聚合物键合ODS液相色谱—质谱联用法分析大枣中三萜酸含量(3)
[10] Tian S， Shi Y， Yu Q， et al. Determination of oleanolic acid and ursolic acid contents in Ziziphora clinopodioides Lam. by HPLC method[J]. Pharmacogn Mag， 2010， 22(6)：116.
[11] Willy Hesselink， Ruud H N A Schiffer， Peter R Kootstra. Separation of polycyclic aromatic hydrocarbons on a wide-pore polymeric C18 bonded phase[J]. J Chromatogr A， 1995， 697(1/2)：165.
[12] 张采，李佳，张永清. 大枣化学成分研究概况 [J]. 中国现代中药， 2011， 13(11)：49.
[13] 胡芳，赵智慧，刘孟军. HPLC法测定不同枣品种果实中白桦酯酸、齐墩果酸和熊果酸含量[J]. 中国农学通报， 2011， 27(5)：434.
Determination of triterpenoic acids in fruits of Ziziphus jujuba using
HPLC-MS with polymeric ODS column
ZHANG Yong*， ZHOU An， XIE Xiao-mei
(School of Pharmacy， Anhui College of Traditional Chinese Medicine， Hefei 230031， China)
[Abstract] A simple and sensitive method has been developed to simultaneously determine betunilic acid， oleanolic acid and ursolic acid in the fruits of Ziziphus jujuba from different regions by HPLC-MS. This HPLC assay was performed on PAH polymeric C18 bonded stationary phase column with mobile phase contained acetonitrile-water (90∶10) and with negative ESI detection mode. The developed approach was characterized by short time consumption for chromatographic separation， high sensitivity and good reliability so as to meet the requirements for rapid analysis of large-batch fruits of Z. jujuba from different habitats.
[Key words] Jujubae Fructus； polymeric ODS phase； HPLC-MS； triterpenoic acids； betulic acid； oleanolic acid； ursolic acid
doi：10.4268/cjcmm20130615
[责任编辑 孔晶晶], http://www.100md.com(张勇 周安 谢晓梅)
[11] Willy Hesselink， Ruud H N A Schiffer， Peter R Kootstra. Separation of polycyclic aromatic hydrocarbons on a wide-pore polymeric C18 bonded phase[J]. J Chromatogr A， 1995， 697(1/2)：165.
[12] 张采，李佳，张永清. 大枣化学成分研究概况 [J]. 中国现代中药， 2011， 13(11)：49.
[13] 胡芳，赵智慧，刘孟军. HPLC法测定不同枣品种果实中白桦酯酸、齐墩果酸和熊果酸含量[J]. 中国农学通报， 2011， 27(5)：434.
Determination of triterpenoic acids in fruits of Ziziphus jujuba using
HPLC-MS with polymeric ODS column
ZHANG Yong*， ZHOU An， XIE Xiao-mei
(School of Pharmacy， Anhui College of Traditional Chinese Medicine， Hefei 230031， China)
[Abstract] A simple and sensitive method has been developed to simultaneously determine betunilic acid， oleanolic acid and ursolic acid in the fruits of Ziziphus jujuba from different regions by HPLC-MS. This HPLC assay was performed on PAH polymeric C18 bonded stationary phase column with mobile phase contained acetonitrile-water (90∶10) and with negative ESI detection mode. The developed approach was characterized by short time consumption for chromatographic separation， high sensitivity and good reliability so as to meet the requirements for rapid analysis of large-batch fruits of Z. jujuba from different habitats.
[Key words] Jujubae Fructus； polymeric ODS phase； HPLC-MS； triterpenoic acids； betulic acid； oleanolic acid； ursolic acid
doi：10.4268/cjcmm20130615
[责任编辑 孔晶晶], http://www.100md.com(张勇 周安 谢晓梅)