巫山淫羊藿分蘖芽组织培养研究(4)
[Abstract] Objective： To established the rapid tissue propagation system of Epimedium wushanense， in order to provide theoretical basis for industrialized seed cultivation. Method： Tiller buds E. wushanense were used as explants， with MS， B5， WPM as basic media， and added with different concentrations of plant growth regulators such as 6-BA， NAA and GA3， in order to conduct a systematic study on induction and propagation conditions for tiller buds. Result： The suitable method for sterilizing bud was to disinfect with 75% ethanol for 30 s， and then treated with 0.1% HgCl2 for (4+2) min for consecutively twice， which could control the pollution rate below 5% and the survival rate above 75%. The optimal medium for bud induction was WPM+ 6-BA 2.0 mg·L-1 + NAA 0.1 mg·L-1+ GA3 0.5 mg·L-1， with the induction rate of 75.5%； meanwhile， the basic medium and 6-BA showed significant effect on the induction rate. The propagation medium suitable for buds was MS+6-BA 2.0 mg·L-1+ NAA 0.5 mg·L-1， with the propagation rate of 3.3. The optimal growth of rooting medium was 1/2 WPM+IBA 0.5 mg·L-1+ activated carbonwhich(0.05%)， with the rooting rate of 90%， three to six strong seedlings in each plant. Conclusion： The disinfection method suitable for tiller buds and the medium combination suitable for induction， propagation and rooting of adventitious buds are screened out to establish the rapid cultivation system for tiller buds of E. wushanense.
[Key words] Epimedium wushanense； tissue culture； clustered shoots； plant regeneration
doi：10.4268/cjcmm20122409
[责任编辑 孔晶晶], 百拇医药(周海琴,朱国胜,郭巧生,刘作易,周宁)
[Key words] Epimedium wushanense； tissue culture； clustered shoots； plant regeneration
doi：10.4268/cjcmm20122409
[责任编辑 孔晶晶], 百拇医药(周海琴,朱国胜,郭巧生,刘作易,周宁)