高糖状态通过WIF1-Wnt/β-catenin通路调控结肠肿瘤细胞的增殖
林志玲?黎洁瑶?闵筱辉?许稷豪?于涛?陈其奎【摘要】目的 分析高糖狀态对结肠肿瘤细胞增殖的影响,并探讨Wnt抑制因子1(WIF1)通过Wnt/β-连环蛋白(β-catenin)通路影响高糖状态下结肠肿瘤细胞增殖的机制。方法 用不同浓度的D-葡萄糖处理结肠肿瘤细胞株SW620细胞,通过实时荧光定量PCR(qPCR)和蛋白免疫印迹法测定增殖相关基因的表达,行细胞增殖活性检测、细胞计数实验,用细胞平板克隆形成实验检测细胞增殖情况,采用qPCR和蛋白免疫印迹法测定WIF1和β-catenin的表达。转染siRNA和过表达质粒调控WIF1的表达后,检测WIF1对SW620细胞增殖能力和β-catenin表达水平的影响。结果 随着糖浓度的增加,SW620细胞的增殖能力增强(P均< 0.05),WIF1的表达下降而β-catenin的表达增高(P均< 0.05)。下调WIF1的表达,SW620细胞的增殖能力增强且β-catenin的表达增高(P均< 0.05),而过表达WIF1后,SW620细胞的增殖能力受到抑制且β-catenin的表达下降(P均< 0.05)。结论 高糖状态下WIF1表达的下降,可能通过活化Wnt/β-catenin通路促进高糖状态下结肠肿瘤细胞的增殖。
【关键词】高糖;结肠肿瘤;Wnt抑制因子1;β-连环蛋白;增殖
High glucose regulates the proliferation of colon cancer cells through the WIF1-Wnt/β-catenin signaling pathway Lin Zhiling, Li Jieyao, Min Xiaohui, Xu Jihao, Yu Tao, Chen Qikui. Department of Gastroenterology, Sun Yat-sen Memorial Hospital of Sun Yat-sen University, Guangzhou 510120, China
Corresponding author, Chen Qikui, E-mail: qkchen2015@ 163. com
【Abstract】Objective To evaluate the effect of high glucose on the proliferation of colon cancer cells, and investigate the mechanism of the effect of Wnt inhibitory factor 1 (WIF1) on the proliferation of colon cancer cells under high glucose state through the Wnt/β-catenin signaling pathway. Methods Colon cancer cell line SW620 cells were treated with different doses of D-glucose. The expressions of proliferation-associated mRNA and protein were measured by real-time fluorescent quantitative PCR (qPCR) and Western blot. The proliferation ability of SW620 cells was measured by cell proliferation assay ......
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