茉莉酸甲酯对滇龙胆叶中4种裂环烯醚萜类物质含量的影响(1)
中图分类号 R282.2 文献标志码 A 文章编号 1001-0408(2019)03-0344-05
DOI 10.6039/j.issn.1001-0408.2019.03.13
摘 要 目的：研究外源性茉莉酸甲酯对滇龙胆叶中4种裂环烯醚萜类物质(龙胆苦苷、獐牙菜苦苷、獐牙菜苷和苦龙胆酯苷)含量及生理生化指标的影响，为茉莉酸甲酯在滇龙胆规范化种植中的应用提供科学依据。方法：分别以10、50、100、200 mg/L茉莉酸甲酯喷施滇龙胆植株2、4、6、8、10 d(喷施量均为500 mL)后，收集药材叶为样品材料，并收集相应时段未喷施处理的药材叶为对照。采用高效液相色谱(HPLC)法测定4个质量浓度下茉莉酸甲酯喷施10 d后滇龙胆叶中4种裂环烯醚萜类物质含量，优选茉莉酸甲酯的最佳喷施质量浓度(有效成分含量最高)。采用HPLC法测定以最佳喷施质量浓度茉莉酸甲酯喷施不同时间后滇龙胆叶4种裂环烯醚萜類物质的含量，并测定其中相关生理生化指标(超氧化物歧化酶、过氧化物酶、过氧化氢酶和丙二醛)水平。结果：以10、50、100、200 mg/L茉莉酸甲酯喷施滇龙胆植株10 d后，叶中4种裂环烯醚萜类物质的含量较未处理叶均不同程度增加，其中以100 mg/L茉莉酸甲酯喷施后效果最好，此时叶中龙胆苦苷、獐牙菜苦苷和獐牙菜苷的含量分别为未处理叶的1.88、2.36和1.87倍(P<0.05)。以100 mg/L茉莉酸甲酯喷施2、4、6、8、10 d后，叶中4种裂环烯醚萜类物质的含量均不同程度高于同时段未处理叶；在喷施4 d后叶中龙胆苦苷含量开始出现显著差异(P<0.05)，在喷施6 d后叶中4种裂环烯醚萜类物质的含量总体较高，此时叶中龙胆苦苷、獐牙菜苦苷、獐牙菜苷和苦龙胆酯苷含量分别为同时段未处理叶的1.88、1.88、1.47、1.82倍(P<0.05)；并自喷施4 d起，叶中各生理生化指标水平较未处理叶均显著升高(P<0.05)。结论：以100 mg/L茉莉酸甲酯喷施6 d后滇龙胆叶中4种裂环烯醚萜类物质含量升高最明显，这可能与其提高了叶中相关生理生化指标的水平有关。
关键词 滇龙胆叶；高效液相色谱法；茉莉酸甲酯；裂环烯醚萜类物质；含量测定；生理生化指标
ABSTRACT OBJECTIVE： To study the effects of exogenous methyl jasmonate on the contents of 4 kinds of secoiridoid substance (gentiopicrin， swertiamain， sweroside and amarogentin) and biochemical indexes in the leaves of Gentiana rigescens， and to provide scientific evidence for the application of methyl jasmonate in standardized planting of G. rigescens. METHODS： 2， 4， 6， 8， 10 d after spraying 10， 50， 100， 200 mg/L methyl jasmonate (spraying amount was 500 mL) for G. rigescens strain， the leaves of medicinal material were collected as sample， and the other leaves without spraying methyl jasmonate were collected as control. HPLC method was used to determine the contents of 4 kinds of secoiridoid substance in the leaves of G. rigescens after spraying 4 kinds of concentrations of methyl jasmonate for 10 d. The concentration of methyl jasmonate was optimized (the content of effective component was the highest). HPLC method was adopted to determine the contents of 4 kinds of secoiridoid substance in the leaves of G. rigescens after spraying the optimal concentration of methyl jasmonate for different time. The levels of relevant biochemical indexes (SOD， POD， CAT， MDA) were determined. RESULTS： 10 d after spraying 10， 50， 100， 200 mg/L methyl jasmonate for G. rigescens strains， the contents of 4 kinds of secoiridoid substance in the leaves were increased to different extent. Compared with untreated leaves， 100 mg/L methyl jasmonate had the best effect after spraying， and the contents of gentiopicrin， swertiamain and sweroside in treated leaves were 1.88， 2.36 and 1.87 times of those in untreated leaves， respectively (P<0.05). 2， 4， 6， 8， 10 d after spraying 100 mg/L methyl jasmonate， the contents of 4 kinds of secoiridoid substance in treated leaves were higher than untreated leaves at corresponding stage； the content of secoiridoid had significant difference after spraying for 4 d (P<0.05). The contents of active components in general were relatively high after spraying for 6 d， and the contents of gentiopicrin， swertiamain， sweroside and amarogentin in treated leaves were 1.88， 1.88， 1.47， 1.82 times of those in untreated leaves， respectively (P<0.05). The levels of relevant biochemical indexes in treated leaves were increased significantly since 4 d of spraying， compared with untreated leaves (P<0.05). CONCLUSIONS： After spraying 100 mg/L methyl jasmonate for 6 d， the contents increase of 4 kinds of secoiridoid substance in the leaves of G. rigescens are most obvious， which may be associated with improving the levels of related biochemical indexes., 百拇医药(金琳 李海峰 刘卫红 胡侃)
