28个不同采集地土茯苓(断面红色、白色)中落新妇苷分析及抗炎作用的比较(3)
HE Xi-cheng， SHUN Qin-wen， DONG Li-sha， GE Xiang-qian， ZHANG Hua， NONG Heng， SHEN Xiao-hua
(Guiyang College of Traditional Chinese Medicine， Guiyang 550002， China)
[Abstract] Objective： Astilbin in 28 Smilax glabra(red and white cross-section) from different sources was determined by HPLC. Pharmacodynamics and component of S. glabra was investigated through inflammation experiment(penetration type). Method： The analysis was performed on a Hypersil ODS2 column(4.6 mm×250 mm，5 μm)with the mobile phase of acetonitrile and 0.1% acetic acid aqueous(21∶79) at a flow rate of 1.0 mL·min-1. The detection wavelength was 291 nm，and the column temperature was 25 ℃. Anti-inflammatory effect was compared from two type cross-section of Smilax glabra in capillary permeability experiment. Result： Linear correlation was good in the range of 0.003 379-4.004 μg，and the average recoveries were 100.1%，101.9%，99.3%，respectively. The content of astilbin in white and red transverse section were 0.19%-2.46% and 2.10%-5.92%，respectively. Anti-inflammatory efficiency of sectioned red and white were were 21% and 32%， respectively. Conclusion： Astilbin content and anti-inflammatory effect is significantly different between red and white transverse section of S. glabra，the content of astilbin is not positively related with anti-inflammatory effect.
[Key word] Smilax glabra； astilbin； RP-HPLC； anti-inflammatory text
doi：10.4268/cjcmm20122319
[责任编辑 孔晶晶], 百拇医药(何席呈 孙庆文 董立莎 葛向前 张华 农亨 沈晓华)
(Guiyang College of Traditional Chinese Medicine， Guiyang 550002， China)
[Abstract] Objective： Astilbin in 28 Smilax glabra(red and white cross-section) from different sources was determined by HPLC. Pharmacodynamics and component of S. glabra was investigated through inflammation experiment(penetration type). Method： The analysis was performed on a Hypersil ODS2 column(4.6 mm×250 mm，5 μm)with the mobile phase of acetonitrile and 0.1% acetic acid aqueous(21∶79) at a flow rate of 1.0 mL·min-1. The detection wavelength was 291 nm，and the column temperature was 25 ℃. Anti-inflammatory effect was compared from two type cross-section of Smilax glabra in capillary permeability experiment. Result： Linear correlation was good in the range of 0.003 379-4.004 μg，and the average recoveries were 100.1%，101.9%，99.3%，respectively. The content of astilbin in white and red transverse section were 0.19%-2.46% and 2.10%-5.92%，respectively. Anti-inflammatory efficiency of sectioned red and white were were 21% and 32%， respectively. Conclusion： Astilbin content and anti-inflammatory effect is significantly different between red and white transverse section of S. glabra，the content of astilbin is not positively related with anti-inflammatory effect.
[Key word] Smilax glabra； astilbin； RP-HPLC； anti-inflammatory text
doi：10.4268/cjcmm20122319
[责任编辑 孔晶晶], 百拇医药(何席呈 孙庆文 董立莎 葛向前 张华 农亨 沈晓华)