筒鞘蛇菰乙酸乙酯部位的化学成分研究(1)
摘 要 目的：研究筒鞘蛇菰乙酸乙酯部位的化学成分，为进一步丰富该属植物的化学成分及筒鞘蛇菰的开发利用提供参考。方法：以75%乙醇提取筒鞘蛇菰全株得到醇提物，依次用石油醚、二氯甲烷、乙酸乙酯对提取物进行萃取得到相应部位萃取物。通过硅胶柱、凝胶柱、半制备柱对乙酸乙酯部位萃取物中化学成分进行分离纯化，然后结合波谱(质谱、氢谱、碳谱)数据和文献报道对分离得到的化学成分进行结构鉴定。结果：从筒鞘蛇菰乙酸乙酯部位共分离得到了13个化合物，分别鉴定为pyracanthoside(1)、5，7，3′ ，5′ -四羟基二氢黄酮(2)、柚皮素(3)、高圣草酚(4)、橙皮素(5)、樱花亭(6)、圣草酚(7)、金鱼草素6-O-β-D-吡喃葡萄糖苷(8)、青霉酸(9)、二氢青霉酸(10)、2-甲基-3-呋喃甲酸(11)、5-hydroxymaltol(12)和5，7-二羟基色原酮(13)，多为二氢黄酮类化合物。化合物2～13为首次从蛇菰属植物中分离得到。结论：本研究丰富了蛇菰属植物的化学成分，并为筒鞘蛇菰的质量评价奠定了一定基础。
关键词 筒鞘蛇菰；化学成分；结构鉴定；二氢黄酮类化合物；乙酸乙酯部位
Study on the Chemical Constituents in the Ethyl Acetate Extract of Balanophora involucrate
WEI Jiangchun，LONG Guoqing，WANG Anhua，JIA Jingming(College of TCM，Shenyang Pharmaceutical University， Shenyang 110016， China)
ABSTRACT OBJECTIVE： To study the chemical constituents in the ethyl acetate extract of Balanophora involucrate， and to provide reference for further enriching chemical constituent of the plant and the development and utilization of B. involucrate. METHODS： The whole plant of B. involucrate was extracted with 75% ethyl alcohol. The extraction was carried out by petroleum ether， dichloromethane， ethyl acetate and n-butyl alcohol in turn. The chemical compounds from ethyl acetate extract part were isolated and purified by silica gel column， gel column and semi-preparative HPLC. The structures were identified on the basis of spectral spectrum (mass spectrum， hydrogen spectrum and carbon spectrum) data and literature reports. RESULTS： Thirteen compounds were isolated from ethyl acetate extract part of B. involucrate， identified respectively as pyracanthoside (1)， 5，7，3′ ，5′ -tetrahydroxyflavanone (2)， naringenin (3)， homoeriodictyol (4)， hesperetin (5)， sakuranetin (6)， eriodyctiol (7)， aureusidin-6-O-β-D-glucopyranoside (8)， penicillic acid (9)， dihydropenicillic acid (10)， 2-methyl-3-foroic acid (11)， 5-hydroxymaltol (12) and 5， 7-dyhydroxy chromone (13). Most of them were dihydroflavones. Compounds 2 to 13 are isolated from Balanophora genus for the first time. CONCLUSIONS： The study enriched the chemical constituents of the Balanophora genus and lays foundation for quality evaluation of B. involucrate.
