HBV前C区及基因启动子区基因变异与乙肝肝硬化相关性初步研究(1)
【摘要】 目的 探讨HBV前C(PC)区和基本核心启动子(BCP)区与乙型肝炎患者病情进展的关系。方法 选择37例慢性乙型肝炎患者(慢乙肝组)及27例乙型肝炎肝硬化患者(肝硬化组)，分析2组患者的一般资料和临床特征，对2组患者血清样本进行A1762T/G1764A、G1896A基因突变检测，比较组间差异。结果 肝硬化组患者的年龄大于慢乙肝组，ALT、HBV DNA水平低于慢乙肝组，层粘连蛋白水平高于慢乙肝组(P均< 0.05)。肝硬化组患者A1762T/G1764A双位点突变发生率为78%，高于慢乙肝组的49%(P < 0.05);肝硬化组G1896A突变发生率为48%，慢乙肝组为60%，2组比较差异无统计学意义(P > 0.05)。结论 乙肝肝硬化患者HBV PC/BCP区A1762T/G1764A突变率高于慢乙肝患者，A1762T/G1764A突变可能参与慢乙肝发展至肝硬化的过程。
【关键词】 慢性乙型肝炎;乙肝肝硬化;前C区突变;基本核心启动子区突变
Preliminary analysis of correlation between gene mutation in pre-core and basic core promoter of hepatitis B virus and hepatitis B-induced liver cirrhosis Wei Fangfang， Li Wenli， Zhen Limin， Liao Changbin， Xu Ruirong， Wu Maosheng. Department of Infectious Disease， Guangdong Second Provincial General Hospital， Guangzhou 510317， China
Corresponding author， Wu Maosheng， E-mail： wu.maosheng@ 163. com
【Abstract】 Objective To explore the correlation between the gene mutations in hepatitis B virus (HBV) pre-core (PC) and basic core promoter (BCP) and the progression of hepatitis B. Methods A total of 37 patients with chronic hepatitis B (CHB) and 27 patients with CHB-induced liver cirrhosis were recruited into the CHB and liver cirrhosis groups. Baseline data and clinical characteristics were analyzed in two groups. The gene mutations of A1762T/G1764A and G1896A in the serum samples were detected and compared between two groups. Results The age of liver cirrhosis patients was significantly older， the ALT and HBV DNA levels were considerably lower and the laminin level was significantly higher compared with those in the CHB group (all P < 0.05). The incidence rate of of A1762T/G1764A double mutations in the liver cirrhosis group was 78%， significantly higher than 49% in CHB patients (P < 0.05). The incidence rate of G1896A mutation did not significantly differ between the CHB and liver cirrhosis groups (60% vs. 48%， P > 0.05). Conclusions The prevalence of A1762T/G1764A double mutations in the PC/BCP of patients with liver cirrhosis is significantly higher than that in CHB patients. A1762T/G1764A mutation probably participates in the progression from CHB into liver cirrhosis.
【Key words】 Chronic hepatitis B;Hepatitis B-induced liver cirrhosis;Pre-core mutation;
Basic core promoter mutation
HBV前 C (PC)區和基本核心启动子(BCP)区基因变异后病毒复制能力增强、机体免疫原性改变和致病性增强，影响机体的免疫应答情况，使HBV逃避宿主的免疫清除，导致持续感染并加重肝细胞损害[1]。PC/BCP区基因变异是导致HBV感染病情加重的重要原因。PC/BCP区最常见突变位点是G1896A、A1762T/G1764A，既往研究表明G1896A、A1762T/G1764A突变会加重乙型肝炎患者病情，导致重型肝炎、肝癌的发生[2]。然而，有关PC/BCP区突变与肝硬化相关性研究却很少报道，本研究通过对慢性乙型肝炎(慢乙肝)与乙型肝炎肝硬化(乙肝肝硬化)患者G1896A、A1762T/G1764A发生率进行比较，进一步探索PC/BCP区突变在乙型肝炎病情进展中作用。, http://www.100md.com(魏芳芳?李文莉?甄丽敏?廖常斌?许瑞荣?吴茂盛)
【关键词】 慢性乙型肝炎;乙肝肝硬化;前C区突变;基本核心启动子区突变
Preliminary analysis of correlation between gene mutation in pre-core and basic core promoter of hepatitis B virus and hepatitis B-induced liver cirrhosis Wei Fangfang， Li Wenli， Zhen Limin， Liao Changbin， Xu Ruirong， Wu Maosheng. Department of Infectious Disease， Guangdong Second Provincial General Hospital， Guangzhou 510317， China
Corresponding author， Wu Maosheng， E-mail： wu.maosheng@ 163. com
【Abstract】 Objective To explore the correlation between the gene mutations in hepatitis B virus (HBV) pre-core (PC) and basic core promoter (BCP) and the progression of hepatitis B. Methods A total of 37 patients with chronic hepatitis B (CHB) and 27 patients with CHB-induced liver cirrhosis were recruited into the CHB and liver cirrhosis groups. Baseline data and clinical characteristics were analyzed in two groups. The gene mutations of A1762T/G1764A and G1896A in the serum samples were detected and compared between two groups. Results The age of liver cirrhosis patients was significantly older， the ALT and HBV DNA levels were considerably lower and the laminin level was significantly higher compared with those in the CHB group (all P < 0.05). The incidence rate of of A1762T/G1764A double mutations in the liver cirrhosis group was 78%， significantly higher than 49% in CHB patients (P < 0.05). The incidence rate of G1896A mutation did not significantly differ between the CHB and liver cirrhosis groups (60% vs. 48%， P > 0.05). Conclusions The prevalence of A1762T/G1764A double mutations in the PC/BCP of patients with liver cirrhosis is significantly higher than that in CHB patients. A1762T/G1764A mutation probably participates in the progression from CHB into liver cirrhosis.
【Key words】 Chronic hepatitis B;Hepatitis B-induced liver cirrhosis;Pre-core mutation;
Basic core promoter mutation
HBV前 C (PC)區和基本核心启动子(BCP)区基因变异后病毒复制能力增强、机体免疫原性改变和致病性增强，影响机体的免疫应答情况，使HBV逃避宿主的免疫清除，导致持续感染并加重肝细胞损害[1]。PC/BCP区基因变异是导致HBV感染病情加重的重要原因。PC/BCP区最常见突变位点是G1896A、A1762T/G1764A，既往研究表明G1896A、A1762T/G1764A突变会加重乙型肝炎患者病情，导致重型肝炎、肝癌的发生[2]。然而，有关PC/BCP区突变与肝硬化相关性研究却很少报道，本研究通过对慢性乙型肝炎(慢乙肝)与乙型肝炎肝硬化(乙肝肝硬化)患者G1896A、A1762T/G1764A发生率进行比较，进一步探索PC/BCP区突变在乙型肝炎病情进展中作用。, http://www.100md.com(魏芳芳?李文莉?甄丽敏?廖常斌?许瑞荣?吴茂盛)
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