DOI 10.6039/j.issn.1001-0408.2019.03.13
摘 要 目的：研究外源性茉莉酸甲酯对滇龙胆叶中4种裂环烯醚萜类物质(龙胆苦苷、獐牙菜苦苷、獐牙菜苷和苦龙胆酯苷)含量及生理生化指标的影响，为茉莉酸甲酯在滇龙胆规范化种植中的应用提供科学依据。方法：分别以10、50、100、200 mg/L茉莉酸甲酯喷施滇龙胆植株2、4、6、8、10 d(喷施量均为500 mL)后，收集药材叶为样品材料，并收集相应时段未喷施处理的药材叶为对照。采用高效液相色谱(HPLC)法测定4个质量浓度下茉莉酸甲酯喷施10 d后滇龙胆叶中4种裂环烯醚萜类物质含量，优选茉莉酸甲酯的最佳喷施质量浓度(有效成分含量最高)。采用HPLC法测定以最佳喷施质量浓度茉莉酸甲酯喷施不同时间后滇龙胆叶4种裂环烯醚萜類物质的含量，并测定其中相关生理生化指标(超氧化物歧化酶、过氧化物酶、过氧化氢酶和丙二醛)水平。结果：以10、50、100、200 mg/L茉莉酸甲酯喷施滇龙胆植株10 d后，叶中4种裂环烯醚萜类物质的含量较未处理叶均不同程度增加，其中以100 mg/L茉莉酸甲酯喷施后效果最好，此时叶中龙胆苦苷、獐牙菜苦苷和獐牙菜苷的含量分别为未处理叶的1.88、2.36和1.87倍(P<0.05)。以100 mg/L茉莉酸甲酯喷施2、4、6、8、10 d后，叶中4种裂环烯醚萜类物质的含量均不同程度高于同时段未处理叶；在喷施4 d后叶中龙胆苦苷含量开始出现显著差异(P<0.05)，在喷施6 d后叶中4种裂环烯醚萜类物质的含量总体较高，此时叶中龙胆苦苷、獐牙菜苦苷、獐牙菜苷和苦龙胆酯苷含量分别为同时段未处理叶的1.88、1.88、1.47、1.82倍(P<0.05)；并自喷施4 d起，叶中各生理生化指标水平较未处理叶均显著升高(P<0.05)。结论：以100 mg/L茉莉酸甲酯喷施6 d后滇龙胆叶中4种裂环烯醚萜类物质含量升高最明显，这可能与其提高了叶中相关生理生化指标的水平有关。
关键词 滇龙胆叶；高效液相色谱法；茉莉酸甲酯；裂环烯醚萜类物质；含量测定；生理生化指标
ABSTRACT OBJECTIVE： To study the effects of exogenous methyl jasmonate on the contents of 4 kinds of secoiridoid substance (gentiopicrin， swertiamain， sweroside and amarogentin) and biochemical indexes in the leaves of Gentiana rigescens， and to provide scientific evidence for the application of methyl jasmonate in standardized planting of G. rigescens. METHODS： 2， 4， 6， 8， 10 d after spraying 10， 50， 100， 200 mg/L methyl jasmonate (spraying amount was 500 mL) for G. rigescens strain， the leaves of medicinal material were collected as sample， and the other leaves without spraying methyl jasmonate were collected as control. HPLC method was used to determine the contents of 4 kinds of secoiridoid substance in the leaves of G. rigescens after spraying 4 kinds of concentrations of methyl jasmonate for 10 d. The concentration of methyl jasmonate was optimized (the content of effective component was the highest). HPLC method was adopted to determine the contents of 4 kinds of secoiridoid substance in the leaves of G. rigescens after spraying the optimal concentration of methyl jasmonate for different time. The levels of relevant biochemical indexes (SOD， POD， CAT， MDA) were determined. RESULTS： 10 d after spraying 10， 50， 100， 200 mg/L methyl jasmonate for G. rigescens strains， the contents of 4 kinds of secoiridoid substance in the leaves were increased to different extent. Compared with untreated leaves， 100 mg/L methyl jasmonate had the best effect after spraying， and the contents of gentiopicrin， swertiamain and sweroside in treated leaves were 1.88， 2.36 and 1.87 times of those in untreated leaves， respectively (P<0.05). 2， 4， 6， 8， 10 d after spraying 100 mg/L methyl jasmonate， the contents of 4 kinds of secoiridoid substance in treated leaves were higher than untreated leaves at corresponding stage； the content of secoiridoid had significant difference after spraying for 4 d (P<0.05). The contents of active components in general were relatively high after spraying for 6 d， and the contents of gentiopicrin， swertiamain， sweroside and amarogentin in treated leaves were 1.88， 1.88， 1.47， 1.82 times of those in untreated leaves， respectively (P<0.05). The levels of relevant biochemical indexes in treated leaves were increased significantly since 4 d of spraying， compared with untreated leaves (P<0.05). CONCLUSIONS： After spraying 100 mg/L methyl jasmonate for 6 d， the contents increase of 4 kinds of secoiridoid substance in the leaves of G. rigescens are most obvious， which may be associated with improving the levels of related biochemical indexes., 百拇医药(金琳 李海峰 刘卫红 胡侃)
参见：首页 > 中医药 > 中药专业 > 中草药汇编 > 中药大典 > 根类 > 龙胆