蟾蜍类药材中吲哚生物碱和蟾毒配基类成分的含量(4)
[Abstract]Objective： To kinds of establish a HPLC method for determining contents of indole alkaloids and bufadienolides contained in toad medicines， and analyze two kinds of components contained in toad venom， toad skin and toad periostracum. Method： As for alkaloids， Nucleosil C18 column was adopted with acetonitrile and water containing 0.5% potassium dihydrogen phosphate (6∶94， adjust pH to 3.2 with phosphate acid) as the mobile phase. The flow rate was 0.8 mL·min-1， the detection wavelength was 275 nm， and the column temperature was 30 ℃. As for bufadienolides， Alltima C18 column was adopted with acetonitrile and water containing 0.3% acetic acid (B) as the mobile phase. The gradient process was as follows： a linear gradient from 28% to 54% acetonitrile in the first 15 min， then kept at 54% for additional 20 min. The flow rate was 0.6 mL·min-1， the detection wavelength was 296 nm， and the column temperature was 30 ℃. Result： The linear ranges were 0.079 6-0.796 μg for serotonin， 0.097 2-1.945 μg for N-methylserotonin， 0.074 4-0.744 μg for N，N-dimethylserotonin， 0.103-2.05 μg for N，N，N-trimethylserotonin， and 0.067 2-0.672 μg for bufothionine， respectively. The average recoveries of serotonin and N-methylserotonin were 98.6% and 91.3%， respectively. The linear ranges of gamabufotalin， bufotalin， bufalin， cinobufagin and resibufogenin were 0.004 83-0.614， 0.007 9-1.006， 0.007 95-1.016， 0.009 7-1.24 and 0.009 6-1.22 μg， respectively， and their average recoveries were 101.6%， 102.5%， 101.0%， 99.1% and 98.9%， respectively. Conclusion： Toad venom has the highest contents of indole alkaloids and bufadienolides， followed by toad skin， and toad periostracum showed the lowest contents and even no detection result.
[Key words] toad venom； toad skin； toad periostracum； indole alkaloid； bufadienolide； HPLC
doi：10.4268/cjcmm20122018
[责任编辑孔晶晶], http://www.100md.com(曲婷,高慧敏,陈两绵,王智民,张启伟,程翼宇)
[Key words] toad venom； toad skin； toad periostracum； indole alkaloid； bufadienolide； HPLC
doi：10.4268/cjcmm20122018
[责任编辑孔晶晶], http://www.100md.com(曲婷,高慧敏,陈两绵,王智民,张启伟,程翼宇)