蒙药阿给水提物的抗炎作用及其机制研究(1)
中圖分类号 R285.5 文献标志码 A 文章编号 1001-0408(2020)12-1425-05
DOI 10.6039/j.issn.1001-0408.2020.12.04
摘 要 目的：研究蒙药阿给(即冷蒿地上部分)水提物对小鼠的抗炎作用，并初步探讨其作用机制。方法：取小鼠60只，随机分为模型对照组(水)、阿司匹林组(阳性对照，0.4 g/kg)和阿给水提物高、中、低剂量组(60.0、30.0、15.0 g/kg，以生药量计)，每组12只。连续灌胃给药5 d后，采用2，4-二硝基氯苯诱导小鼠耳肿胀;另取小鼠60只，同法分组并给药后，采用角叉菜胶诱导小鼠足肿胀;计算小鼠耳肿胀度、肿胀抑制率以及足肿胀率，评价阿给水提物的抗炎作用。取小鼠角叉菜胶致炎足跖组织，分别采用酶联免疫吸附测定法检测肿瘤坏死因子α(TNF-α)、白细胞介素1β(IL-1β)和前列腺素E2(PGE2)的含量，采用硫代巴比妥酸微量法测定丙二醛(MDA)含量，采用硝酸还原酶法测定一氧化氮(NO)含量。结果：与模型对照组比较，阿司匹林组和阿给水提物高、中、低剂量组小鼠耳肿胀度均显著降低(P<0.05或P<0.01)，肿胀抑制率分别为72.3%、71.1%、52.6%、36.8%;阿司匹林组和阿给水提物高、中剂量组小鼠在致炎后各时间点的足肿胀率以及阿给水提物低剂量组小鼠在致炎后0.5、4.0 h时的足肿胀率均显著降低(P<0.05或P<0.01);阿司匹林组和阿给水提物高、中剂量组小鼠足跖炎性组织中TNF-α、IL-1β、PGE2、NO和MDA含量均显著降低(P<0.05或P<0.01)。结论：蒙药阿给水提物对2，4-二硝基氯苯致耳肿胀、角叉菜胶致足肿胀模型小鼠均具有显著的抗炎作用，其机制可能与抑制TNF-α、IL-1β和PGE2生成，减少炎性组织中NO和MDA的过量释放有关。
关键词 蒙药阿给;冷蒿;水提物;抗炎作用;机制;小鼠
Study on Anti-inflammatory Effects and Its Mechanism of Water Extract from Inner Mongolia Medicine Agi
XUE Yan1，LIU Xinyuan2，GAO Hongbo2，XUE Yongzhi2(1. School of Pharmacy， Inner Mongolia Medical University，Hohhot 010110，China;2. College of Preclinical and Forensic Medicine，Baotou Medical College，Inner Mongolia Baotou 014040，China)
ABSTRACT OBJECTIVE： To study the anti-inflammatory effects of water extract from Inner Mongolia medicine Agi (i. e. the aerial part of Artemisia frigida)， and to preliminarily explore its mechanism. METHODS： Totally 60 mice were randomly divided into model control group (water)， aspirin group (positive control， 0.4 g/kg)， Agi water extract high-dose， medium-dose and low-dose groups (60.0， 30.0， 15.0 g/kg， by crude medicine)， with 12 mice in each group. After intragastric administration for consecutive 5 d， the ear swelling of mice was induced by 2， 4-dinitrochlorobenzene. Another 60 mice were grouped and admini- stered by the same way， and the foot swelling of mice was induced by carrageenan. The ear swelling degree and inhibitory rate， paw swelling rate were calculated to evaluate the anti-inflammatory effect of Agi water extract. The contents of TNF-α， IL-1β and PGE2 in plantar inflammatory tissue induced by carrageenan in mice were determined by ELISA. The content of MDA was determined by thiobarbituric acid trace method and NO by nitrate reductase method. RESULTS： Compared with model control group， the degrees of ear swelling of mice were decreased significantly in aspirin group and Agi water extract high-dose， medium-dose， low-dose groups (P<0.05 or P<0.01)， and the inhibitory rates of ear swelling were 72.3%， 71.1%， 52.6%， 36.8%， respectively; the paw swelling rates of mice in aspirin group and Agi high-dose，medium-dose groups were decreased significantly， while those were decreased significantly in Agi water extract low-dose group 0.5 h and 4.0 h after inducing inflammation (P<0.05 or P<0.01); the contents of TNF-α， IL-1β， PGE2， NO and MDA in plantar inflammatory tissue of mice in aspirin group and Agi water extract high-dose， medium-dose groups were decreased significantly (P<0.05 or P<0.01). CONCLUSIONS： Inner Mongolia medicine Agi water extract has obvious anti-inflammatory effects on ear swelling induced by 2，4-dinitrochlorobenzene and paw swelling induced by carrageenan， and its mechanism may be related to the inhibition of TNF-α， IL-1β and PGE2 generation， the reduction of NO and MDA excessive release in inflammatory tissues., http://www.100md.com(薛焱 刘欣媛 高洪波 薛永志)
