蒙药圆柏的质量标准研究(1)
中圖分类号 R926 文献标志码 A 文章编号 1001-0408(2019)01-0088-06
DOI 10.6039/j.issn.1001-0408.2019.01.19
摘 要 目的：建立蒙药圆柏的质量标准，为其质量控制提供参考。方法：收集内蒙古地区的圆柏药材，共9批。观察圆柏样品的性状并对其枝叶横切面和粉末进行显微鉴别；采用薄层色谱(TLC)法对圆柏样品进行定性分析；按2015年版《中国药典》(四部)通则方法测定圆柏样品中水分、总灰分、酸不溶性灰分含量和醇溶性浸出物含量；采用高效液相色谱法测定圆柏样品中槲皮苷和穗花杉双黄酮含量。结果：圆柏小枝呈圆柱形或四菱形，刺叶长短不等，呈深绿色或黄绿色；显微观察发现，鳞叶表皮细胞排列整齐、鳞叶中部有较大空腔、海绵组织细胞较大，表皮细胞类长方形，气孔较多，保卫细胞较大；TLC结果显示，在对照品(槲皮苷和芦丁)色谱相应位置上显相同颜色的荧光斑点；9批样品中水分含量为3.2%～5.6%，总灰分含量为3.4%～5.8%，酸不溶性灰分含量均不超过0.8%，醇溶性浸出物含量为28.0%～33.8%，槲皮苷含量为0.11%～0.28%，穗花杉双黄酮含量为0.15%～0.25%。结论：初步拟定圆柏中水分含量不得超过8.0%、总灰分含量不得超过7.0%、醇溶性浸出物含量不得低于24.0%、槲皮苷含量不得低于0.11%、穗花杉双黄酮含量不得低于0.15%，制订的标准可用于圆柏药材的质量控制。
关键词 蒙药；圆柏；质量标准；薄层色谱鉴别；高效液相色谱法；含量测定
Study on Quality Standard of Mongolia Medicine Sabina vulgaris
LI Zhen1，YANG Yang1，MENG Meiying1，XIAO Bin2(1.Ordos Testing&Research Center of Drug and Medical Devices， Inner Mongolia Ordos 017000， China；2.Clinical Pharmacy Laboratory， Ordos Central Hospital， Inner Mongolia Ordos 017000， China)
ABSTRACT OBJECTIVE： To establish the quality standard of Sabina vulgaris， and to provide reference for its quality control. METHODS： Totally 9 batches of S. vulgaris were collected from Inner Mongolia area. The characteristics of S. vulgaris samples were observed and microscopic identification was conducted for the transverse section and powder of its branches and leaves. TLC was used for qualitative analysis of S. vulgaris. The contents of water， total ash， acid-insoluble ash and alcohol-soluble extract in S. vulgaris were determined according to the method stated in 2015 edition of Chinese Pharmacopeia (part Ⅳ). The contents of quercetin and amentoflavone in S. vulgaris were determined by HPLC. RESULTS： The branchlets of S. vulgaris were cylindrical or rhombic， with varying length of thorns， dark green or yellowish green. The microscopic observation showed that the epidermal cells of scale leaves were arranged orderly； there were larger cavities in the middle of scale leaves， large cells in spongy tissue； epidermal cells were rectangular with more stomata and large guard cells. Results of TLC showed that the spots of the same color were found in the corresponding positions of chromatogram for substance control (dendrooside and rutin). In the 9 batch of samples， the contents of water were 3.2%-5.6%； the contents of total ash were 3.4%-5.8%； the contents of acid-insoluble ash were lower than 0.8%； the contents of alcohol-soluble extract were 28.0%-33.8%； the contents of quercetin were 0.11%-0.28%； the contents of amentoflavone were 0.15%-0.25%. CONCLUSIONS： It is preliminarily proposed that the content of water in S. vulgaris is not more than 8.0%； the content of total ash is not more than 7.0%； the content of alcohol-soluble extract is not less than 24.0%； the content of quercetin is not less than 0.11%； the content of amentoflavone is not less than 0.15%. Established standard can be used for the quality control of S. vulgaris., 百拇医药(李珍 杨洋 孟美英 肖斌)
