mmiR936对人喉癌细胞株Hep2增殖、迁移、侵袭和药物敏感性的影响(1)
【摘要】目的探讨微小RNA936(miR936)对人类喉癌细胞株Hep2增殖、迁移、侵袭和药物敏感性的影响。方法通过慢病毒感染构建miR936过表达的喉癌细胞株Hep2细胞系(miR936组)及空载对照组(Vector组)。荧光显微镜观察病毒感染效率，实时荧光定量PCR检測2组细胞miR936的表达量。MTT增殖实验分析2组细胞增殖能力的变化。MTT药敏实验比较2组细胞药物敏感性的变化。划痕实验和Transwell小室实验分别检测2组细胞迁移和侵袭能力，激光共聚焦扫描显微镜观察细胞骨架变化。结果miR936组Hep2细胞的miR936mRNA表达水平高于Vector组(t=5600，P<005)。miR936组Hep2细胞增殖能力明显低于Vector组。培养24h和48h时miR936组Hep2细胞的迁移能力均低于Vector组(P均<001)；miR936组细胞的侵袭能力低于Vector组(P<001)；此外，miR936过表达后Hep2细胞的微管微丝与Vector组相比减少。药物敏感实验结果显示miR936组Hep2细胞对顺铂和阿霉素的敏感性较Vector组增加(均P<005)。结论miR936可以抑制Hep2细胞的增殖、迁移和侵袭，并且能够增加Hep2细胞对顺铂和阿霉素的敏感性。
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【关键词】喉肿瘤；微小RNA；增殖；迁移；侵袭；药物敏感性
EffectsofmicroRNA936ontheproliferation，migration，invasionanddrugsensitivityofhumanlaryngealcancercelllineHep2WangHaifeng，LiPei，WangZhiyuan，LinXijun，GuoCheng，YeJinDepartmentofOtolaryngologyHeadNeckSurgery，theThirdAffiliatedHospitalofSunYatsenUniversity，Guangzhou510630，China
Correspondingauthor，YeJin，Email：yejin_sums@aliyuncom
【Abstract】ObjectiveToinvestigatetheeffectofmicroRNA936(miR936)ontheproliferation，migration，invasionanddrugsensitivityofhumanlaryngealcancercelllineHep2MethodsTheHep2celllineoverexpressingmiR936(miR936group)andtheemptyvectorcontrolgroup(vectorgroup)wereconstructedbylentivirusinfectionTheefficiencyofvirusinfectionwasobservedbyfluorescencemicroscopeTheexpressionofmiR936wasdetectedbyRTqPCRMTTproliferationassaywasusedtoanalyzethechangesofcellproliferationinbothgroupsMTTdrugsensitivityassaywasperformedtocomparethechangesofdrugsensitivityinbothgroupsCellmigrationandinvasionabilityintwogroupswasevaluatedbyTranswellchamberassayConfocalmicroscopewasadoptedtoobservethevariationsincellcytoskeletonResultsTheexpressionlevelofmiR936mRNAofHep2cellsinthemiR936groupwassignificantlyhigherthanthatinthevectorgroup(t=5600，P<005)InthemiR936group，theproliferationcapacityofHep2cellswassignificantlylowercomparedwiththatinthevectorgroupThemigrationabilityofHep2cellsinthemiR936groupwassignificantlylowerthanthatinthevectorgroupat24hand48hafterculture(bothP<001)ThecellinvasionabilityinthemiR936groupwasconsiderablylowerthanthatinthevectorgroup(P<001)ThequantityofmicrotubulesofHep2cellswassignificantlyreducedafteroverexpressionofmiR936comparedwiththatinthevectorgroupDrugsensitivitytestdemonstratedthatthesensitivityofHep2cellstocisplatinandadriamycinwassignificantlyincreasedinthemiR936groupcomparedwiththatinthevectorgroup(P<001)ConclusionmiR936caninhibittheproliferation，migrationandinvasionofHep2cells，andcanincreasethesensitivityofHep2cellstocisplatinandadriamycin, 百拇医药(王海峰 李培 王志远 林惜君 郭程 叶进)
, http://www.100md.com
【关键词】喉肿瘤；微小RNA；增殖；迁移；侵袭；药物敏感性
EffectsofmicroRNA936ontheproliferation，migration，invasionanddrugsensitivityofhumanlaryngealcancercelllineHep2WangHaifeng，LiPei，WangZhiyuan，LinXijun，GuoCheng，YeJinDepartmentofOtolaryngologyHeadNeckSurgery，theThirdAffiliatedHospitalofSunYatsenUniversity，Guangzhou510630，China
Correspondingauthor，YeJin，Email：yejin_sums@aliyuncom
【Abstract】ObjectiveToinvestigatetheeffectofmicroRNA936(miR936)ontheproliferation，migration，invasionanddrugsensitivityofhumanlaryngealcancercelllineHep2MethodsTheHep2celllineoverexpressingmiR936(miR936group)andtheemptyvectorcontrolgroup(vectorgroup)wereconstructedbylentivirusinfectionTheefficiencyofvirusinfectionwasobservedbyfluorescencemicroscopeTheexpressionofmiR936wasdetectedbyRTqPCRMTTproliferationassaywasusedtoanalyzethechangesofcellproliferationinbothgroupsMTTdrugsensitivityassaywasperformedtocomparethechangesofdrugsensitivityinbothgroupsCellmigrationandinvasionabilityintwogroupswasevaluatedbyTranswellchamberassayConfocalmicroscopewasadoptedtoobservethevariationsincellcytoskeletonResultsTheexpressionlevelofmiR936mRNAofHep2cellsinthemiR936groupwassignificantlyhigherthanthatinthevectorgroup(t=5600，P<005)InthemiR936group，theproliferationcapacityofHep2cellswassignificantlylowercomparedwiththatinthevectorgroupThemigrationabilityofHep2cellsinthemiR936groupwassignificantlylowerthanthatinthevectorgroupat24hand48hafterculture(bothP<001)ThecellinvasionabilityinthemiR936groupwasconsiderablylowerthanthatinthevectorgroup(P<001)ThequantityofmicrotubulesofHep2cellswassignificantlyreducedafteroverexpressionofmiR936comparedwiththatinthevectorgroupDrugsensitivitytestdemonstratedthatthesensitivityofHep2cellstocisplatinandadriamycinwassignificantlyincreasedinthemiR936groupcomparedwiththatinthevectorgroup(P<001)ConclusionmiR936caninhibittheproliferation，migrationandinvasionofHep2cells，andcanincreasethesensitivityofHep2cellstocisplatinandadriamycin, 百拇医药(王海峰 李培 王志远 林惜君 郭程 叶进)
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