2083%， and the success rate of model establishment was 7083% ConclusionIntraperitoneal injection of galactose can successfully establish the animal models of cataract with high success rate and low mortality rate The established rat model with galactose cataract is relatively stable
【Key words】Galactose； Cataract； Rat
糖尿病患者在2013年約达382亿，2035年预计达到592亿[1]。白内障是糖尿病的最常见并发症之一，更值得重视的是，白内障已被确定为全球视力障碍和失明的主要原因。对于临床研究与治疗，积极寻找病因是治疗及预防疾病最关键的步骤，因此建立稳定可靠的动物模型进行实验研究，有助于日后降低视力损害及白内障盲的发病率[2]。半乳糖诱导的白内障大鼠模型已广泛应用，是较好反映人类糖尿病性白内障和年龄相关性白内障的动物模型。半乳糖模型的建模方法一直存在争议，国外学者认为腹腔注射不能够形成糖性白内障[3]。但是在该领域，我国早期的一些学者发现腹腔注射半乳糖可诱发白内障的形成，还有学者采用球后注射、皮下注射等方法诱发白内障，造模的方式、方法与剂量都不相同，且引起的动物死亡率较高，基本达半数以上[4]。我们经过一系列的实验摸索，探讨半乳糖的用量及方法，旨在提供稳定可靠且死亡率较低的白内障动物模型制备方法，为日后白内障的实验研究及治疗提供一个参考方案。, 百拇医药(钟蕾 王婷 王涛 黎韦华 凌士奇)
【关键词】半乳糖；白内障；大鼠
Investigation of the method of establishment of rat models with galactose cataractZhong Lei， Wang Ting， Wang Tao， Li Weihua， Ling Shiqi Department of Ophthalmology， the Third Affiliated Hospital of Sun Yatsen University，Guangzhou 510630， China
Corresponding author，Ling Shiqi， Email：lingshiqi123@163com
【Abstract】ObjectiveTo explore the method of establishment of rat models with galactose cataract MethodsFortyeight rats were randomly divided into 8 groups(n= 6) Bilateral eyes were regarded as the observation eyes The rat models with galactose cataract were established by intraperitoneal injection Each rat was weighed daily In group A， 50% Dgalactose 10 g/kg was administered once daily In group B， 50% Dgalactose 15 g/kg was given once daily In group C， 50% Dgalactose 10 g/kg was administered twice daily In group D， 50% Dgalactose 15 g/kg was injected twice a day The injection doses in groups E， F， G and H corresponded to those in groups A， B， C and D， respectively In groups A， B， C and D， 10% Dgalactose water was supplemented on the 10th day The opacity of lenses was observed by the slitlamp biomicroscope every 3 d ResultsOn the 30th day， the success rate of each group was 100%， except for the group E (3333%) in which the lowest dose was administered The mortality rates in groups AH were 000%，3333%，6667%，3333%，000%，1667%，000%，1667%， respectively The total mortality rate was 2083%， and the success rate of model establishment was 7083% ConclusionIntraperitoneal injection of galactose can successfully establish the animal models of cataract with high success rate and low mortality rate The established rat model with galactose cataract is relatively stable
【Key words】Galactose； Cataract； Rat
糖尿病患者在2013年約达382亿，2035年预计达到592亿[1]。白内障是糖尿病的最常见并发症之一，更值得重视的是，白内障已被确定为全球视力障碍和失明的主要原因。对于临床研究与治疗，积极寻找病因是治疗及预防疾病最关键的步骤，因此建立稳定可靠的动物模型进行实验研究，有助于日后降低视力损害及白内障盲的发病率[2]。半乳糖诱导的白内障大鼠模型已广泛应用，是较好反映人类糖尿病性白内障和年龄相关性白内障的动物模型。半乳糖模型的建模方法一直存在争议，国外学者认为腹腔注射不能够形成糖性白内障[3]。但是在该领域，我国早期的一些学者发现腹腔注射半乳糖可诱发白内障的形成，还有学者采用球后注射、皮下注射等方法诱发白内障，造模的方式、方法与剂量都不相同，且引起的动物死亡率较高，基本达半数以上[4]。我们经过一系列的实验摸索，探讨半乳糖的用量及方法，旨在提供稳定可靠且死亡率较低的白内障动物模型制备方法，为日后白内障的实验研究及治疗提供一个参考方案。, 百拇医药(钟蕾 王婷 王涛 黎韦华 凌士奇)
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