DOI 10.6039/j.issn.1001-0408.2020.12.04
摘 要 目的：研究蒙药阿给(即冷蒿地上部分)水提物对小鼠的抗炎作用，并初步探讨其作用机制。方法：取小鼠60只，随机分为模型对照组(水)、阿司匹林组(阳性对照，0.4 g/kg)和阿给水提物高、中、低剂量组(60.0、30.0、15.0 g/kg，以生药量计)，每组12只。连续灌胃给药5 d后，采用2，4-二硝基氯苯诱导小鼠耳肿胀;另取小鼠60只，同法分组并给药后，采用角叉菜胶诱导小鼠足肿胀;计算小鼠耳肿胀度、肿胀抑制率以及足肿胀率，评价阿给水提物的抗炎作用。取小鼠角叉菜胶致炎足跖组织，分别采用酶联免疫吸附测定法检测肿瘤坏死因子α(TNF-α)、白细胞介素1β(IL-1β)和前列腺素E2(PGE2)的含量，采用硫代巴比妥酸微量法测定丙二醛(MDA)含量，采用硝酸还原酶法测定一氧化氮(NO)含量。结果：与模型对照组比较，阿司匹林组和阿给水提物高、中、低剂量组小鼠耳肿胀度均显著降低(P<0.05或P<0.01)，肿胀抑制率分别为72.3%、71.1%、52.6%、36.8%;阿司匹林组和阿给水提物高、中剂量组小鼠在致炎后各时间点的足肿胀率以及阿给水提物低剂量组小鼠在致炎后0.5、4.0 h时的足肿胀率均显著降低(P<0.05或P<0.01);阿司匹林组和阿给水提物高、中剂量组小鼠足跖炎性组织中TNF-α、IL-1β、PGE2、NO和MDA含量均显著降低(P<0.05或P<0.01)。结论：蒙药阿给水提物对2，4-二硝基氯苯致耳肿胀、角叉菜胶致足肿胀模型小鼠均具有显著的抗炎作用，其机制可能与抑制TNF-α、IL-1β和PGE2生成，减少炎性组织中NO和MDA的过量释放有关。
关键词 蒙药阿给;冷蒿;水提物;抗炎作用;机制;小鼠
Study on Anti-inflammatory Effects and Its Mechanism of Water Extract from Inner Mongolia Medicine Agi
XUE Yan1，LIU Xinyuan2，GAO Hongbo2，XUE Yongzhi2(1. School of Pharmacy， Inner Mongolia Medical University，Hohhot 010110，China;2. College of Preclinical and Forensic Medicine，Baotou Medical College，Inner Mongolia Baotou 014040，China)
ABSTRACT OBJECTIVE： To study the anti-inflammatory effects of water extract from Inner Mongolia medicine Agi (i. e. the aerial part of Artemisia frigida)， and to preliminarily explore its mechanism. METHODS： Totally 60 mice were randomly divided into model control group (water)， aspirin group (positive control， 0.4 g/kg)， Agi water extract high-dose， medium-dose and low-dose groups (60.0， 30.0， 15.0 g/kg， by crude medicine)， with 12 mice in each group. After intragastric administration for consecutive 5 d， the ear swelling of mice was induced by 2， 4-dinitrochlorobenzene. Another 60 mice were grouped and admini- stered by the same way， and the foot swelling of mice was induced by carrageenan. The ear swelling degree and inhibitory rate， paw swelling rate were calculated to evaluate the anti-inflammatory effect of Agi water extract. The contents of TNF-α， IL-1β and PGE2 in plantar inflammatory tissue induced by carrageenan in mice were determined by ELISA. The content of MDA was determined by thiobarbituric acid trace method and NO by nitrate reductase method. RESULTS： Compared with model control group， the degrees of ear swelling of mice were decreased significantly in aspirin group and Agi water extract high-dose， medium-dose， low-dose groups (P<0.05 or P<0.01)， and the inhibitory rates of ear swelling were 72.3%， 71.1%， 52.6%， 36.8%， respectively; the paw swelling rates of mice in aspirin group and Agi high-dose，medium-dose groups were decreased significantly， while those were decreased significantly in Agi water extract low-dose group 0.5 h and 4.0 h after inducing inflammation (P<0.05 or P<0.01); the contents of TNF-α， IL-1β， PGE2， NO and MDA in plantar inflammatory tissue of mice in aspirin group and Agi water extract high-dose， medium-dose groups were decreased significantly (P<0.05 or P<0.01). CONCLUSIONS： Inner Mongolia medicine Agi water extract has obvious anti-inflammatory effects on ear swelling induced by 2，4-dinitrochlorobenzene and paw swelling induced by carrageenan， and its mechanism may be related to the inhibition of TNF-α， IL-1β and PGE2 generation， the reduction of NO and MDA excessive release in inflammatory tissues., http://www.100md.com(薛焱 刘欣媛 高洪波 薛永志)