DOI 10.6039/j.issn.1001-0408.2019.01.19
摘 要 目的：建立蒙药圆柏的质量标准，为其质量控制提供参考。方法：收集内蒙古地区的圆柏药材，共9批。观察圆柏样品的性状并对其枝叶横切面和粉末进行显微鉴别；采用薄层色谱(TLC)法对圆柏样品进行定性分析；按2015年版《中国药典》(四部)通则方法测定圆柏样品中水分、总灰分、酸不溶性灰分含量和醇溶性浸出物含量；采用高效液相色谱法测定圆柏样品中槲皮苷和穗花杉双黄酮含量。结果：圆柏小枝呈圆柱形或四菱形，刺叶长短不等，呈深绿色或黄绿色；显微观察发现，鳞叶表皮细胞排列整齐、鳞叶中部有较大空腔、海绵组织细胞较大，表皮细胞类长方形，气孔较多，保卫细胞较大；TLC结果显示，在对照品(槲皮苷和芦丁)色谱相应位置上显相同颜色的荧光斑点；9批样品中水分含量为3.2%～5.6%，总灰分含量为3.4%～5.8%，酸不溶性灰分含量均不超过0.8%，醇溶性浸出物含量为28.0%～33.8%，槲皮苷含量为0.11%～0.28%，穗花杉双黄酮含量为0.15%～0.25%。结论：初步拟定圆柏中水分含量不得超过8.0%、总灰分含量不得超过7.0%、醇溶性浸出物含量不得低于24.0%、槲皮苷含量不得低于0.11%、穗花杉双黄酮含量不得低于0.15%，制订的标准可用于圆柏药材的质量控制。
关键词 蒙药；圆柏；质量标准；薄层色谱鉴别；高效液相色谱法；含量测定
Study on Quality Standard of Mongolia Medicine Sabina vulgaris
LI Zhen1，YANG Yang1，MENG Meiying1，XIAO Bin2(1.Ordos Testing&Research Center of Drug and Medical Devices， Inner Mongolia Ordos 017000， China；2.Clinical Pharmacy Laboratory， Ordos Central Hospital， Inner Mongolia Ordos 017000， China)
ABSTRACT OBJECTIVE： To establish the quality standard of Sabina vulgaris， and to provide reference for its quality control. METHODS： Totally 9 batches of S. vulgaris were collected from Inner Mongolia area. The characteristics of S. vulgaris samples were observed and microscopic identification was conducted for the transverse section and powder of its branches and leaves. TLC was used for qualitative analysis of S. vulgaris. The contents of water， total ash， acid-insoluble ash and alcohol-soluble extract in S. vulgaris were determined according to the method stated in 2015 edition of Chinese Pharmacopeia (part Ⅳ). The contents of quercetin and amentoflavone in S. vulgaris were determined by HPLC. RESULTS： The branchlets of S. vulgaris were cylindrical or rhombic， with varying length of thorns， dark green or yellowish green. The microscopic observation showed that the epidermal cells of scale leaves were arranged orderly； there were larger cavities in the middle of scale leaves， large cells in spongy tissue； epidermal cells were rectangular with more stomata and large guard cells. Results of TLC showed that the spots of the same color were found in the corresponding positions of chromatogram for substance control (dendrooside and rutin). In the 9 batch of samples， the contents of water were 3.2%-5.6%； the contents of total ash were 3.4%-5.8%； the contents of acid-insoluble ash were lower than 0.8%； the contents of alcohol-soluble extract were 28.0%-33.8%； the contents of quercetin were 0.11%-0.28%； the contents of amentoflavone were 0.15%-0.25%. CONCLUSIONS： It is preliminarily proposed that the content of water in S. vulgaris is not more than 8.0%； the content of total ash is not more than 7.0%； the content of alcohol-soluble extract is not less than 24.0%； the content of quercetin is not less than 0.11%； the content of amentoflavone is not less than 0.15%. Established standard can be used for the quality control of S. vulgaris., 百拇医药(李珍 杨洋 孟美英 肖斌)