艾片经静脉和鼻腔给药的小鼠组织分布研究(3)
intranasal administrations
ZHAO Jing，yi， DU Shou，ying*， LU Yang， WU Hui，chao， LI Hui，yun
[Abstract] Objective： To develop a GC，FID method to determine borneol′s concentration in mouse tissues， and to investigate the tissue distribution after intravenous and intranasal administrations of borneol. Method： Mouse brains， hearts， livers， spleens， lungs and kidneys were collected at 1， 3， 5， 10， 20， 30， 60， 90， 120 min after administration of borneol with the dose of 30.0 mg·kg-1. The drug in tissues was extracted with ethyl acetate， and borneol′s concentration detected by GC， with octadecane as the internal standard. Result： The calibration curve showed a good linear relationship. Extraction recoveries， inter，day and intra，day precisions and stability were in conformity with the analytical requirements of biological samples. Borneol was mainly distributed in most tissues， more in heart， brain and kidney， and less in liver， spleen and lung. Conclusion： The established GC，FID method is applicable for content determination of borneol in tissues. After intravenous and intranasal administrations in mice， borneol is mainly distributed in abundant blood，supply tissues. After intranasal administration， brain tissues showed the highest target coefficient and target effectiveness.
[Key words] borneol； intravenous administration； intranasal administration； tissue distribution
doi：10.4268/cjcmm20130729, http://www.100md.com(赵静宜 杜守颖 陆洋 武慧超 李慧云)
ZHAO Jing，yi， DU Shou，ying*， LU Yang， WU Hui，chao， LI Hui，yun
[Abstract] Objective： To develop a GC，FID method to determine borneol′s concentration in mouse tissues， and to investigate the tissue distribution after intravenous and intranasal administrations of borneol. Method： Mouse brains， hearts， livers， spleens， lungs and kidneys were collected at 1， 3， 5， 10， 20， 30， 60， 90， 120 min after administration of borneol with the dose of 30.0 mg·kg-1. The drug in tissues was extracted with ethyl acetate， and borneol′s concentration detected by GC， with octadecane as the internal standard. Result： The calibration curve showed a good linear relationship. Extraction recoveries， inter，day and intra，day precisions and stability were in conformity with the analytical requirements of biological samples. Borneol was mainly distributed in most tissues， more in heart， brain and kidney， and less in liver， spleen and lung. Conclusion： The established GC，FID method is applicable for content determination of borneol in tissues. After intravenous and intranasal administrations in mice， borneol is mainly distributed in abundant blood，supply tissues. After intranasal administration， brain tissues showed the highest target coefficient and target effectiveness.
[Key words] borneol； intravenous administration； intranasal administration； tissue distribution
doi：10.4268/cjcmm20130729, http://www.100md.com(赵静宜 杜守颖 陆洋 武慧超 李慧云)