红景天、蛹虫草、大黄配伍治疗代谢综合征的实验研究Ⅳ——改善肝脏脂质堆积作用(5)
Chinese Academy of Medical Sciences，Beijing 100050，China；
2.Tasly Microcirculation Research Center，Peking University Health Science Center，Beijing 100191，China；
3.State Key Laboratory of Bioactive Substance and Function of Natural Medicines，Institute of Materia Medica，Chinese Academy of Medical Sciences，Beijing 100050，China)
[Abstract] To investigate the effects of a compound(FF16)，compatibility of Rhodiola crenulata，Cordyceps militaris，and Rhum palmatum，on non-alcoholic fatty liver disease in IRF mice induced by high fat diet. H&E stain was processed to evaluate the lipid accumulation in liver，and the dynamic microcirculation observation system was applied to determine hepatic microcirculation of IRF mice. Western blot was performed to evaluate IRS-2 ，HSL and ppar-αexpression in liver. The results demonstrate that FF16 significantly decreased the liver lipid index and improved liver steatosis in IRF mice. Furthermore，FF16 could ameliorate hepatic microcirculation disturbance in IRF mice through enhancing RBC velocity and shear rates by 62.5% and 49.7%，increasing sinusoids perfusion by 70.0%，inhibiting adhered leukocytes in IRF mice. The abnormal expressions of IRS-2 and HSL were both reversed by the administration of FF16. In conclusion，FF16 could improve non-alcoholic fatty liver disease in IRF mice by improving insulin sensitivity，regulating lipid metabolism and improving microcirculation disturbance.
[Key words] traditional Chinese medicine compound FF16； insulin resistance； non-alcoholic fatty liver disease； microcirculation disturbance
doi：10.4268/cjcmm20131128
[责任编辑 陈玲], http://www.100md.com(李娟,张晓琳,刘育英,陈玲,田金英,韩晶岩,张培成,叶菲)
2.Tasly Microcirculation Research Center，Peking University Health Science Center，Beijing 100191，China；
3.State Key Laboratory of Bioactive Substance and Function of Natural Medicines，Institute of Materia Medica，Chinese Academy of Medical Sciences，Beijing 100050，China)
[Abstract] To investigate the effects of a compound(FF16)，compatibility of Rhodiola crenulata，Cordyceps militaris，and Rhum palmatum，on non-alcoholic fatty liver disease in IRF mice induced by high fat diet. H&E stain was processed to evaluate the lipid accumulation in liver，and the dynamic microcirculation observation system was applied to determine hepatic microcirculation of IRF mice. Western blot was performed to evaluate IRS-2 ，HSL and ppar-αexpression in liver. The results demonstrate that FF16 significantly decreased the liver lipid index and improved liver steatosis in IRF mice. Furthermore，FF16 could ameliorate hepatic microcirculation disturbance in IRF mice through enhancing RBC velocity and shear rates by 62.5% and 49.7%，increasing sinusoids perfusion by 70.0%，inhibiting adhered leukocytes in IRF mice. The abnormal expressions of IRS-2 and HSL were both reversed by the administration of FF16. In conclusion，FF16 could improve non-alcoholic fatty liver disease in IRF mice by improving insulin sensitivity，regulating lipid metabolism and improving microcirculation disturbance.
[Key words] traditional Chinese medicine compound FF16； insulin resistance； non-alcoholic fatty liver disease； microcirculation disturbance
doi：10.4268/cjcmm20131128
[责任编辑 陈玲], http://www.100md.com(李娟,张晓琳,刘育英,陈玲,田金英,韩晶岩,张培成,叶菲)