丹参ERF转录因子SmERF1基因的表达分析和亚细胞定位(4)

http://www.100md.com 2013年4月1日 《中国中药杂志》 2013年第7期

     [Abstract] Objective： A SmERF1 gene was isolated from Salvia miltiorrhiza， and expression patterns to different stress condition were analysed in the root tissues of S. miltiorrhiza. Method： The cDNA of SmERF1 gene from S. miltiorrhiza was isolated by RT，PCR， and the phylogenetic tree using the neighbour，joining tree method in Mega 5 was obtained. To confirm the protein is likely to localize in the nucleus， the SmERF1 coding region was fused to the N，terminus of the GFP gene under the control of the CaMV 35S promoter and transferred into onion epidermal cells using the particle bombardment method. Semi，quantitative RT，PCR analysis revealed different expression pattern of SmERF1 gene in response to exogenous ABA， MeJA and SA. Result： The phylogenetic tree analysis revealed that SmERF1 is most similar to AP2/ERF Ⅶ subgroup members. The transient expression of the SmERF1：：GFP fusion protein indicated that the SmERF1 was exclusively localized to the nucleus. The transcript of SmERF1 highly accumulated when the plants were treated with MeJA， while accumulated slightly in response to exogenous ABA， salicylic acid. Conclusion： These results suggest hormone such as ABA， MeJA and SA signaling pathways can be involved in the activation and inhibition of the SmERF1.

    [Key words] Salvia miltiorrhiza； ERF； expression analysis； subcellular localization

    doi：10.4268/cjcmm20130705(吴文燕 蒋喜红 刘春生 黄璐琦 申业)

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