中药有效成分半抗原抗体应用研究进展(5)
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Advance of studies on application of hapten antibodies
ZHAO Yu-nan
(Basic Medicine School， Nanjing University of Chinese Medicine， Nanjing 210046， China)
[Abstract]Hapten antibodies are active components of traditional Chinese medicines， have been widely applied in all of study fields of traditional Chinese medicine. First， hapten monoclonal antibodies could be designed into ELISA kits for quantitative analysis on the content of effective components in plant crude extracts or biological samples， which be applied for quality control and studies on pharmacokenetics of traditional Chinese medicines. Second， hapten monoclonal antibodies could be coupled with solid-phase carriers to generate immunoaffinity chromatography column， which could be used for knock-out extract preparation or pre-treatment of complicated sampless. Finally， a single-chain variable fragment antibody (scFV) gene segment of effective components of hapten monoclonal antibodies could be transformed into relative plant cells to gain new varieties with high-enrichment effective components， and thus achieve the molecular breeding of medicinal plants.
[Key words]traditional Chinese medicine； effective component； hapten； antibody； artificial antigen
doi：10.4268/cjcmm20130507
[责任编辑陈玲], http://www.100md.com(赵玉男)
Advance of studies on application of hapten antibodies
ZHAO Yu-nan
(Basic Medicine School， Nanjing University of Chinese Medicine， Nanjing 210046， China)
[Abstract]Hapten antibodies are active components of traditional Chinese medicines， have been widely applied in all of study fields of traditional Chinese medicine. First， hapten monoclonal antibodies could be designed into ELISA kits for quantitative analysis on the content of effective components in plant crude extracts or biological samples， which be applied for quality control and studies on pharmacokenetics of traditional Chinese medicines. Second， hapten monoclonal antibodies could be coupled with solid-phase carriers to generate immunoaffinity chromatography column， which could be used for knock-out extract preparation or pre-treatment of complicated sampless. Finally， a single-chain variable fragment antibody (scFV) gene segment of effective components of hapten monoclonal antibodies could be transformed into relative plant cells to gain new varieties with high-enrichment effective components， and thus achieve the molecular breeding of medicinal plants.
[Key words]traditional Chinese medicine； effective component； hapten； antibody； artificial antigen
doi：10.4268/cjcmm20130507
[责任编辑陈玲], http://www.100md.com(赵玉男)