KEYWORDS Balanophora involucrate； Chemical constituent； Structure identification； Dihydroflavones； Ethyl acetate extract part
筒鞘蛇菰(Balanophora involucrate Hook.f.)為蛇菰科(Banophoraceae)蛇菰属(Balanophora)双子叶多年生寄生肉质草本植物，又名鹿仙草、文王一支笔、鸡心七，分布于全世界热带和亚热带地区，如中国台湾、香港、广东、福建、江西、湖北、四川等地[1]，多生长在海拔1 600～2 000 m的阴湿环境中[2]，常寄生于杜鹃属植物的根上[3]。筒鞘蛇菰全株可入药，但产量较少，多为民间用药。研究表明，筒鞘蛇菰在镇痛抗炎[4]、醒酒保肝[5]、抑菌[6]、抗衰老和抗疲劳[7-8]等方面有显著作用，其中的化学成分主要为黄酮类[9-10]、苯丙素类[11]、三萜类[12]和甾体类[13]。目前，已有文献对筒鞘蛇菰乙酸乙酯部位化学成分进行了报道，但相对集中在极性偏小部分，报道的化合物类型也主要为三萜类和甾体类[1，9，11-12]。本课题组在前期试验中发现，筒鞘蛇菰乙酸乙酯萃取部位具有较好的抗炎活性，为进一步探究其抗炎活性的物质基础，本研究对筒鞘蛇菰乙酸乙酯萃取部位极性偏大的化学成分进行了系统地分离鉴定，以期为明确筒鞘蛇菰抗炎活性成分及探索抗炎药物的先导化合物提供理论依据。, http://www.100md.com(魏江春 龙国清 王安华 贾景明)
关键词 筒鞘蛇菰；化学成分；结构鉴定；二氢黄酮类化合物；乙酸乙酯部位
Study on the Chemical Constituents in the Ethyl Acetate Extract of Balanophora involucrate
WEI Jiangchun，LONG Guoqing，WANG Anhua，JIA Jingming(College of TCM，Shenyang Pharmaceutical University， Shenyang 110016， China)
ABSTRACT OBJECTIVE： To study the chemical constituents in the ethyl acetate extract of Balanophora involucrate， and to provide reference for further enriching chemical constituent of the plant and the development and utilization of B. involucrate. METHODS： The whole plant of B. involucrate was extracted with 75% ethyl alcohol. The extraction was carried out by petroleum ether， dichloromethane， ethyl acetate and n-butyl alcohol in turn. The chemical compounds from ethyl acetate extract part were isolated and purified by silica gel column， gel column and semi-preparative HPLC. The structures were identified on the basis of spectral spectrum (mass spectrum， hydrogen spectrum and carbon spectrum) data and literature reports. RESULTS： Thirteen compounds were isolated from ethyl acetate extract part of B. involucrate， identified respectively as pyracanthoside (1)， 5，7，3′ ，5′ -tetrahydroxyflavanone (2)， naringenin (3)， homoeriodictyol (4)， hesperetin (5)， sakuranetin (6)， eriodyctiol (7)， aureusidin-6-O-β-D-glucopyranoside (8)， penicillic acid (9)， dihydropenicillic acid (10)， 2-methyl-3-foroic acid (11)， 5-hydroxymaltol (12) and 5， 7-dyhydroxy chromone (13). Most of them were dihydroflavones. Compounds 2 to 13 are isolated from Balanophora genus for the first time. CONCLUSIONS： The study enriched the chemical constituents of the Balanophora genus and lays foundation for quality evaluation of B. involucrate.
KEYWORDS Balanophora involucrate； Chemical constituent； Structure identification； Dihydroflavones； Ethyl acetate extract part
筒鞘蛇菰(Balanophora involucrate Hook.f.)為蛇菰科(Banophoraceae)蛇菰属(Balanophora)双子叶多年生寄生肉质草本植物，又名鹿仙草、文王一支笔、鸡心七，分布于全世界热带和亚热带地区，如中国台湾、香港、广东、福建、江西、湖北、四川等地[1]，多生长在海拔1 600～2 000 m的阴湿环境中[2]，常寄生于杜鹃属植物的根上[3]。筒鞘蛇菰全株可入药，但产量较少，多为民间用药。研究表明，筒鞘蛇菰在镇痛抗炎[4]、醒酒保肝[5]、抑菌[6]、抗衰老和抗疲劳[7-8]等方面有显著作用，其中的化学成分主要为黄酮类[9-10]、苯丙素类[11]、三萜类[12]和甾体类[13]。目前，已有文献对筒鞘蛇菰乙酸乙酯部位化学成分进行了报道，但相对集中在极性偏小部分，报道的化合物类型也主要为三萜类和甾体类[1，9，11-12]。本课题组在前期试验中发现，筒鞘蛇菰乙酸乙酯萃取部位具有较好的抗炎活性，为进一步探究其抗炎活性的物质基础，本研究对筒鞘蛇菰乙酸乙酯萃取部位极性偏大的化学成分进行了系统地分离鉴定，以期为明确筒鞘蛇菰抗炎活性成分及探索抗炎药物的先导化合物提供理论依据。, http://www.100md.com(魏江春 龙国清 王安华 贾景明)