基于UPLC—TQ—MS考察不同树龄果用银杏叶萜内酯含量变化规律(4)
Determination of terpene lactones in Ginkgo biloba leaves in
different ages by UPLC-TQ-MS
YAO Xin， ZHOU Gui-sheng， TANG Yu-ping*， QIAN Ye-fei， SHANG Er-xin， SU Shu-lan， QIAN Da-wei， DUAN Jin-ao
(Jiangsu Key Laboratory for High Technology Research of Traditional Chinese
Medicine Formulae， Nanjing University of Chinese Medicine， Nanjing 210046， China)
[Abstract] Objective： To establish an ultra-high performance liquid chromatography coupled with triple quadrupole mass (UPLC-TQ-MS) for determination of four terpene lactones. Method： Chromatographic separation was carried out on a ACQUITY UPLC BEH C18 column (2.1 mm× 100 mm， 1.7 μm) with isocratic elution of 70% methanol at a flow rate of 0.4 mL·min-1， the column temperature was set at 30 ℃； Waters XevoTM TQ worked in multiple reaction monitoring mode. Result： All calibration curves were linear (r>0.990 3) over the tested ranges. The average recoveries ranged from 98.83% to 103.9% with RSD value below 3.0%. The contents of total terpene lactones in Ginkgo biloba leaves were significantly different in different ages. The contents in the leaves of young ginkgo tree were higher than that in old tree. Conclusion： The method was simple and fast with high precision， sensitivity and repeatability， which can be used for qualitative and quantitative analysis of terpene lactones in G. biloba leaves.
[Key words] UPLC-TQ-MS； Ginkgo biloba leaves； terpene lactones； tree age
doi：10.4268/cjcmm20130317
[责任编辑 孔晶晶], 百拇医药(姚鑫，周桂生，唐于平，钱叶飞，尚尔鑫，宿树兰，钱大玮，段金廒)
different ages by UPLC-TQ-MS
YAO Xin， ZHOU Gui-sheng， TANG Yu-ping*， QIAN Ye-fei， SHANG Er-xin， SU Shu-lan， QIAN Da-wei， DUAN Jin-ao
(Jiangsu Key Laboratory for High Technology Research of Traditional Chinese
Medicine Formulae， Nanjing University of Chinese Medicine， Nanjing 210046， China)
[Abstract] Objective： To establish an ultra-high performance liquid chromatography coupled with triple quadrupole mass (UPLC-TQ-MS) for determination of four terpene lactones. Method： Chromatographic separation was carried out on a ACQUITY UPLC BEH C18 column (2.1 mm× 100 mm， 1.7 μm) with isocratic elution of 70% methanol at a flow rate of 0.4 mL·min-1， the column temperature was set at 30 ℃； Waters XevoTM TQ worked in multiple reaction monitoring mode. Result： All calibration curves were linear (r>0.990 3) over the tested ranges. The average recoveries ranged from 98.83% to 103.9% with RSD value below 3.0%. The contents of total terpene lactones in Ginkgo biloba leaves were significantly different in different ages. The contents in the leaves of young ginkgo tree were higher than that in old tree. Conclusion： The method was simple and fast with high precision， sensitivity and repeatability， which can be used for qualitative and quantitative analysis of terpene lactones in G. biloba leaves.
[Key words] UPLC-TQ-MS； Ginkgo biloba leaves； terpene lactones； tree age
doi：10.4268/cjcmm20130317
[责任编辑 孔晶晶], 百拇医药(姚鑫，周桂生，唐于平，钱叶飞，尚尔鑫，宿树兰，钱大玮，段金廒)