白色紫锥菊不定根诱导及咖啡酸衍生物积累研究(4)
Cheong Ju 3617631， Korea)
[Abstract] Objective： To investigate the effect of auxins 2，4-D，IAA，IBA，NAA on induction of adventitious roots as well as that of IBA concentrations on the growth of adventitious roots and the accumulation of caffeic acid derivatives，with test-tube seedling leaves Echinacea pallida as the explant，and cultivate adventitious roots in bioreactors. Result： 1.0 mg·L-1 IBA was found the best for the induction of adventitious roots，with the numer of induced adventitious roots up to 22.5 in each culture dish. Among different concentrations for suspension cultivation of IBA tested，1.0 mg·L-1 IBA was found the most suitable for the growth of adventitious roots and the accumulation of caffeic acid derivatives. In a 5 L balloon type bubble bioreactor，8.98 g·L-1 dry weight was achieved after one month，which was 2.05 times of 4.38 g·L-1 dry weight cultivated in a triangular flask. The content of echinacoside cultivated in a bioreactor was 14.08 mg·g-1 DW，which was 2.4 times of cultivated roots. The contents of chlorogenic acid，chicoric acid and total caffeic acid derivatives were 4.0-25.6 times of ultivated roots. Conclusion： The study can provide high-quality biomedical drugs containing such caffeic acid derivatives as echinacoside for mass production of Echinacea purpurea medicines.
[Key words] Echinacea pallida； adventitious roots； bioreactor； echinacoside； caffeic acid derivative
doi：10.4268/cjcmm20122418
[责任编辑 孔晶晶], 百拇医药(吴春华,黄韬,崔锡花,白基烨)
[Abstract] Objective： To investigate the effect of auxins 2，4-D，IAA，IBA，NAA on induction of adventitious roots as well as that of IBA concentrations on the growth of adventitious roots and the accumulation of caffeic acid derivatives，with test-tube seedling leaves Echinacea pallida as the explant，and cultivate adventitious roots in bioreactors. Result： 1.0 mg·L-1 IBA was found the best for the induction of adventitious roots，with the numer of induced adventitious roots up to 22.5 in each culture dish. Among different concentrations for suspension cultivation of IBA tested，1.0 mg·L-1 IBA was found the most suitable for the growth of adventitious roots and the accumulation of caffeic acid derivatives. In a 5 L balloon type bubble bioreactor，8.98 g·L-1 dry weight was achieved after one month，which was 2.05 times of 4.38 g·L-1 dry weight cultivated in a triangular flask. The content of echinacoside cultivated in a bioreactor was 14.08 mg·g-1 DW，which was 2.4 times of cultivated roots. The contents of chlorogenic acid，chicoric acid and total caffeic acid derivatives were 4.0-25.6 times of ultivated roots. Conclusion： The study can provide high-quality biomedical drugs containing such caffeic acid derivatives as echinacoside for mass production of Echinacea purpurea medicines.
[Key words] Echinacea pallida； adventitious roots； bioreactor； echinacoside； caffeic acid derivative
doi：10.4268/cjcmm20122418
[责任编辑 孔晶晶], 百拇医药(吴春华,黄韬,崔锡花,白基烨)