不同发育阶段对黄芩生长及活性成分积累的影响(4)
compounds in Scutellaria baicalensis
HU Guo-qiang1，2， YUAN Yuan1*， WU Chong1， JIANG Chao1， WANG Zhou-yong1， LIN Shu-fang1， WU Zhi-gang1
(1. Institute of Chinese Materia Medica， China Academy of Chinese Medical Sciences， Beijing 100700， China；
2. School of Chinese Materia Medica， Beijing University of Chinese Medicine， Beijing 100102， China)
[ Abstract ] Objective：To study the developmental phase on the growth and active compounds in Scutellaria baicalensis. Method： Seeds of wild plants were collected from Laiwu and sowed in Fangshan (Beijing) and Laiwu (Shandong). Samples of aerial and underground parts were collected in five growth periods of sprouts， seedlings， flowering， seed drop and withered periods respectively. The length of taproot， fresh weight of root， diameter of taproot and the length of stem were determined. The content of active compounds and total flavonoids were determined by HPLC and ultraviolet spectrophotometry respectively. The transcripted level of PAL1， PAL2， PAL3， C4H， 4CL， CHS， GUS and UBGAT were analyzed with RT-PCR. Result： The results showed that the aerial part of S. baicalensis grew quickly before flowering stage， and the underground part grew mostly between the periods of flowering and withered. In the whole growing developmental periods， the content of total flavonoids was not changed significantly， the content of baicalin was increased gradually and the content of baicalein was decreased gradually. Expression level of PAL and 4CL was the highest in withered period， CHS was increased between flowering and seed drop and decreased in withered period. Conclusion： Seedlings and withered periods may be the key phase affecting the growth and active compounds in S. baicalensis.
[Key words] phenological phase； active compounds； growing development
doi：10.4268/cjcmm20122423
[责任编辑 吕冬梅], http://www.100md.com(胡国强,袁媛,伍翀,蒋超,汪周勇,林淑芳,吴志刚)
HU Guo-qiang1，2， YUAN Yuan1*， WU Chong1， JIANG Chao1， WANG Zhou-yong1， LIN Shu-fang1， WU Zhi-gang1
(1. Institute of Chinese Materia Medica， China Academy of Chinese Medical Sciences， Beijing 100700， China；
2. School of Chinese Materia Medica， Beijing University of Chinese Medicine， Beijing 100102， China)
[ Abstract ] Objective：To study the developmental phase on the growth and active compounds in Scutellaria baicalensis. Method： Seeds of wild plants were collected from Laiwu and sowed in Fangshan (Beijing) and Laiwu (Shandong). Samples of aerial and underground parts were collected in five growth periods of sprouts， seedlings， flowering， seed drop and withered periods respectively. The length of taproot， fresh weight of root， diameter of taproot and the length of stem were determined. The content of active compounds and total flavonoids were determined by HPLC and ultraviolet spectrophotometry respectively. The transcripted level of PAL1， PAL2， PAL3， C4H， 4CL， CHS， GUS and UBGAT were analyzed with RT-PCR. Result： The results showed that the aerial part of S. baicalensis grew quickly before flowering stage， and the underground part grew mostly between the periods of flowering and withered. In the whole growing developmental periods， the content of total flavonoids was not changed significantly， the content of baicalin was increased gradually and the content of baicalein was decreased gradually. Expression level of PAL and 4CL was the highest in withered period， CHS was increased between flowering and seed drop and decreased in withered period. Conclusion： Seedlings and withered periods may be the key phase affecting the growth and active compounds in S. baicalensis.
[Key words] phenological phase； active compounds； growing development
doi：10.4268/cjcmm20122423
[责任编辑 吕冬梅], http://www.100md.com(胡国强,袁媛,伍翀,蒋超,汪周勇,林淑芳,吴志刚)