HPLC同时测定正天丸中芍药苷、阿魏酸、升麻素苷、5—O—甲基维斯阿米醇苷的含量(3)
Simultaneous determination of paeoniflorin， ferulic acid， prim-O-glucosylcimifugin and 4′-O-beta-glucopyranosyl-5-O-methylvisamminol in Zhengtian pills by HPLC
HUANG Lan*， CHEN Hui-ling， LI Ling-ling
(Xiamen Institute for Drugs Control， Xiamen 361012， China)
[Abstract] To simultaneously determine paeoniflorin， ferulic acid， prim-O-glucosylcimifugin and 4′-O-beta-glucopyranosyl-5-O-methylvisamminol in Zhengtian pills. In the test， Insertil ODS-C18 column (4.6 mm×250 mm， 5 μm) was adopted， with acetonitrile-0.05% phosphoric acid solution as the mobile phase for gradient elution. The flow rate was 1.0 mL·min-1， the column temperature was 30 ℃ and the detection wavelength was 230 nm. According to the results of the test， paeoniflorin， ferulic acid， prim-O-glucosylcimifugin and 4′-O-beta-glucopyranosyl-5-O-methylvisamminol showed good linear relations between peak areas and sample sizes in 11.37-170.5， 2.188-32.82， 2.896-43.44， and 3.000-45.00 mg·L-1 (r=0.999 9， 0.999 9， 1.000 0， 1.000 0)，respectively. The average recoveries (n=6) were 102.3% (RSD 1.2%)，99.71% (RSD 1.9%)， 101.2% (RSD 1.2%)， and 99.40% (RSD 2.4%)， respectively. The above four components were determined in five batches of samples by using the established method， with satisfactory results. The method was so simple， accurate and highly reproducible that it could be used for quality control of the four components in Zhengtian pills.
[Key words] Chinese patent medicine； Zhengtian pills； paeoniflorin； ferulic acid； prim-O-glucosylcimifugin ； 4′-O-beta-glucopyranosyl-5-O-methylvisamminol； HPLC
doi：10.4268/cjcmm20131316
[责任编辑 曹阳阳], http://www.100md.com(黄澜，陈惠玲，李玲玲)
HUANG Lan*， CHEN Hui-ling， LI Ling-ling
(Xiamen Institute for Drugs Control， Xiamen 361012， China)
[Abstract] To simultaneously determine paeoniflorin， ferulic acid， prim-O-glucosylcimifugin and 4′-O-beta-glucopyranosyl-5-O-methylvisamminol in Zhengtian pills. In the test， Insertil ODS-C18 column (4.6 mm×250 mm， 5 μm) was adopted， with acetonitrile-0.05% phosphoric acid solution as the mobile phase for gradient elution. The flow rate was 1.0 mL·min-1， the column temperature was 30 ℃ and the detection wavelength was 230 nm. According to the results of the test， paeoniflorin， ferulic acid， prim-O-glucosylcimifugin and 4′-O-beta-glucopyranosyl-5-O-methylvisamminol showed good linear relations between peak areas and sample sizes in 11.37-170.5， 2.188-32.82， 2.896-43.44， and 3.000-45.00 mg·L-1 (r=0.999 9， 0.999 9， 1.000 0， 1.000 0)，respectively. The average recoveries (n=6) were 102.3% (RSD 1.2%)，99.71% (RSD 1.9%)， 101.2% (RSD 1.2%)， and 99.40% (RSD 2.4%)， respectively. The above four components were determined in five batches of samples by using the established method， with satisfactory results. The method was so simple， accurate and highly reproducible that it could be used for quality control of the four components in Zhengtian pills.
[Key words] Chinese patent medicine； Zhengtian pills； paeoniflorin； ferulic acid； prim-O-glucosylcimifugin ； 4′-O-beta-glucopyranosyl-5-O-methylvisamminol； HPLC
doi：10.4268/cjcmm20131316
[责任编辑 曹阳阳], http://www.100md.com(黄澜，陈惠玲，李玲玲)