荧光衍生法测定中药二氧化硫残留量研究(3)
Determination of sulfur dioxide in traditional Chinese
medicine by derivative fluorometry
PENG Yue， LI Xue-lian， YIN Ling， CHEN Hong-ping， FAN Dan-qing， LIU You-ping*
(Laboratory Constructed by PRC and Sichuan Province in Systematically Research and Development
Utilization of Traditional Chinese Medicine Resources， Department of Pharmacy， Chengdu
University of Traditional Chinese Medicine， Chengdu 611137， China)
[Abstract] Objective： To establish a derivative fluorometry method for the determination of sulfur dioxide residues in traditional Chinese medicine. Method： The optimal derivation condition was established. The fluorescence intensity was detected at excitation wavelength of 321 nm， and emission wavelength of 384 nm. Result： A linear relationship was obtained between the fluorescence intensity and the addition of reference substance in the range of 0.999 7-17.99 nmol with a correlation coeffient of 0.999 9， and the average recovery was 102.3% with RSD 4.6%. Conclusion： This method is simple and sensitive with quick and correct result. It can provide a reference for the determination of sulfur dioxide residues in traditional Chinese medicine.
[Key words] fluorescence spectrophotometry； derivative； sulfur dioxide residues； traditional Chinese medicine
doi：10.4268/cjcmm20130214
[责任编辑 孔晶晶], http://www.100md.com(彭月，李雪莲，银玲，陈鸿平，樊丹青，刘友平)
medicine by derivative fluorometry
PENG Yue， LI Xue-lian， YIN Ling， CHEN Hong-ping， FAN Dan-qing， LIU You-ping*
(Laboratory Constructed by PRC and Sichuan Province in Systematically Research and Development
Utilization of Traditional Chinese Medicine Resources， Department of Pharmacy， Chengdu
University of Traditional Chinese Medicine， Chengdu 611137， China)
[Abstract] Objective： To establish a derivative fluorometry method for the determination of sulfur dioxide residues in traditional Chinese medicine. Method： The optimal derivation condition was established. The fluorescence intensity was detected at excitation wavelength of 321 nm， and emission wavelength of 384 nm. Result： A linear relationship was obtained between the fluorescence intensity and the addition of reference substance in the range of 0.999 7-17.99 nmol with a correlation coeffient of 0.999 9， and the average recovery was 102.3% with RSD 4.6%. Conclusion： This method is simple and sensitive with quick and correct result. It can provide a reference for the determination of sulfur dioxide residues in traditional Chinese medicine.
[Key words] fluorescence spectrophotometry； derivative； sulfur dioxide residues； traditional Chinese medicine
doi：10.4268/cjcmm20130214
[责任编辑 孔晶晶], http://www.100md.com(彭月，李雪莲，银玲，陈鸿平，樊丹青，刘友平)