4种中药单体对人胰腺癌PANC—1细胞移植瘤生长的影响及分子机制探讨(3)
Effects of four kinds of Chinese medicine monomer on growth of PANC-1
xenograft tumor and studying of molecular mechanism
PAN Hui-jun 1， NIE Xu-qiang1， LIU Duo 1， BIAN Ka1，2*
(1.Murad Research Institute for Modernized Chinese Medicine， Shanghai University of Traditional
Chinese Medicine， Shanghai 201203， China；
2.Division of Nitric Oxide and Inflammatory Medicine， E-Institute of Shanghai Universities， Shanghai 201203， China)
[Abstract] Objective： The antitumor effects of icarisid Ⅱ， timosaponin A-Ⅲ， neferine and salidroside were studied in PANC-1 xenograft tumor. Method： To establish of the nude mice xenograft tumor model， PANC-1 cells were injected. When the tumor major diameter was reached 3-5 mm， the treatment was initiated. The mice were randomized into vehicle control and treatment groups of six animals per each. Chinese medicine monomer was injected intraperitoneally every day. In 23th day， mice were killed once a day， tumor tissue were isolated and weighed and divided into two parts. One part was fixed with formaldehyde for tissue section and immunohistochemistry， the another of tissue was frozen in liquid nitrogen then in -80 ℃ refrigerator for gene and protein expression analysis. Result： In PANC-1 tumor xenograft experiment， compared with model group， timosaponin A-Ⅲ (1.0 mg·kg-1) exerted significant inhibitory effects on tumor growth. Timosaponin A-Ⅲ suppressed mRNA expressions of VEGF (P<0.05)， reduced protein expressions of VEGF (P<0.05)， activated Caspase-3 protein. Icarisid Ⅱ， neferine and salidroside had not an excelled antitumor effect. Conclusion： Timosaponin A-Ⅲ exerted an excelled antitumor effect. The antitumor mechanisms include anti-angiogenesis， apoptosis promotion.
[Key words] Chinese medicine monomer； vascular endothelial growth factor； apoptosis
doi：10.4268/cjcmm20130220
[责任编辑 张宁宁], 百拇医药(潘会君，聂绪强，刘朵，卞卡)
xenograft tumor and studying of molecular mechanism
PAN Hui-jun 1， NIE Xu-qiang1， LIU Duo 1， BIAN Ka1，2*
(1.Murad Research Institute for Modernized Chinese Medicine， Shanghai University of Traditional
Chinese Medicine， Shanghai 201203， China；
2.Division of Nitric Oxide and Inflammatory Medicine， E-Institute of Shanghai Universities， Shanghai 201203， China)
[Abstract] Objective： The antitumor effects of icarisid Ⅱ， timosaponin A-Ⅲ， neferine and salidroside were studied in PANC-1 xenograft tumor. Method： To establish of the nude mice xenograft tumor model， PANC-1 cells were injected. When the tumor major diameter was reached 3-5 mm， the treatment was initiated. The mice were randomized into vehicle control and treatment groups of six animals per each. Chinese medicine monomer was injected intraperitoneally every day. In 23th day， mice were killed once a day， tumor tissue were isolated and weighed and divided into two parts. One part was fixed with formaldehyde for tissue section and immunohistochemistry， the another of tissue was frozen in liquid nitrogen then in -80 ℃ refrigerator for gene and protein expression analysis. Result： In PANC-1 tumor xenograft experiment， compared with model group， timosaponin A-Ⅲ (1.0 mg·kg-1) exerted significant inhibitory effects on tumor growth. Timosaponin A-Ⅲ suppressed mRNA expressions of VEGF (P<0.05)， reduced protein expressions of VEGF (P<0.05)， activated Caspase-3 protein. Icarisid Ⅱ， neferine and salidroside had not an excelled antitumor effect. Conclusion： Timosaponin A-Ⅲ exerted an excelled antitumor effect. The antitumor mechanisms include anti-angiogenesis， apoptosis promotion.
[Key words] Chinese medicine monomer； vascular endothelial growth factor； apoptosis
doi：10.4268/cjcmm20130220
[责任编辑 张宁宁], 百拇医药(潘会君，聂绪强，刘朵，卞卡)