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Constituents of Dendrobium devonianum and their antioxidant activity
ZHANG Ai-lian， YU Min， XU Hong-hua， SI Jin-ping*
(Nurturing Station for the State Key Laboratory of Subtropical Silviculture， Zhejiang Provinical
Strategic Alliance for Technical Innovation in Industry of Dendrobium Officinale
Zhejiang Agricultural and Forestry University， Lin′an 311300， China)
[Abstract] Objective： To study the chemical constituents of Dendrobium devonianum and identify the material basis components of its function， and then provide the basis for development and utilization of D. devonianum. Method： The constituents were separated and purified on the chromatography of silica gel， Sephadex LH-20 and RP-18 silica gel， and then their structures were elucidated based on the spectra data.ABTS method was used to evaluate the free radical scavenging activity of the phenolic compoundsamong them. Result： Nine compounds were isolated and identified as 2，3，4，9-tetrahydro-1H-pyrido[3，4-b]indole-3-carboxylic acid (1)， 2′-deoxythymidine (2)， adenosine (3)， N-trans-p-coumaroyl tyramine(4)， N-trans-p-feruloyl tyramine(5)， 3-methoxy-4-hydroxybenzaldehyde (6)， 4-hydroxybenzaldehyde (7)， 3，4-dihydroxybenzoic acid methyl ester (8)， and 4-hydroxy-3，5-dimethoxybenzoic acid (9). Compound 5 showed good antioxidant activity with IC50 1.61 mmol·L-1， Compound 9 showed weak antioxidant activity with IC50 35.72 mmol·L-1.Conclusion： All these compounds were isolated from D. devonianum for the first time. Among them， compounds 5 and 9 had some antioxidant activity.
[Key words] Dendrobium devonianum； chemical constituents； ABTS； antioxidant activity
doi：10.4268/cjcmm20130614
[责任编辑 吕冬梅], 百拇医药(张爱莲 于敏 徐宏化 斯金平)
Constituents of Dendrobium devonianum and their antioxidant activity
ZHANG Ai-lian， YU Min， XU Hong-hua， SI Jin-ping*
(Nurturing Station for the State Key Laboratory of Subtropical Silviculture， Zhejiang Provinical
Strategic Alliance for Technical Innovation in Industry of Dendrobium Officinale
Zhejiang Agricultural and Forestry University， Lin′an 311300， China)
[Abstract] Objective： To study the chemical constituents of Dendrobium devonianum and identify the material basis components of its function， and then provide the basis for development and utilization of D. devonianum. Method： The constituents were separated and purified on the chromatography of silica gel， Sephadex LH-20 and RP-18 silica gel， and then their structures were elucidated based on the spectra data.ABTS method was used to evaluate the free radical scavenging activity of the phenolic compoundsamong them. Result： Nine compounds were isolated and identified as 2，3，4，9-tetrahydro-1H-pyrido[3，4-b]indole-3-carboxylic acid (1)， 2′-deoxythymidine (2)， adenosine (3)， N-trans-p-coumaroyl tyramine(4)， N-trans-p-feruloyl tyramine(5)， 3-methoxy-4-hydroxybenzaldehyde (6)， 4-hydroxybenzaldehyde (7)， 3，4-dihydroxybenzoic acid methyl ester (8)， and 4-hydroxy-3，5-dimethoxybenzoic acid (9). Compound 5 showed good antioxidant activity with IC50 1.61 mmol·L-1， Compound 9 showed weak antioxidant activity with IC50 35.72 mmol·L-1.Conclusion： All these compounds were isolated from D. devonianum for the first time. Among them， compounds 5 and 9 had some antioxidant activity.
[Key words] Dendrobium devonianum； chemical constituents； ABTS； antioxidant activity
doi：10.4268/cjcmm20130614
[责任编辑 吕冬梅], 百拇医药(张爱莲 于敏 徐宏化 斯金平)