苗药赤胫散提取物的体外抑菌活性研究(1)
摘 要 目的：研究苗藥赤胫散提取物的体外抑菌活性。方法：采用管碟法，以氯霉素和氟康唑为阳性对照，测定赤胫散的水提物和95%乙醇提取物及其乙酸乙酯、正丁醇部位萃取物对金黄色葡萄球菌、大肠埃希菌、福氏志贺氏菌、伤寒杆菌、枯草芽孢杆菌、绿脓杆菌、乙型溶血性链球菌、白色念珠菌、新生隐球菌等9种菌株的抑菌作用，筛选出具有抑菌作用的部位及对药物敏感的试验菌株。分别采用微量肉汤稀释法和琼脂培养基平板法，测定赤胫散95%乙醇提取物及其不同萃取部位对敏感菌株的最低抑菌浓度(MIC)和最低杀菌浓度(MBC)。结果：赤胫散的水提物几乎无抑菌活性；95%乙醇提取物对各菌株表现出不同程度的抑制作用，对伤寒杆菌等6种细菌的抑菌作用优于乙型溶血性链球菌及2种真菌；95%乙醇提取物的乙酸乙酯及正丁醇萃取部位表现出良好的抑菌作用，且乙酸乙醇萃取部位强于正丁醇部位，但对真菌均未见抑制作用；95%乙醇提取物对上述6种细菌的MIC、MBC分别为6.25～12.5、12.5～25 mg/mL，其乙酸乙酯部位的 MIC、MBC 分别为3.13～6.25、6.25～12.5 mg/mL，其正丁醇部位的 MIC、MBC 分别为6.25～12.5、12.5～25 mg/mL。结论：苗药赤胫散的95%乙醇提取物及其乙酸乙酯、正丁醇部位萃取物对伤寒杆菌等6种常见细菌具有明显的体外抑菌作用。
关键词 赤胫散；乙醇提取物；萃取部位；体外抑菌作用；最低抑菌浓度；最低杀菌浓度
Study on in vitro Bacteriostatic Activity of the Extracts from Miao Medicine Polygonum runcinatum
LIU Lan，HUANG Jiayu，CHEN Jun，MU Yanhua，ZHOU Xianxia，PAN Liucen，GUO Yurou，LI Li(School of Pharmacy， Guizhou Medical University， Guiyang 550025， China)
ABSTRACT OBJECTIVE： To study in vitro bacteriostatic activity of the extracts from Miao medicine Polygonum runcinatum. METHODS： Using chloramphenicol and fluconazole as positive control， the inhibitory effects of water extract， 95% ethanol extract and its ethyl acetate and n-butanol fraction on 9 kinds of strains were determined by cup plate method， such as Staphylococcus aureus， Escherichia coli， Shigella flexneri， Salmonella typhi， Bacillus subtilis， Pseudomonas aeruginosa， type B Hemolytic streptococcus， Candida albicans and Cryptococcus neoformans. The parts with bacteriostatic activity and trial strains sensitive to drug were screened. Micro-broth dilution method and agar culture medium plate method were used to determine MIC and MBC of 95% ethanol extract and its fractions of P. runcinatum to sensitive strains. RESULTS： The water extract of P. runcinatum almost had no inhibitory effect. 95% ethanol extract showed different degrees of bacteriostatic activity to strains； bacteriostatic effects of it to 6 kinds of bacteria as S. typhi was better than that of type B H. streptococcus and 2 kinds of fungus. The ethyl acetate and n-butanol fraction of 95% ethanol extracts showed good bacteriostatic effect and the ethyl acetate fraction had stronger effect， while all the fractions of 95% ethanol extracts showed no inhibitory effect on fungus. MIC and MBC of 95% ethanol extract to above 6 kinds of bacteria were 6.25-12.5 and 12.5-25 mg/mL， respectively； MIC and MBC of ethyl acetate fraction were 3.13-6.25， 6.25-12.5 mg/mL； MIC and MBC of n-butanol fraction were 6.25-12.5， 12.5-25 mg/mL. CONCLUSIONS： 95% ethanol extract， ethyl acetate and n-butanol fractions of Miao medicine P. runcinatum have obvious in vitro bacteriostatic activity to 6 common bacterias as S. typhi., http://www.100md.com(刘兰 黄家宇 陈俊 母艳华 周贤霞 潘柳岑 郭雨柔 李莉)
关键词 赤胫散；乙醇提取物；萃取部位；体外抑菌作用；最低抑菌浓度；最低杀菌浓度
Study on in vitro Bacteriostatic Activity of the Extracts from Miao Medicine Polygonum runcinatum
LIU Lan，HUANG Jiayu，CHEN Jun，MU Yanhua，ZHOU Xianxia，PAN Liucen，GUO Yurou，LI Li(School of Pharmacy， Guizhou Medical University， Guiyang 550025， China)
ABSTRACT OBJECTIVE： To study in vitro bacteriostatic activity of the extracts from Miao medicine Polygonum runcinatum. METHODS： Using chloramphenicol and fluconazole as positive control， the inhibitory effects of water extract， 95% ethanol extract and its ethyl acetate and n-butanol fraction on 9 kinds of strains were determined by cup plate method， such as Staphylococcus aureus， Escherichia coli， Shigella flexneri， Salmonella typhi， Bacillus subtilis， Pseudomonas aeruginosa， type B Hemolytic streptococcus， Candida albicans and Cryptococcus neoformans. The parts with bacteriostatic activity and trial strains sensitive to drug were screened. Micro-broth dilution method and agar culture medium plate method were used to determine MIC and MBC of 95% ethanol extract and its fractions of P. runcinatum to sensitive strains. RESULTS： The water extract of P. runcinatum almost had no inhibitory effect. 95% ethanol extract showed different degrees of bacteriostatic activity to strains； bacteriostatic effects of it to 6 kinds of bacteria as S. typhi was better than that of type B H. streptococcus and 2 kinds of fungus. The ethyl acetate and n-butanol fraction of 95% ethanol extracts showed good bacteriostatic effect and the ethyl acetate fraction had stronger effect， while all the fractions of 95% ethanol extracts showed no inhibitory effect on fungus. MIC and MBC of 95% ethanol extract to above 6 kinds of bacteria were 6.25-12.5 and 12.5-25 mg/mL， respectively； MIC and MBC of ethyl acetate fraction were 3.13-6.25， 6.25-12.5 mg/mL； MIC and MBC of n-butanol fraction were 6.25-12.5， 12.5-25 mg/mL. CONCLUSIONS： 95% ethanol extract， ethyl acetate and n-butanol fractions of Miao medicine P. runcinatum have obvious in vitro bacteriostatic activity to 6 common bacterias as S. typhi., http://www.100md.com(刘兰 黄家宇 陈俊 母艳华 周贤霞 潘柳岑 郭雨柔 李莉)