何首乌快繁技术优化及同源四倍体的诱导与鉴定(3)
(1. Anhui University of Traditional Chinese Medicine， Hefei 230031， China；
2. China Pharmaceutical University， Nanjing 210009， China；
3. National Resource Center for Chinese Materia Medica， China Academy of Chinese Medical Sciences， Beijing 100700， China)
Objective： To establish and optimize the rapid propagation system of Polygonum multiflorum- as well as explore method for induction and identification of autotetraploid.
Method： Propagation medium was optimized by orthogonal test. The buds were immersed in colchicine solution with different concentrations for different time to select induction conditions for autotetraploid of P. multiflorum.
Result： The most appropriate propagation medium was MS medium supplemented with 1.0 mg·L-1 6，BA， 0.3 mg·L-1 NAA， and 0.4 mg·L-1 PP333. That the buds were soaked in 0.2% colchicine solution for 30 h， or soaked in 0.3% colchicine solution for 18 h， was optimal condition to induce autopolyploid of P. multiflorum with induction rate as high as 16.7%.
Conclusion： Rapid propagation of P. multiflorum could be achieved by tissue culture. Furthermore， colchicine was an effective inducer of polyploidy， and 25 tetraploid lines were obtained through chromosome identification. The experiment laid a foundation for the wild resource conservation， superior varieties breeding of P. multiflorum.
[Key words]Polygonum multiflorum； rapid propagation； autotetraploid
doi：10.4268/cjcmm20131002, 百拇医药(黄和平 高山林 王键 黄璐琦 黄鹏)
2. China Pharmaceutical University， Nanjing 210009， China；
3. National Resource Center for Chinese Materia Medica， China Academy of Chinese Medical Sciences， Beijing 100700， China)
Objective： To establish and optimize the rapid propagation system of Polygonum multiflorum- as well as explore method for induction and identification of autotetraploid.
Method： Propagation medium was optimized by orthogonal test. The buds were immersed in colchicine solution with different concentrations for different time to select induction conditions for autotetraploid of P. multiflorum.
Result： The most appropriate propagation medium was MS medium supplemented with 1.0 mg·L-1 6，BA， 0.3 mg·L-1 NAA， and 0.4 mg·L-1 PP333. That the buds were soaked in 0.2% colchicine solution for 30 h， or soaked in 0.3% colchicine solution for 18 h， was optimal condition to induce autopolyploid of P. multiflorum with induction rate as high as 16.7%.
Conclusion： Rapid propagation of P. multiflorum could be achieved by tissue culture. Furthermore， colchicine was an effective inducer of polyploidy， and 25 tetraploid lines were obtained through chromosome identification. The experiment laid a foundation for the wild resource conservation， superior varieties breeding of P. multiflorum.
[Key words]Polygonum multiflorum； rapid propagation； autotetraploid
doi：10.4268/cjcmm20131002, 百拇医药(黄和平 高山林 王键 黄璐琦 黄鹏)