山菠菜三萜组分在体外及体内对II相解毒酶的调控作用机制(4)
Regulation mechanism of triterpenoid components from Prunella asiatica on phase Ⅱ detoxifying enzymes in vitro and in vivo
JIN Ping， TAN Xiao-bin， LIU Wen-bo， JIA Xiao-bin
(1. Key Laboratory of New Drug Delivery System of Chinese Materia Medica， Jiangsu Provincial Academy of Traditional Chinese Medicine， Nanjing 210028， China；
2. Department of Pharmaceutics， Jiangsu University， Zhenjiang 212003， China)
[Abstract] To study the effects of triterpenoid components from Prunella asiatica on phase Ⅱ detoxifying enzymes and protein expression in vitro and in vivo. Normal human bronchial epithelial (NHBE) cell model was used in vitro， and the mouse model of Kunming (KM) mice was used in vivo. CDNB assay was used to measure the activity of GST. NADPH and DCIP was used to detect the activity of NQO1. DTNB colorimetric assay was used to detect GSH. Western blot was use to detect the protein expression of NQO1. We found that triterpenoid components from P. asiatica could increase the activity of GST， NQO1 and GSH in NHBE cells and KM mice. NQO1 protein expression can also be increased in vitro. The study suggests that triterpenoid components from P. asiatica can prevent the lung cancer by regulating the body phase Ⅱ detoxification enzyme activity and protein expression.
[Key words] Prunella asiatica； triterpenoid components； GSH； GST； NQO1
doi：10.4268/cjcmm20122327
[责任编辑 张宁宁], 百拇医药(金苹 谭晓斌 刘文博 贾晓斌)
JIN Ping， TAN Xiao-bin， LIU Wen-bo， JIA Xiao-bin
(1. Key Laboratory of New Drug Delivery System of Chinese Materia Medica， Jiangsu Provincial Academy of Traditional Chinese Medicine， Nanjing 210028， China；
2. Department of Pharmaceutics， Jiangsu University， Zhenjiang 212003， China)
[Abstract] To study the effects of triterpenoid components from Prunella asiatica on phase Ⅱ detoxifying enzymes and protein expression in vitro and in vivo. Normal human bronchial epithelial (NHBE) cell model was used in vitro， and the mouse model of Kunming (KM) mice was used in vivo. CDNB assay was used to measure the activity of GST. NADPH and DCIP was used to detect the activity of NQO1. DTNB colorimetric assay was used to detect GSH. Western blot was use to detect the protein expression of NQO1. We found that triterpenoid components from P. asiatica could increase the activity of GST， NQO1 and GSH in NHBE cells and KM mice. NQO1 protein expression can also be increased in vitro. The study suggests that triterpenoid components from P. asiatica can prevent the lung cancer by regulating the body phase Ⅱ detoxification enzyme activity and protein expression.
[Key words] Prunella asiatica； triterpenoid components； GSH； GST； NQO1
doi：10.4268/cjcmm20122327
[责任编辑 张宁宁], 百拇医药(金苹 谭晓斌 刘文博 贾晓斌)