白花曼陀罗毛状根的诱导及东莨菪碱和莨菪碱的合成(4)
2.Guizhou Police Officer Vocational College， Guiyang 550005， China)
[Abstract] Objective： To establish the hair roots culture system of Datura metel and study the hair roots growth and biosynthesis of scopolamine and hyoscyamine in hair roots culturing system. Method： Direct degermed cotyledon of wild D. metel was infected by Agrobacterium tumefaciens strain C58C1 to obtain hair roots. Growth curves and scopolamine and hyoscyamine biosynthesis curves were determined. The scopolamine and hyoscyamine from different hair roots lines were examined by HPLC. Result： Hair roots induction rate reached 70%. After 25 days cultured in 1/2 MS liquid nutrient medium， the hair roots weight， content of scopolamine and hyoscyamine reached maximum， tow high efficient accumulation hyoscyamine and scopolamine hair roots lines M1 and M2 were obtained. The medial accumulation coefficient of hyoscyamine and scopolamine were 2.53 times and 5.37 times compared with the leaves of wild D. metel respectively. Conclusion： The established hair roots induction and culture system of D. metel provided a foundation for further obtaining scopolamine and hyoscyamine.
[Key words] Datura metel； hair roots； scopolamine； hyoscyamine； HPLC
doi：10.4268/cjcmm20122113
[责任编辑 吕冬梅]
[稿件编号] 20120327016
[基金项目] 贵州省科技基金项目(黔科合J字[2011]2162号)；贵州警官职业学院科技基金项目(10ZDZK03)
[通信作者] *孙敏，教授，博士生导师，Tel：(023)68254061，E-mail：jwcsm@swu.edu.cn
[作者简介] 张显强，博士，Tel：(023)68254061, http://www.100md.com(张显强 罗正伟 张鸿 王凤英 孙际薇 孙敏)
[Abstract] Objective： To establish the hair roots culture system of Datura metel and study the hair roots growth and biosynthesis of scopolamine and hyoscyamine in hair roots culturing system. Method： Direct degermed cotyledon of wild D. metel was infected by Agrobacterium tumefaciens strain C58C1 to obtain hair roots. Growth curves and scopolamine and hyoscyamine biosynthesis curves were determined. The scopolamine and hyoscyamine from different hair roots lines were examined by HPLC. Result： Hair roots induction rate reached 70%. After 25 days cultured in 1/2 MS liquid nutrient medium， the hair roots weight， content of scopolamine and hyoscyamine reached maximum， tow high efficient accumulation hyoscyamine and scopolamine hair roots lines M1 and M2 were obtained. The medial accumulation coefficient of hyoscyamine and scopolamine were 2.53 times and 5.37 times compared with the leaves of wild D. metel respectively. Conclusion： The established hair roots induction and culture system of D. metel provided a foundation for further obtaining scopolamine and hyoscyamine.
[Key words] Datura metel； hair roots； scopolamine； hyoscyamine； HPLC
doi：10.4268/cjcmm20122113
[责任编辑 吕冬梅]
[稿件编号] 20120327016
[基金项目] 贵州省科技基金项目(黔科合J字[2011]2162号)；贵州警官职业学院科技基金项目(10ZDZK03)
[通信作者] *孙敏，教授，博士生导师，Tel：(023)68254061，E-mail：jwcsm@swu.edu.cn
[作者简介] 张显强，博士，Tel：(023)68254061, http://www.100md.com(张显强 罗正伟 张鸿 王凤英 孙际薇 孙敏)