丹参SmNAC1基因的克隆和生物信息学分析(4)
[15] 康桂娟，黎瑜，夏可灿，等. 巴西橡胶树HbNAC1基因启动子的克隆及序列分析[J]. 基因组学与应用生物学， 2012， 31(5)：447.
Cloning and bioinformatics analysis of SmNAC1 from Salvia miltiorrhiza hairy root
WANG Ya-jun1， JIANG Chao1， ZHAO Rong1， ZHAO Le2， SHEN Ye1*， HUANG Lu-qi1*
(1.National Resource Center for Chinese Materia Medica， China Academy of Chinese Medical Sciences， Beijing 100700， China；
2.College of Pharmaey， Henan University of Traditional Chinese medicine， Zhengzhou 450008， China)
[Abstract] In order to study function of NAC transcription in development， hormone regulation and the stress response of Salvia miltiorrhiza， the NAC transcription was cloned and analyzed. By retrieving cDNA database of S. miltiorrhiza hairy root one NAC unigene was found， then a full length of cDNA was cloned by designing specific primers and PCR amplifying. Using ORF finder it was found that the cDNA containing a NAC-AB conserved domain in N-terminal， so the cDNA was a NAC transcription factor， named as SmNAC1(kF006346). Bioinformatics analysis showed that SmNAC1 had an open reading frame (ORF) of 591 bp encoding 196 amino acids. The calculated protein had isoelectric point (pI) of 4.36 with molecular weight about 21.66 kDa. The transcription level of SmNAC1 after dealing with yeast extract (YE) and silver ion (Ag+) in S. miltiorrhiza hairy root was markedly stimulated up regulating. It was 1.4 fold compared with the control after induction 2 h， and maintained 2.0 fold on 4-12 h after induction. SmNAC1 may participate in regulation of stress response of YE+Ag+.
[Key words] Salvia miltiorrhiza； basic transcription factor； stress responsiveness； bioinformatics analysis
doi：10.4268/cjcmm20131305
[责任编辑 吕冬梅], 百拇医药(王亚君，蒋超，赵，赵乐，申业，黄璐琦)
Cloning and bioinformatics analysis of SmNAC1 from Salvia miltiorrhiza hairy root
WANG Ya-jun1， JIANG Chao1， ZHAO Rong1， ZHAO Le2， SHEN Ye1*， HUANG Lu-qi1*
(1.National Resource Center for Chinese Materia Medica， China Academy of Chinese Medical Sciences， Beijing 100700， China；
2.College of Pharmaey， Henan University of Traditional Chinese medicine， Zhengzhou 450008， China)
[Abstract] In order to study function of NAC transcription in development， hormone regulation and the stress response of Salvia miltiorrhiza， the NAC transcription was cloned and analyzed. By retrieving cDNA database of S. miltiorrhiza hairy root one NAC unigene was found， then a full length of cDNA was cloned by designing specific primers and PCR amplifying. Using ORF finder it was found that the cDNA containing a NAC-AB conserved domain in N-terminal， so the cDNA was a NAC transcription factor， named as SmNAC1(kF006346). Bioinformatics analysis showed that SmNAC1 had an open reading frame (ORF) of 591 bp encoding 196 amino acids. The calculated protein had isoelectric point (pI) of 4.36 with molecular weight about 21.66 kDa. The transcription level of SmNAC1 after dealing with yeast extract (YE) and silver ion (Ag+) in S. miltiorrhiza hairy root was markedly stimulated up regulating. It was 1.4 fold compared with the control after induction 2 h， and maintained 2.0 fold on 4-12 h after induction. SmNAC1 may participate in regulation of stress response of YE+Ag+.
[Key words] Salvia miltiorrhiza； basic transcription factor； stress responsiveness； bioinformatics analysis
doi：10.4268/cjcmm20131305
[责任编辑 吕冬梅], 百拇医药(王亚君，蒋超，赵，赵乐，申业，黄璐琦)