忍冬种质资源遗传多样性的AFLP分析(3)
[15]周凤琴， 张永清， 张芳， 等. 山东金银花种质资源的调查研究[J]. 山东中医杂志， 2006， 25(4)： 268.
Genetic diversity of germplasm resources of Lonicera japonica by AFLP analysis
GUO Qing-mei1， WANG Ting2， ZHOU Feng-qin1*， LI Jia1， ZHANG Yong-qing1
(1.Shandong University of Traditional Chinese Medicine， Ji′nan 250355， China；2. Jinan Second Municipal Hospital， Ji′nan 250022， China)
[Abstract]Objective： This study aimed to analyze the genetic diversity and genetic relationship of germplasm resources of Lonicera japonica in main producing areas of China and provide reference for developing new varieties of L. japonica. Method： Using 6 primer combinations， 13 germplasm of L. japonica were analyzed by AFLP marker. The genetic distance was worked out by using DPS V3.01 software， and the cluster was conducted based on UPGMA. Result： A total of 435 bands were obtained including 191 polymorphic ones. The average polymorphic frequency was 43.9%. Cluster analysis showed that the relationship of cultivated variety from the same genuine area was near， and the classification result based on AFLP marker of germplasm of L. japonica from Shandong province was basically consistent with those on their morphological character. Conclusion： AFLP marker can indicate the abundant genetic diversity of L. japonica and provide theoretical evidence for reasonable utilization and breeding new cultivar of L. japonica in molecular level.
[Key words]Lonicera japonica； AFLP； germplasm resources； genetic diversity
doi：10.4268/cjcmm20122006
[责任编辑吕冬梅], 百拇医药(郭庆梅， 王婷， 周凤琴， 李佳， 张永清)
Genetic diversity of germplasm resources of Lonicera japonica by AFLP analysis
GUO Qing-mei1， WANG Ting2， ZHOU Feng-qin1*， LI Jia1， ZHANG Yong-qing1
(1.Shandong University of Traditional Chinese Medicine， Ji′nan 250355， China；2. Jinan Second Municipal Hospital， Ji′nan 250022， China)
[Abstract]Objective： This study aimed to analyze the genetic diversity and genetic relationship of germplasm resources of Lonicera japonica in main producing areas of China and provide reference for developing new varieties of L. japonica. Method： Using 6 primer combinations， 13 germplasm of L. japonica were analyzed by AFLP marker. The genetic distance was worked out by using DPS V3.01 software， and the cluster was conducted based on UPGMA. Result： A total of 435 bands were obtained including 191 polymorphic ones. The average polymorphic frequency was 43.9%. Cluster analysis showed that the relationship of cultivated variety from the same genuine area was near， and the classification result based on AFLP marker of germplasm of L. japonica from Shandong province was basically consistent with those on their morphological character. Conclusion： AFLP marker can indicate the abundant genetic diversity of L. japonica and provide theoretical evidence for reasonable utilization and breeding new cultivar of L. japonica in molecular level.
[Key words]Lonicera japonica； AFLP； germplasm resources； genetic diversity
doi：10.4268/cjcmm20122006
[责任编辑吕冬梅], 百拇医药(郭庆梅， 王婷， 周凤琴， 李佳， 张永清)