一种基于荧光探针和HK，2细胞的中药肾毒性物质筛查方法及其应用(3)
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An approach to screen nephrotoxic components in traditional Chinese
medicine based on fluorescent probe and HK，2 cells and its application
ZHAO Xiao，ping1，2*， GUO Min2， ZHANG Bo，li1
(1. Tianjin University of Traditional Chinese Medicine， Tianjin 300193， China；
2. Zhejiang Chinese Medical University， Hangzhou 310053， China)
[Abstract]Using fluorescein diacetate labeled HK，2 cells， a fast method for screening nephrotoxic components in traditional Chinese medicines (TCMs) was proposed in this study. The methodology validation showed that the linearity， stability and accuracy of the proposed method were suitable for screening nephrotoxic components in vitro . This method was further applied on screen 352 components from 32 Chinese Pharmacopoeia，indexed toxic TCMs. The results indicated that 31 components from 14 toxic TCMs， including Badou， had significant toxicity on HK，2 cells， which suggested these components may cause nephrotoxicity. The components from the other 18 toxic TCMs had no significant toxicity on HK，2 cells.
[Key words]model of nephrotoxic evaluation； HK，2 cells； fluorescent probe； toxic traditional Chinese medicine
doi：10.4268/cjcmm20131025, http://www.100md.com(赵筱萍 呙敏 张伯礼)
An approach to screen nephrotoxic components in traditional Chinese
medicine based on fluorescent probe and HK，2 cells and its application
ZHAO Xiao，ping1，2*， GUO Min2， ZHANG Bo，li1
(1. Tianjin University of Traditional Chinese Medicine， Tianjin 300193， China；
2. Zhejiang Chinese Medical University， Hangzhou 310053， China)
[Abstract]Using fluorescein diacetate labeled HK，2 cells， a fast method for screening nephrotoxic components in traditional Chinese medicines (TCMs) was proposed in this study. The methodology validation showed that the linearity， stability and accuracy of the proposed method were suitable for screening nephrotoxic components in vitro . This method was further applied on screen 352 components from 32 Chinese Pharmacopoeia，indexed toxic TCMs. The results indicated that 31 components from 14 toxic TCMs， including Badou， had significant toxicity on HK，2 cells， which suggested these components may cause nephrotoxicity. The components from the other 18 toxic TCMs had no significant toxicity on HK，2 cells.
[Key words]model of nephrotoxic evaluation； HK，2 cells； fluorescent probe； toxic traditional Chinese medicine
doi：10.4268/cjcmm20131025, http://www.100md.com(赵筱萍 呙敏 张伯礼)