15种中药材表面污染真菌的分离与分子鉴定(4)
Separation and molecular identification of fungal contamination on
surface of 15 Chinese herbal medicines
WANG Wen-li1，2， XU Hui1，2*， CHEN Hui-zhi1，2， ZHENG Run-sheng1，2， TAN Jing1，2， ZHAN Ruo-ting1，2， CHEN Wei-wen1，2
(1.Research Center of Chinese Herbal Resource Science and Engineering，Guangzhou University of Chinese Medicine， Guangzhou 510006， China；
2. Key Laboratory of Chinese Medicinal Resource from Lingnan， Guangzhou University of Chinese Medicine，Ministry of Education， Guangzhou 510006， China)
[Abstract] Objective： To evaluate fungal contamination on the surface of Chinese herbal medicines and explore an appropriate method for fast and efficient identification of contaminant fungi. Method： Chinese herbal medicines were first washed and the washing solution was plated onto potato dextrose agar (PDA) to obtain the pure isolates. For molecular identification， two new pairs of specific primers were designed according to ITS region of fungi genome sequences. The strains were identified through polymerase chain reaction (PCR) and sequence analysis. Result： Fifty fungal strains were obtained from the surface of 15 Chinese herbal medicines with the percent of contaminated samples of 93.3%. Twenty-seven strains among them were successfully identified. Conclusion： Fungal contamination on the surface of Chinese herbal medicines is quite common. Although different fungal species were isolated， the genus Aspergillus was the predominant. The primer pairs developed in this study are compatible and can be used to identify fungal species from the surface of Chinese herbal medicines.
[Key words] Chinese herbal medicines； fungal contamination； polymerase chain reaction (PCR)； molecular identification
doi：10.4268/cjcmm20131213, http://www.100md.com(王文丽 徐晖 陈慧芝 郑润生 谭婧 詹若挺 陈蔚文)
surface of 15 Chinese herbal medicines
WANG Wen-li1，2， XU Hui1，2*， CHEN Hui-zhi1，2， ZHENG Run-sheng1，2， TAN Jing1，2， ZHAN Ruo-ting1，2， CHEN Wei-wen1，2
(1.Research Center of Chinese Herbal Resource Science and Engineering，Guangzhou University of Chinese Medicine， Guangzhou 510006， China；
2. Key Laboratory of Chinese Medicinal Resource from Lingnan， Guangzhou University of Chinese Medicine，Ministry of Education， Guangzhou 510006， China)
[Abstract] Objective： To evaluate fungal contamination on the surface of Chinese herbal medicines and explore an appropriate method for fast and efficient identification of contaminant fungi. Method： Chinese herbal medicines were first washed and the washing solution was plated onto potato dextrose agar (PDA) to obtain the pure isolates. For molecular identification， two new pairs of specific primers were designed according to ITS region of fungi genome sequences. The strains were identified through polymerase chain reaction (PCR) and sequence analysis. Result： Fifty fungal strains were obtained from the surface of 15 Chinese herbal medicines with the percent of contaminated samples of 93.3%. Twenty-seven strains among them were successfully identified. Conclusion： Fungal contamination on the surface of Chinese herbal medicines is quite common. Although different fungal species were isolated， the genus Aspergillus was the predominant. The primer pairs developed in this study are compatible and can be used to identify fungal species from the surface of Chinese herbal medicines.
[Key words] Chinese herbal medicines； fungal contamination； polymerase chain reaction (PCR)； molecular identification
doi：10.4268/cjcmm20131213, http://www.100md.com(王文丽 徐晖 陈慧芝 郑润生 谭婧 詹若挺 陈蔚文)