商陆提取物醋制前后毒性作用的比较研究(3)
(1.Pharmacology College of Nanjing University of Chinese Medicine， Nanjing 210029， China；
2.Jiangsu Key Laboratory of Chinese Medicine Processing， Nanjing 210029， China；
3.Engineering Center of State Ministry of Education for Standardization of Chinese Medicine Processing， Nanjing 210029， China)
Objective： To extract and separate toxic components from Phytolaccae Radix， and to comare the changes in toxicity of Phytolaccae Radix before and after being processed with vinegar.
Method： The mucous membrane irritation response， mouse peritoneal inflammation model and in vitro macrophages release NO model were applied to compared the changes in inflammatory toxicity of toxic components from Phytolaccae Radix before and after being processed with vinegar.
Result： Toxic components of Phytolacca Radix had significant inflammatory toxicity， which could cause conjunctival edema in rabbits， and increase of PGE2 and macrophages release NO content in peritoneal exudate in mice. After being processed with vinegar， they showed reduced irritation， which resulted in decrease of PGE2 and macrophages release NO content in peritoneal exudate in mice.
Conclusion： After being processed with vinegar， the toxicity of toxic components from Phytolacca Radix decreased obviously.
[Key words]Phytolacca Radix； toxic component； mucosa irritation； inflammation
doi：10.4268/cjcmm20131032, 百拇医药(宫乐 吴皓 郁红礼 赵腾斐 徐秋香)
2.Jiangsu Key Laboratory of Chinese Medicine Processing， Nanjing 210029， China；
3.Engineering Center of State Ministry of Education for Standardization of Chinese Medicine Processing， Nanjing 210029， China)
Objective： To extract and separate toxic components from Phytolaccae Radix， and to comare the changes in toxicity of Phytolaccae Radix before and after being processed with vinegar.
Method： The mucous membrane irritation response， mouse peritoneal inflammation model and in vitro macrophages release NO model were applied to compared the changes in inflammatory toxicity of toxic components from Phytolaccae Radix before and after being processed with vinegar.
Result： Toxic components of Phytolacca Radix had significant inflammatory toxicity， which could cause conjunctival edema in rabbits， and increase of PGE2 and macrophages release NO content in peritoneal exudate in mice. After being processed with vinegar， they showed reduced irritation， which resulted in decrease of PGE2 and macrophages release NO content in peritoneal exudate in mice.
Conclusion： After being processed with vinegar， the toxicity of toxic components from Phytolacca Radix decreased obviously.
[Key words]Phytolacca Radix； toxic component； mucosa irritation； inflammation
doi：10.4268/cjcmm20131032, 百拇医药(宫乐 吴皓 郁红礼 赵腾斐 徐秋香)