马钱子碱血药浓度测定方法的优化与应用(3)
[4] 胡巍，陈军，蔡宝昌，等.马钱子碱经皮给药的镇痛作用[J]. 中华中医药学刊，2008， 26(2)： 385.
[5] 高颖，陈军，蔡宝昌，等.LC法测定大鼠血浆中马钱子碱的浓度[J]. 药物分析杂志，2008，25(2)：2036.
[6] 李静雅，苗凤茹，林力. LC，MS /MS测定马钱子总碱囊泡凝胶经皮给药后大鼠的血药浓度[J].中国中药杂志，2012，37(6)：853.
Optimization and application of method to determine
plasma concentration of brucine
WANG Dong，yue1，2，3， CHEN Jun1，2， CAI Bao，chang2，3*
[Abstract] The HPLC method for determining plasma concentration of brucine was optimized during the study on the effect of the extraction reagent， the extraction frequency and the volume of extraction solvent on the extraction recovery of brucine. The optimum sample treatment method was obtained in the study. Specifically， ammonia water was added， 4 mL extraction solvent (N，hexane，methylene chloride，isopropyl alcohol 65：30：5) were adopted to extract brucine for twice. The method to determine plasma concentration of brucine was applied in pharmacokinetic study to compare pharmacokinetic properties of intravenous injection (5 mg·kg-1) and transdermal administration (40 mg·kg-1) of brucine aqueous alkali. The results showed that both pharmacokinetic parameters of brucine after intravenous injection and transdermal administration were in conformity with the two，compartment model. After transdermal administration， the absolute bioavailability was calculated to be 18.72%. The optimized HPLC method can satisfy the demands of the pharmacokinetic study on brucine.
[Key words] brucine； HPLC； pharmacokinetics, http://www.100md.com(王冬月 陈军 蔡宝昌)
[5] 高颖，陈军，蔡宝昌，等.LC法测定大鼠血浆中马钱子碱的浓度[J]. 药物分析杂志，2008，25(2)：2036.
[6] 李静雅，苗凤茹，林力. LC，MS /MS测定马钱子总碱囊泡凝胶经皮给药后大鼠的血药浓度[J].中国中药杂志，2012，37(6)：853.
Optimization and application of method to determine
plasma concentration of brucine
WANG Dong，yue1，2，3， CHEN Jun1，2， CAI Bao，chang2，3*
[Abstract] The HPLC method for determining plasma concentration of brucine was optimized during the study on the effect of the extraction reagent， the extraction frequency and the volume of extraction solvent on the extraction recovery of brucine. The optimum sample treatment method was obtained in the study. Specifically， ammonia water was added， 4 mL extraction solvent (N，hexane，methylene chloride，isopropyl alcohol 65：30：5) were adopted to extract brucine for twice. The method to determine plasma concentration of brucine was applied in pharmacokinetic study to compare pharmacokinetic properties of intravenous injection (5 mg·kg-1) and transdermal administration (40 mg·kg-1) of brucine aqueous alkali. The results showed that both pharmacokinetic parameters of brucine after intravenous injection and transdermal administration were in conformity with the two，compartment model. After transdermal administration， the absolute bioavailability was calculated to be 18.72%. The optimized HPLC method can satisfy the demands of the pharmacokinetic study on brucine.
[Key words] brucine； HPLC； pharmacokinetics, http://www.100md.com(王冬月 陈军 蔡宝昌)