天钩降压胶囊中黄芩苷大鼠在体肠吸收研究(4)
Tiangou Jiangya capsules
LI Ling-jun*， LI Ji， LOU Hua-wei
(School of Pharmacy， Shandong University of Traditional Chinese Medicine， Jinan 250355， China)
[Abstract] Objective： To study in situ intestinal absorption kinetics of baicalin contained in Tiangou Jiangya capsules， and the effect of different intestinal segments， pH value， drug concentration and P-gp inhibitor on the absorption. Method： The in situ intestinal perfusion test was adopted， and HPLC method was used to determine the content of baicalin in samples at different time points. Ultra-violet (UV) spectrophotometry was used to determine the content of phenol red in samples at different time points. Result： When pH value was at 5.0， 6.5， 7.4， the absorption of baicalin was not impacted. P-gp inhibitor verapamil could enhance the absorption of baicalin. When the quality concentration of the test solution ranged between 5-20 g·L-1， the linearity of theabsorption amount of baicalin increased. The absorption kinetic equation of baicalin was Y=-0.073 7X+0.118 7 (r=0.994 8)， Ka 0.073 7 h-1， t1/2 9.40 h. Conclusion： Baicalin is mainly absorbed in colon. The absorption of baicalin shows the first-order kinetics process， with the absorption mechanism of passive diffusion. Baicalin is a substrate for P-gp.
[Key words] Tiangou Jiangya capsules； baicalin； in situ intestinal perfusion； absorption kinetics； absorption mechanism
doi：10.4268/cjcmm20130625
[责任编辑 陈玲], http://www.100md.com(李凌军 李季 娄华伟)
LI Ling-jun*， LI Ji， LOU Hua-wei
(School of Pharmacy， Shandong University of Traditional Chinese Medicine， Jinan 250355， China)
[Abstract] Objective： To study in situ intestinal absorption kinetics of baicalin contained in Tiangou Jiangya capsules， and the effect of different intestinal segments， pH value， drug concentration and P-gp inhibitor on the absorption. Method： The in situ intestinal perfusion test was adopted， and HPLC method was used to determine the content of baicalin in samples at different time points. Ultra-violet (UV) spectrophotometry was used to determine the content of phenol red in samples at different time points. Result： When pH value was at 5.0， 6.5， 7.4， the absorption of baicalin was not impacted. P-gp inhibitor verapamil could enhance the absorption of baicalin. When the quality concentration of the test solution ranged between 5-20 g·L-1， the linearity of theabsorption amount of baicalin increased. The absorption kinetic equation of baicalin was Y=-0.073 7X+0.118 7 (r=0.994 8)， Ka 0.073 7 h-1， t1/2 9.40 h. Conclusion： Baicalin is mainly absorbed in colon. The absorption of baicalin shows the first-order kinetics process， with the absorption mechanism of passive diffusion. Baicalin is a substrate for P-gp.
[Key words] Tiangou Jiangya capsules； baicalin； in situ intestinal perfusion； absorption kinetics； absorption mechanism
doi：10.4268/cjcmm20130625
[责任编辑 陈玲], http://www.100md.com(李凌军 李季 娄华伟)